New Methods in Peptide Mapping for the Characterization of Proteins: 1st Edition (Hardback) book cover

New Methods in Peptide Mapping for the Characterization of Proteins

1st Edition

By William S. Hancock

CRC Press

272 pages

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Hardback: 9780849378225
pub: 1995-10-23
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This text is devoted to the characterization of recombinant DNA-derived proteins by peptide mapping. It describes new technological procedures including capillary electrophoresis, analysis of glycopeptides and the use of electrospray and matrix-assisted laser desorption mass spectrometry. The book presents practical procedures for preparing a protein sample, the enzyme digestion, choice of separation method and procedures for the structural analysis of the separated species. Many figures of peptide maps illustrate typical results. Tables of summary information about digestion, separation conditions, and analyses of important protein samples are also presented.

Table of Contents

Peptide Mapping to Characterize Subtle Modifications in the Analysis of Protein Pharmaceuticals, V.T. Ling, M.L. Eng, P.-J. Lee, R.G. Keck, B.A. Keyt, and E.Canova-Davis

The Optimization of Peptide Mapping via Computer Simulation, L.R. Snyder

The Use of Capillary Electrophoresis for Peptide Mapping of Proteins, E. Rickard and J. Towns

Packed Capillary HPLC-ELectrospray Ionization Mass Spectrometry, J. Stults, B.L. Gillece-Castro, W.J. Henzel, J.H. Bourell, K.L. O'Connell, and L.M. Nuwaysir

Capillary Electrophoresis/Mass Spectrometry in Peptide Mapping, J. Wahl, H. Udseth, and R. Smith

Analyzing Reversed Phase Peptide Maps of Recombinant Glycoproteins, A. Guzzetta, W. Hancock

Matrix-Assisted Laser Desorption Mass Spectrometry for Peptide Mapping, P. Lecchi and R. Caprioli

About the Series

Advances in Analytical Biotechnology

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Subject Categories

BISAC Subject Codes/Headings:
MEDICAL / Biochemistry
SCIENCE / Chemistry / Analytic