Plant Development and Biotechnology: 1st Edition (Hardback) book cover

Plant Development and Biotechnology

1st Edition

Edited by Robert N. Trigiano, Dennis J. Gray

CRC Press

376 pages | 96 B/W Illus.

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Hardback: 9780849316142
pub: 2004-07-28
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pub: 2004-07-28
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Biotechnology revolutionized traditional plant breeding programs. This rapid change produced new discussions on techniques and opportunities for commerce, as well as a fear of the unknown. Plant Development and Biotechnology addresses the major issues of the field, with chapters on broad topics written by specialists. The book applies an informal style that addresses the major aspects of development and biotechnology with minimal references, without sacrificing information or accuracy.

Divided into five primary parts, this volume explores how the field emerged from its early theoretical base to the technical discipline of today. It also covers progress being made with genetically engineered plants, providing a snapshot of the field's controversial present. Part III discusses methods for preparing media, creating solutions and dilutions, and accomplishing sterile culture work. It investigates common methods for visualizing and documenting studies, and quantifying responses of tissue culture in research.

Part IV delivers the essential foundation of plant tissue culture, introducing the three types of commonly used culture regeneration systems. Part V integrates propagation techniques with other methodologies for the modification and manipulation of germplasm.

Part VI concludes with special sections. Subjects include in vitro plant pathology, recent research into genetic and phenotypic variation, the mechanics of commercial plant production, and the importance of clean cultures and problems associated with maintaining in vitro cultures. The final chapter analyzes entrepreneurship in the field and outlines the do's and don'ts to consider when launching an enterprise.


"Many readers will be interested in the material on commercial production of micropropagated plantlets…This book is a pleasure to read, and fledgling plant biotechnicians should be familiar with it. Summing up: Recommended."

-CHOICE, March 2005, Vol. 42, No. 7

"This volume is a detailed and informative review, comprising 29 chapters written by leading workers in their field. …The format of the volume is clear and well-designed… .This is a highly readable volume, likely to be a valuable reference source to both students and established workers in the field of contemporary plant development and biotechnology."

-Mycological Research

Table of Contents

Introduction; Dennis J. Gray and Robert N. Trigiano


History of Plant Tissue and Cell Culture; James D. Caponetti, Dennis J. Gray, and Robert N. Trigiano


Getting Started with Tissue Culture: Media Preparation, Sterile Technique, and Laboratory Equipment; Caula A. Beyl

Histological Techniques; Robert N. Trigiano, Kathleen R. Malueg, Kimberly A. Pickens, Zong-Ming Cheng, and Effin T. Graham

Photographic Methods for Plant Cell and Tissue Culture; Dennis J. Gray

Elements of In Vitro Research ; Michael E. Compton

A Brief Introduction to Plant Anatomy; Robert N. Trigiano and Dennis J. Gray

Plant Growth Regulators in Plant Tissue Culture and Development; Victor P. Gab

Software and Databases as Tools for Analyzing Nucleic Acid and Protein Sequences; Zhijian T. Li

Molecular Approaches to the Study of Plant Development; Albrecht G. von Arnim


Shoot Culture Procedures; Michael E. Kane

Propagation from Nonmeristematic Tissues: Organogenesis; Otto J. Schwarz, Anjuna R. Sharma, and Robert M. Beaty

Molecular Aspects of In Vitro Shoot Organogenesis; Shibo Zhang and Peggy G. Lemaux

Propagation from Nonmeristematic Tissues: Nonzygotic Embryogenesis; Dennis J. Gray

Some Developmental and Molecular Aspects of Somatic Embryogenesis (Nonzygotic Embryogenesis); Andreas Mordhorst, Erika Charbit, and Sacco C. de Vries


Use of Protoplasts for Plant Improvement

Richard E. Veilleux, Michael E. Compton, and James A. Saunders

Haploid Cultures; Sandra M. Reed

Embryo Rescue; Sandra M. Reed

Genetic Engineering Technologies; Zhijian T. Li and Dennis J. Gray

Genetically Modified Plant Controversies: Sensational Headlines versus Pragmatic Research; Harry A. Richards, Laura C. Hudson, Matthew D. Halfhill, and Charles N. Stewart, Jr.

Construction and Use of a Simple Gene Gun for Particle Bombardment; Dennis J. Gray, Michael E. Compton, Ernest Hiebert, Chia Min Lin,

and Victor P. Gaba

A Simple Illumination System for Visualizing Green Fluorescent Protein; Dennis J. Gray, Subramanian Jayasankar, and Zhijian T. Li

Germplasm Preservation; Leigh E. Towill

Valuable Secondary Products from In Vitro Culture; Mary Ann Lila

In Vitro Plant Pathology; Subramanian Jayasankar and D. J. Gray


Variation in Tissue Culture; Subramanian Jayasankar

Commercial Laboratory Production; Gayle R. L. Suttle

Indexing for Plant Pathogens; Alan C. Cassells and Barbara M. Doyle

Entrepreneurship for Biotechnology Ventures: From Bench to Bag; David W. Altman

Subject Categories

BISAC Subject Codes/Headings:
SCIENCE / Life Sciences / Botany
SCIENCE / Life Sciences / Biology / Molecular Biology
TECHNOLOGY & ENGINEERING / Agriculture / General