PCR Protocols in Molecular Toxicology  book cover
1st Edition

PCR Protocols in Molecular Toxicology

ISBN 9780849333446
Published September 24, 1997 by CRC Press
252 Pages 3 B/W Illustrations

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Book Description

Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail:

  • Quantitative reverse transcriptase PCR and methods to examine gene expression
  • Differential display cloning
  • Cloning and library screening by PCR
  • Genotype and polymorphism analysis of drug and toxicant metabolizing enzymes
  • Basic, non-PCR based molecular biology methods

    PCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential.
  • Table of Contents

    Basics of the Polymerase Chain Reaction, W.B. Mattes
    Analysis of Gene Expression, J.P. Vanden Heuvel
    Differential Display PCR, J.C. Corton
    Cloning by PCR, J.P. Vanden Heuvel
    Genotype Analysis, D.A. Bell and G. Pittman
    General Molecular Biology Techniques, J.P. Vanden Heuvel
    Appendix I: List of Suppliers
    Appendix II: Companies, Online Catalogs, and Internet Resources

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