This book provides readers with a broad view of the field of molecular dosimetry by discussing its origins, underlying concepts, the wide range of approaches to making the measurements and applications for the results. Specific topics include the direct assessment of human carcinogens and assessment of low level risks, molecular epidemiology, validation of molecular epidemiologic methods, quantitative analysis of DNA adducts, the application of fluorescence to analysis of genotoxicity, exposure control versus risk assessment, genetic testing in the workplace, and medical screening for carcinogenesis.
The direct determination of human carcinogens and assessment of low-level risks. Molecular epidemiology: overview of biochemical and molecular basis. Interindividual variation in biotransformation of carcinogens: basis and relevance. Validation of molecular epidemiologic methods. . Toxic exposures in a community setting: the epidemiologic approach. Monitoring of in vivo dose by macromolecular adducts: usefulness in risk estimation. Human tissue culture systems for studies of carcinogen metabolism and macromolecular interactions. 32p-postlabeling analysis of mutagen- and carcinogen-dna adducts and age-related dna modifications (i compounds). DNA-adduct analysis by 32p-postlabeling in the study of human exposure to carcinogens. Quantitative analysis of dna adducts: the potential for mass spectrometric techniques. Analytical approaches for the determination of protein-carcinogen adducts using mass spectrometry. Development of immunoassays for the detection of carcinogen-dna adducts. Immunoassays for the clinically used dna-damaging agents 8-methoxypsoralen and cis-diamminedichloroplatinum (ii). Application of fluorescence to analysis of genotoxicity. Detection of alkylated dna adducts in human tissues. Noninvasive methods for measuring exposure to alkylating agents: recent studies on human subjects. Molecular dosimetry of human aflatoxin exposures. Monitoring exposure to tobacco products by measurement of nicotine metabolites and derived carcinogens. Exposure control versus risk assessment: lessons from the study of genotoxic n-substituted arenes. The bioethics of biomonitoring people for exposure to carcinogens. Genetic testing in the workplace: costs and benefits. Medical screening for carcinogenesis: legal and ethical considerations.