Nanoscopy and Multidimensional Optical Fluorescence Microscopy: 1st Edition (Hardback) book cover

Nanoscopy and Multidimensional Optical Fluorescence Microscopy

1st Edition

Edited by Alberto Diaspro

Chapman and Hall/CRC

448 pages | 26 Color Illus. | 180 B/W Illus.

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Hardback: 9781420078862
pub: 2010-04-26
eBook (VitalSource) : 9780429193088
pub: 2010-04-26
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"Alberto Diaspro has been choreographing light’s dance for over 20 years,

and in Nanoscopy and Multidimensional Optical Fluorescence Microscopy, he has assembled a diverse group of experts to explain the methods they use to coax light to reveal biology’s secrets."

From the Foreword by Daniel Evanko, editor, Nature Methods

Nanoscopy and Multidimensional Optical Fluorescence Microscopy demonstrates that the boundaries between sciences do blur at the bottom, especially those that might separate the optical work of physicists and the cellular work of microbiologists. In 18 chapters written by pioneering researchers, this work offers the first comprehensive and current documentation of the cutting-edge research being accomplished in a wide range of photonic devices with revolutionary application.

The highlight of the book is its coverage of optical nanoscopy and super-resolution microscopy. The rapid advances in this area over the past few years offer researchers in both photonics and molecular biologya wealth of accomplishment upon which they can build.

Offering a complete treatment of this emerging field, this volume:

  • Describes how scientists have exploited the properties of light and its fluorophore partners to overcome the resolution limit of conventional light microscopy
  • Delves into recent ways to minimize the photobleaching that has long hampered many methods including those that have the potential to capture previously unobtainable information on the movements of single molecules
  • Discusses the principles, benefits, and implementation of fluorescence correlation spectroscopy and related methods, which simplifies analysis by limiting light to stationary focal points in a sample
  • Considers the most basic as well as emerging methods for improving three-dimensional optical sectioning microscopy
  • Reviews the basics of FRET (fluorescence resonance energy transfer) and considers its new use for investigating protein complexes

The text also introduces those emerging nonfluorescence microscopy methods that can actually exert mechanical forces to trap and move a variety of objects ranging from beads to living cells and cellular organelles. Combining this technique with fluorescence microscopy provides an unparalleled ability to manipulate and visualize biological samples.

In the half-century since Richard Feynman challenged scientists to come up with the tools to investigate and manipulate our world at the nanoscale, we have succeeded in placing tools in the hands of biophysicists that are leading to major breakthroughs in our understanding of life and our ability to diagnose, treat, and prevent many challenges to human health. This book reflects what has been accomplished to date while pointing the way to what still needs to be done.


This book certainly appears at the right time … . The book should be useful for senior research students in optical microscopy and biophotonics.

—Professor Min Gu, Swinburne University of Technology, Melbourne, Australia

A very useful book for the microscopy field. Alberto Diaspro has done a great job as editor, and I’m sure this book will be appreciated by its readers.

—Professor Peter Saggau, Baylor College of Medicine, Texas, USA

… with over sixty contributing authors, this elegant book should impress many optical manufacturers …

Optical World, August 2010

Table of Contents

STED Microscopy with Compact Light Sources; Lars Kastrup, Dominik Wildanger, Brian Rankin, and Stefan W. Hell

Nonlinear Fluorescence Imaging by Saturated Excitation; Nicholas I. Smith, Shogo Kawano, Masahito Yamanaka, and Katsumasa Fujita

Far-Field Fluorescence Microscopy of Cellular Structures at Molecular Optical Resolution; Christoph Cremer, Alexa von Ketteler, Paul Lemmer, Rainer Kaufmann, Yanina Weiland, Patrick Mueller, M. Hausmann, Manuel Gunkel, Thomas Ruckelshausen, David Baddeley, and Roman Amberger

Fluorescence Microscopy with Extended Depth of Field; Kai Wicker and Rainer Heintzmann

Single Particle Tracking; Kevin Braeckmans, Dries Vercauteren, Jo Demeester, and Stefaan C. De Smedt

Fluorescence Correlation Spectroscopy; Xianke Shi and Thorsten Wohland

Two-Photon Excitation Microscopy: A Superb Wizard for Fluorescence Imaging; Francesca Cella and Alberto Diaspro

Photobleaching Minimization in Single- and Multi-Photon Fluorescence Imaging; Partha Pratim Mondal, Paolo Bianchini, Zeno Lavagnino, and Alberto Diaspro

Applications of Second Harmonic Generation Imaging Microscopy; Paolo Bianchini and Alberto Diaspro

Green Fluorescent Proteins as Intracellular pH Indicators; Fabio Beltram,

Ranieri Bizzarri, Michela Serresi, Stefano Luin, and Michela Serresi

Fluorescence Photoactivation Localization Microscopy; Manasa V. Gudheti, Travis J. Gould, and Samuel T. Hess

Molecular Resolution of Cellular Biochemistry and Physiology by FRET/FLIM; Fred S. Wouters and Gertrude Bunt

FRET-Based Determination of Protein Complex Structure at Nanometer Length Scale in Living Cells; Valerica˘ Raicu

Automation in Multidimensional Fluorescence Microscopy: Novel Instrumentation and Applications in Biomedical Research; Mario Faretta

Optical Manipulation, Photonic Devices, and Their Use in Microscopy; G. Cojoc, C. Liberale, R. Tallerico, A. Puija, M. Moretti, F. Mecarini, G. Das, P. Candeloro, F. De Angelis, and E. Di Fabrizio

Optical Tweezers Microscopy: Piconewton Forces in Cell and Molecular Biology; Francesco Difato, Enrico Ferrari, Rajesh Shahapure, Vincent Torre, and Dan Cojoc

In Vivo Spectroscopic Imaging of Biological Membranes and Surface Imaging for High-Throughput Screening; Jo L. Richens, Peter Weightman, Bill L. Barnes, and Paul O’Shea

Near-Field Optical Microscopy: Insight on the Nanometer-Scale Organization of the Cell Membrane; Davide Normanno, Thomas van Zanten, and María F. García-Parajo

Subject Categories

BISAC Subject Codes/Headings:
SCIENCE / Microscopes & Microscopy
SCIENCE / Optics
SCIENCE / Physics