PCR Protocols in Molecular Toxicology: 1st Edition (Paperback) book cover

PCR Protocols in Molecular Toxicology

1st Edition

By John P. Vanden Heuvel

CRC Press

256 pages | 3 B/W Illus.

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Paperback: 9780849333446
pub: 1997-09-24
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Description

Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail:

  • Quantitative reverse transcriptase PCR and methods to examine gene expression

  • Differential display cloning

  • Cloning and library screening by PCR

  • Genotype and polymorphism analysis of drug and toxicant metabolizing enzymes

  • Basic, non-PCR based molecular biology methods

    PCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential.

  • Table of Contents

    Basics of the Polymerase Chain Reaction, W.B. Mattes

    Analysis of Gene Expression, J.P. Vanden Heuvel

    Differential Display PCR, J.C. Corton

    Cloning by PCR, J.P. Vanden Heuvel

    Genotype Analysis, D.A. Bell and G. Pittman

    General Molecular Biology Techniques, J.P. Vanden Heuvel

    Appendix I: List of Suppliers

    Appendix II: Companies, Online Catalogs, and Internet Resources

    Index

    About the Series

    Methods in Life Sciences - Toxicology Section

    Learn more…

    Subject Categories

    BISAC Subject Codes/Headings:
    MED008000
    MEDICAL / Biochemistry
    MED096000
    MEDICAL / Toxicology
    SCI049000
    SCIENCE / Life Sciences / Biology / Molecular Biology