Molecular Biotechnology: Principles and Practices, 1st Edition (Hardback) book cover

Molecular Biotechnology

Principles and Practices, 1st Edition

By Channarayappa

CRC Press

1,228 pages

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Hardback: 9781420051575
pub: 2007-05-30
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Description

Providing a strong base in this emerging and highly promising field, Molecular Biotechnology: Principles and Practice strikes a balance between two important aspects of the science - the theory of molecular biology and the experimental approach to the study of biological processes. The main feature of this book is that it covers a wide range of molecular techniques in biotechnology and is designed to be a student- and teacher-friendly textbook. Each technique is described conceptually, followed by a detailed experimental account of the steps involved. The book can also serve as reference to the interested reader who is venturing into the field of biotechnology for the first time.

Table of Contents

INTRODUCTION TO BIOTECHNOLOGY

Biotechnology: Scope and Importance

Biosafety and Good Laboratory Practices

ADVANCED TECHNIQUES IN MOLECULAR BIOLOGY

Techniques of Cell Fractionation and Centrifugation

Chemical Synthesis of Nucleic Acids

DNA Chip Technology and its Potential Applications

Bioinformatics in Biotechnology

WORKING WITH NUCLEIC ACIDS

Isolation of Nucleic Acids

Measuring Nucleic Acid Concentration and Purity

Electrophoretic Techniques

DNA Sequencing

Genetic Maps and Marker Analysis

Polymerase Chain Reaction (PCR)

In Situ Hybridization

RECOMBINANT DNA AND GENETIC ENGINEERING

Fundamentals of Recombinant DNA Technology

Enzymes in Molecular Cloning

Gene Constructs and Cloning Vectors

DNA Libraries

Molecular Biology of Gene Transfer Systems

Selection and Screening of Recombinant Molecules

APPLICATIONS OF BIOTECHNOLOGY

Genetic Engineering of Microorganisms

Genetic Engineering of Animals

Genetic Engineering in Plants

WORKING WITH PROTEINS

Protein Purification Techniques

Protein Detection and Estimation

Protein Fractionation Techniques

Immunochemical Techniques

BACTERIAL AND MAMMALIAN CELL CULTURE

Biology of Bacteria

Cultivation of Mammalian Cells In

IN VITRO PLANT CELL CULTURE AND CROP IMPROVEMENT

Plant Cell Culture Laboratory and Requirements

Plant Culture Media, Preparation, and Culture Initiation

Micropropagation

Cultures of Organized Tissues

Cultures of Unorganized Tissues

Cryopreservation and Distribution of Clonal Material

Measurement of Plant Cell Growth and Cytological Analysis

Protoplast Fusion and Somaclonal Analysis

Application of Plant Cell, Tissue, and Organ Culture

ENVIRONMENTAL BIOTECHNOLOGY

Biotechnology in Pollution Control

Biodiversity and Genetic Conservation

Bioenergy Fuel from Biomass

Regulatory Aspects of Using Genetically-Modified Organisms

Intellectual Property Rights and Socio-Legal Aspects of Biotechnology

Subject Categories

BISAC Subject Codes/Headings:
MED009000
MEDICAL / Biotechnology
SCI017000
SCIENCE / Life Sciences / Cytology
SCI049000
SCIENCE / Life Sciences / Biology / Molecular Biology