This text is devoted to the characterization of recombinant DNA-derived proteins by peptide mapping. It describes new technological procedures including capillary electrophoresis, analysis of glycopeptides and the use of electrospray and matrix-assisted laser desorption mass spectrometry. The book presents practical procedures for preparing a protein sample, the enzyme digestion, choice of separation method and procedures for the structural analysis of the separated species. Many figures of peptide maps illustrate typical results. Tables of summary information about digestion, separation conditions, and analyses of important protein samples are also presented.
Peptide Mapping to Characterize Subtle Modifications in the Analysis of Protein Pharmaceuticals, V.T. Ling, M.L. Eng, P.-J. Lee, R.G. Keck, B.A. Keyt, and E.Canova-Davis
The Optimization of Peptide Mapping via Computer Simulation, L.R. Snyder
The Use of Capillary Electrophoresis for Peptide Mapping of Proteins, E. Rickard and J. Towns
Packed Capillary HPLC-ELectrospray Ionization Mass Spectrometry, J. Stults, B.L. Gillece-Castro, W.J. Henzel, J.H. Bourell, K.L. O'Connell, and L.M. Nuwaysir
Capillary Electrophoresis/Mass Spectrometry in Peptide Mapping, J. Wahl, H. Udseth, and R. Smith
Analyzing Reversed Phase Peptide Maps of Recombinant Glycoproteins, A. Guzzetta, W. Hancock
Matrix-Assisted Laser Desorption Mass Spectrometry for Peptide Mapping, P. Lecchi and R. Caprioli