Molecular Analyses  book cover
1st Edition

Molecular Analyses

ISBN 9780367903718
Published July 11, 2022 by CRC Press
375 Pages 20 Color & 68 B/W Illustrations

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Book Description

DNA and RNA extraction methods from a variety of tissues and samples are now routine, including extraction from single cells. Many methods are now automated. Sequencing efficiency has reached the point where it is now possible to obtain gigabases of data, both quickly and inexpensively. Such methods permit the identification of gene versions, including those associated with disease (e.g. small nucleotide polymorphism analyses, or SNPs). The general public as well as clinicians can now access a wide variety of literature on the molecular bases of diseases, allowing them to better assess disease risks and treatments. This volume concentrates on medically-focused methods, and therefore the major audience will be medical professionals, students, and those involved in medically-related research endeavors. There are also papers in this volume dealing specifically with methods developed to analyze large sequence data sets. Many methods reviewed herein are more broadly applicable to other fields in biology, chemistry, bioinformatics, and bioengineering, and are intended for a broad readership.

Key Features

  • Summarizes nucleic acid extractions from a wide variety of tissues and cells
  • Describes processes of nucleic acid preservation
  • Reviews forensic sampling, detection of nucleic acids, and delivery of nucleic acids to multicellular organisms
  • Provides essential guidance for sequencing, sequence analysis, database searches, and phylogenetic analyses
  • Includes additional methods useful for analysis of nucleic acids and proteins

Related Titles

DeSalle, et al. Phylogenomics: A Primer (ISBN 978-0-3670-2849-7).

Jennings, W. B. Phylogenomic Data Acquisition: Principles and Practice (ISBN 978-0-3678-6980-9).

Wang, X. Next-Generation Sequencing Data Analysis (ISBN 978-1-4822-1788-9)

Sung, W.-K. Algorithms for Next-Generation Sequencing (ISBN 978-0-3676-5797-0)

Table of Contents


List of Contributors

1. Nucleic Acid Extraction from Diverse Samples

Scott O. Rogers

2. DNA Extraction from Mummified Tissues

Yayoi Sato

3. Commercial DNA Extraction Kits

Stuart C. Clarke

4. Automated Nucleic Acid Extraction

Juergen Loeffler, Kathrin D. Schmidt, Holger Hebart, Hermann Einsele

5. Forensic DNA Samples: Collection and Handling

Mark Benecke

6. DNA Preservation

C. William Kilpatrick

7. RNA Storage

Martin Hofmann

8. Disposable Electrochemical DNA Biosensors

Kagan Kerman and Mehmet Ozsoz

9. Handheld Nucleic Acid Analyzer

James A. Higgins

10. DNA-Binding Fluorophores

Mikael Kubista, Jonas Karlsson, Martin Bengtsson, Neven Zoric, Gunar Westman

11. Fluorescence Resonance Energy Transfer (FRET)

Dino A. De Angelis

12. LightUp® Probes

Mikael Leijon

13. Invader® Assay

Stephen Day, Andrea Mast

14. Laser-Capture Microdissection

Falko Fend

15. Helper-Dependent Adenoviral Vectors

Philip Ng

16. Retroviral Vectors

Erin L. Weber, Paula M. Cannon

17. Liposomal Nonviral Delivery Vehicles

Nancy Smyth Templeton

18. Polymer-Based Nonviral Nucleic Acid Delivery and Genome Editing

Janin Germer, Ernst Wagner

19. Automated DNA Sequencing

Alexandre Izmailov

20. DNA Sequencing Methods

Scott O. Rogers

21. Differential Sequencing by Mass Spectrometry

Christian Jurinke, Christiane Honisch, Dirk van den Boom

22. Brief Guide to Conducting Biological Database Searches

John Gray

23. Accessing Genomic Databases

Robert J. Trumbly

24. Phylogenetics, Comparative Genomics, and Phylogenomics

Scott O. Rogers

25. DNannotator: Annotation Software Tool Kit for Regional Genomic Sequences

Chunyu Liu

26. ESTAnnotator: A Tool for High-Throughput EST Annotation

Agnes Hotz-Wagenblatt and Thomas Hankeln

27. The Basics of Omics

Scott O. Rogers

28. Ribotyping

Tim J. Inglis

29. Forensic DNA Typing: Y Chromosome

Lluís Quintana-Murci

30. Forensic Identification: An Overview on Molecular Diagnostic Technology

Adrian Linacre, Yvonne E. Cruickshank

31. Denaturing Gradient Gel Electrophoresis (DGGE)

Luciana Cresta de Barros Dolinsky

32. Heteroduplex Analysis (HA)

Philip L. Beales

33. NEBcutter: A Program to Cleave DNA with Restriction Enzymes

Janos Posfai, R. J. Roberts, Tamas Vincze

34. Differential Display (DD) Analysis

Farid E. Ahmed

35. Protein Truncation Test (PTT)

Sadanand Gite, Mark Lim, Kenneth Rothschild


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Scott Orland Rogers is a professor of molecular biology and evolution at Bowling Green State University, Bowling Green, Ohio. He received his PhD in plant molecular biology from the University of Washington, Seattle. He was an assistant professor and associate professor at the State University of New York College of Environmental Science and Forestry before moving to BGSU. He has taught courses in biology, botany, cell physiology, molecular biology, molecular genetics, bioinformatics, and molecular evolution. Research in his lab includes studies of microbes and nucleic acids preserved in ice, life in extreme environments, group I introns, molecular microbial phylogenetics, microbial metagenomics/metatranscriptomics, ancient DNA, and plant development.