This unique reference/text presents the basic theory and practical applications of metabolic engineering (ME). It offers systematic analysis of complex metabolic pathways and ways of employing recombinant DNA techniques to alter cell behavior, metabolic patterns, and product formation. Treating ME as a distinct subfield of genetic engineering, the book demonstrates new means of enabling cells to produce valuable proteins, polypeptides, and primary and secondary metabolites.
Written by more than 35 leading international experts in the field, this book discusses metabolic engineering in plant and mammalian cells, bacteria, and yeasts and assesses metabolic engineering applications in agriculture, pharmaceuticals, and environmental systems. It illuminates the potential of the "cell factory" model for production of chemicals and therapeutics and examines methods for developing new antiviral and antibacterial molecules and effective gene and somatic-cell therapies. Metabolic Engineering also addresses the use of metabolic flux analysis, metabolic control analysis, and online metabolic flux analysis.
Table of Contents
Introduction to metabolic engineering; metabolic flux balance analysis; engineering of the central metabolism of escherichia coli; metabolic engineering of escherichia coli for the production of aromatic compounds; new tools for metabolic engineering os escherichia coli; metabolic engineering strategies for the production of polyhydroxyalkanoates, a family of biodegradable polymers; amino acid production - principles of metabolic engineering; metabolic engineering of antibiotic biosynthesis for process improvement; the generation of organic molecule diversity through metabolic engineering; On-line metabolic flux analysis for fermentation operation; metabolic engineering of clostridium acetobutylicum; metabolic engineering of yeast for substrate utilization and metabolite production; engineering heterologous secretion in yeast; metabolic engineering for the production of plant secondary metabolites; metabolic engineering of mammalian cells molecular bioremediation - metabolic engineering for biodegradation of recalcitrant pollutants.