Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery: 1st Edition (Hardback) book cover

Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery

1st Edition

By Paul C.H. Li

CRC Press

528 pages | 4 Color Illus. | 325 B/W Illus.

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pub: 2005-11-01
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The microfluidic lab-on-a-chip allows scientists to conduct chemical and biochemical analysis in a miniaturized format so small that properties and effects are successfully enhanced, and processes seamlessly integrated. This microscale advantage translates into greater sensitivity, more accurate results, and better information.

Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery focuses on all aspects of the microfluidic lab-on-a-chip technologies and offers an overview of the available technology, its limitations, and its breakthroughs over the years. It emphasizes analytical applications of microfluidic technology and offers in-depth coverage of micromachining methods, microfluidic operations, chemical separations, sample preparation and injection methods, detection technology, and various chemical and biological analyses. Other topics of interest include the use of polymeric chips, fluid flow valve and control, single-cell analysis, DNA and RNA amplification techniques, DNA hybridization, immunoassays and enzymatic assays.

Originally conceived as a single chapter published in Ewing's Analytical Instrumentation, this book is a gateway to the vast literature and conference proceedings on the topic. Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery expands upon its roots to present a comprehensive treatment of microfluidic lab-on-a-chip methods and applications for novices and advanced researchers alike.


"…truly an awesome book…This well-documented book with a multitude of references: 1/178 references are integrated throughout the book…well-written and compares well to other science books of its kind…a great book to learn about instrument design, with numerous pictures and illustrations making the text much easier to understand. Good pictures are something that is lacking in all too many science books."

-Gary S. Vardon, Salt Lake Tribune, in Journal of Materials

"In fact I can say that it is an excellent book, well thought out and written."

– P. Myers, in Chromatographia, June 2007, Vol. 65

Table of Contents


Micromachining Methods

Micromachining of Silicon

Micromachining of Glass

Micromachining of Fused Quartz (or Fused Silica)

Micromachining of Polymeric Chips

Metal Patterning

World-to-Chip Interface

Microfluidic Flow

Liquid Pumping Methods

Microfluidic Flow Control

Sample Introduction

Electrokinetic Injection

Hydrodynamic Injection

Other Sample Injection Methods

Sample Pre-concentration

Sample Stacking


Porous Membrane

Other Pre-concentration Methods


Gas Chromatography (GC)

Capillary Electrophoresis (CE)

Chromatographic Separations

Coupled Separations

Detection Methods

Optical Detection Methods

Electrochemical (EC) Detection

Mass Spectrometry (MS)

Other Detection Methods

Applications to Cellular/Particle Analysis

Retention of Cells and Particles

Studies of Cells in a Flow

Other Cell Operations

Applications to Nucleic Acids Analysis

Nucleic Acids Extraction and Purification

Nucleic Acids Amplification

DNA Hybridization

Other Nucleic Acid Applications

Applications to Protein Analysis


Protein Separation

Enzymatic Assays


Problem Sets



Microfluidic Flow

Sample Introduction

Sample Preconcentration



Cellular Analysis

Nucleic Acid Analysis

Protein Analysis



About the Series

Chromatographic Science Series

Learn more…

Subject Categories

BISAC Subject Codes/Headings:
MEDICAL / Pharmacy
SCIENCE / Biotechnology
SCIENCE / Chemistry / Analytic