Methods in Cell Wall Cytochemistry: 1st Edition (Hardback) book cover

Methods in Cell Wall Cytochemistry

1st Edition

By K V Krishnamurthy

CRC Press

336 pages

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Hardback: 9780849307294
pub: 1999-03-29
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Description

Various methodologies designed to study cell walls are compiled in this book.

Methods in Cell Wall Cytochemistry covers the use of modern dyes, fluorescent chemicals, lectins, and antibody technology (immunocytochemisty.)

Cell wall morphology and chemical composition is covered as well as light and fluorescent cytochemistry; transmission electron microscopic cytochemistry; lectin cytochemistry; and, special emphasis on immunocytochemistry.

Addressing an emerging area of research and technology, this book will appeal to plant pathologists, cell biologists, as well as workers interested in stress response and those employing cell walls for biotechnological research.

Table of Contents

Preface

Cell Wall-Morphology & Chemical Composition

Carbohydrates

Cellulose, Callose and other 1, 3-b-glucans, Chitins, Pectins, Hemicelluloses, Alginic Acid, Sulphated Polysaccharides

Glycoproteins

Structural Proteins-Lectins, Arabinogalacton Rich Proteins (AGP's), Hydroxy Proline Rich Proteins, Proline Rich Proteins, Glycine Rich Proteins, Threonine Rich Proteins, Thionins, Pistil Specific Extensin-Like Proteins

Enzymes-Peroxidase, Polyamineoxidase, Phosphatase, Protease, Nitrate Reductase, Malate Dehydrogenase, Proline Hydroxylase, Glycosylhydrolases and Transferases, Non-Specific Esterases, and Ribonucleuses

Lipids and Related Substances-Cutin, Suberin, Waxes, Sporopollenin, Pollenkitt, and Tryphine

Lignin and Other Phenolic Substances-Lignins, Phenolic Acids and Amides

Mineral Substances-Silica, Calcium, and Boron

Light Microscopic Cytochemistry

Introduction

Tissue Preparation-Use of Fresh Living Tissue, Use of Frozen Tissue, Freeze Substitution and Drying, Chemical Fixation, Dehydration and Clearing, Infiltration and Embedding, Sectioning of Materials

Stains and Staining-Introduction, Control Procedures, Incubation of Materials

Mounting of Stained Materials

Procedures in Tissue Preparation for Light Microscopic Cytochemistry-Freezing, Freeze Substitution and Drying Methods, Preparation of Fixatives, Dehydration and Clearing Procedures

Procedures for the Cytochemical Localizations of Different Cell Wall Substances

Carbohydrates-Insoluble Polysaccharides, Cellulose, Chitin, Callose, Sulphated and Carboxylated polysaccharides

Proteins-Non-Specific Cell Wall Proteins, Enzymic Proteins

Lignins

Sporopollenins

Cutins & Suberin

Minerals

Fluorescent Microscopic Cytochemistry

Introduction

Fluorescent Cytochemical Methods

Cell Wall Carbohydrates

Cell Wall Proteins

Lignin

Ferulic Acid

Sporopollenin

Cutin and Suberin

Transmission Electron Microscopic Cytochemistry

Introduction

Specimen Preparation

Electron Microscopic Staining

TEM Cytochemical Methods

Cell Wall Carbohydrates

Cell Wall Proteins

Cutin and Suberin

Lectin Cytochemistry

Introduction

Procedures Involving Lectin Probes

Immunocytochemistry

Introduction

Basics of Immunocytochemistry-Immunization,

Storing and Purifying Antibodies, Labeling of Antibodies, Tissue Preparation, Immunolabeling, Mounting of Sections

Methods in Immunocytochemistry-Methods in Antibody Production and Tissue Preparation, Application of Immunocytochemistry to Study Cell Wall Components

Literature Cited (at the end of each Chapter)

Subject Categories

BISAC Subject Codes/Headings:
MED008000
MEDICAL / Biochemistry
SCI011000
SCIENCE / Life Sciences / Botany
SCI017000
SCIENCE / Life Sciences / Cytology
SCI086000
SCIENCE / Life Sciences / General