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Molecular Biotechnology
Principles and Practices



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ISBN 9781420051575
Published May 30, 2007 by CRC Press
1228 Pages

 
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Book Description

Providing a strong base in this emerging and highly promising field, Molecular Biotechnology: Principles and Practice strikes a balance between two important aspects of the science - the theory of molecular biology and the experimental approach to the study of biological processes. The main feature of this book is that it covers a wide range of molecular techniques in biotechnology and is designed to be a student- and teacher-friendly textbook. Each technique is described conceptually, followed by a detailed experimental account of the steps involved. The book can also serve as reference to the interested reader who is venturing into the field of biotechnology for the first time.

Table of Contents

INTRODUCTION TO BIOTECHNOLOGY
Biotechnology: Scope and Importance
Biosafety and Good Laboratory Practices

ADVANCED TECHNIQUES IN MOLECULAR BIOLOGY
Techniques of Cell Fractionation and Centrifugation
Chemical Synthesis of Nucleic Acids
DNA Chip Technology and its Potential Applications
Bioinformatics in Biotechnology

WORKING WITH NUCLEIC ACIDS
Isolation of Nucleic Acids
Measuring Nucleic Acid Concentration and Purity
Electrophoretic Techniques
DNA Sequencing
Genetic Maps and Marker Analysis
Polymerase Chain Reaction (PCR)
In Situ Hybridization

RECOMBINANT DNA AND GENETIC ENGINEERING
Fundamentals of Recombinant DNA Technology
Enzymes in Molecular Cloning
Gene Constructs and Cloning Vectors
DNA Libraries
Molecular Biology of Gene Transfer Systems
Selection and Screening of Recombinant Molecules

APPLICATIONS OF BIOTECHNOLOGY
Genetic Engineering of Microorganisms
Genetic Engineering of Animals
Genetic Engineering in Plants

WORKING WITH PROTEINS
Protein Purification Techniques
Protein Detection and Estimation
Protein Fractionation Techniques
Immunochemical Techniques

BACTERIAL AND MAMMALIAN CELL CULTURE
Biology of Bacteria
Cultivation of Mammalian Cells In

IN VITRO PLANT CELL CULTURE AND CROP IMPROVEMENT
Plant Cell Culture Laboratory and Requirements
Plant Culture Media, Preparation, and Culture Initiation
Micropropagation
Cultures of Organized Tissues
Cultures of Unorganized Tissues
Cryopreservation and Distribution of Clonal Material
Measurement of Plant Cell Growth and Cytological Analysis
Protoplast Fusion and Somaclonal Analysis
Application of Plant Cell, Tissue, and Organ Culture

ENVIRONMENTAL BIOTECHNOLOGY
Biotechnology in Pollution Control
Biodiversity and Genetic Conservation
Bioenergy Fuel from Biomass
Regulatory Aspects of Using Genetically-Modified Organisms
Intellectual Property Rights and Socio-Legal Aspects of Biotechnology

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Author(s)

Biography

Channarayappa\,