In regenerative medicine, tissue engineers largely rely on destructive and time-consuming techniques that do not allow in situ and spatial monitoring of tissue growth. Furthermore, once the therapy is implanted in the patient, clinicians are often unable to monitor what is happening in the body. To tackle these barriers, optical techniques have been developed to image and characterize many tissue properties, fabricate tissue engineering scaffolds, and characterize the properties of the scaffolds.
Optical Techniques in Regenerative Medicine illustrates how to use optical imaging techniques and instrumentation for the fabrication, assessment, and longitudinal monitoring of regenerative medicine therapies. The book covers optical coherence tomography, acousto-optic imaging, Raman spectroscopy, machine vision, bioluminescence, second harmonic generation microscopy, multi-photon microscopy, coherent anti-Stokes Raman scattering, fluorescence spectroscopy, and light scattering spectroscopy. Each chapter provides an overview of a particular technique, its advantages and limitations in terms of structural and functional information, and examples of applications in regenerative medicine.
The future evolution of regenerative medicine from academic research to viable clinical alternatives to conventional treatments is dependent on the development of non-destructive analytical techniques that can elucidate the stages of tissue development both in vitro and in vivo as well as track the fate of cells following injection. This practical book demonstrates the vital role of optical techniques in the dynamic field of regenerative medicine. It guides regenerative medicine researchers toward finding the most appropriate technique for their applications and helps biophotonics researchers see where their technologies can be applied.
Introduction and Background
The Role of Optical Techniques in Regenerative Medicine Stephen P. Morgan, Brian C. Wilson, Alex Vitkin, and Felicity R.A.J. Rose
Optical Microscopy in Regenerative Medicine
Fluorescence Microscopy Nicola Green and John W. Haycock
Second-Harmonic Generation Stephen J. Matcher
Two-Photon Polymerization for Tissue-Engineered Scaffold Fabrication Andrew A. Gill and Frederik Claeyssens
In Situ Microscopy Andreas Prediger, Patrick Lindner, Arne Bluma, Kenneth F. Reardon, and Thomas Scheper
Optical Spectroscopy in Regenerative Medicine
Light Scattering as Polarization Spectroscopy Irene Georgakoudi and Martin Hunter
Fluorescence Spectroscopy William R. Lloyd, Leng-Chun Chen, and Mary-Ann Mycek
Raman Spectroscopy Flavius C. Pascut, Andy Downes, and Ioan Notingher
Optical Tomography in Regenerative Medicine
Optical Transillumination and Projection Tomography for Multidimensional Mesoscopic Imaging Jim Swoger, James Sharpe, and Mark A. Haidekker
Optical Coherence Tomography: Overview and Applications Youbo Zhao, Benedikt W. Graf, and Stephen A. Boppart
Application of Polarization-Sensitive OCT and Doppler OCT in Tissue Engineering Ying Yang, Ian Wimpenny, and Ruikang K. Wang
Photoacoustic Tomography Wiendelt Steenbergen
Ultrasound-Modulated Optical Tomography Stephen P. Morgan, Nam T. Huynh, Haowen Ruan, and Felicity R.A.J. Rose
Optical Macroscopic Imaging in Regenerative Medicine
Macroscopic Imaging in Regenerative Medicine Sean J. Kirkpatrick, Stephen J. Matcher, and Stephen P. Morgan
Bioluminescence Imaging Juliaan R.M. van Rappard, Preston Lavinghousez, and Joseph C. Wu