Polyethylene Glycol as an Embedment for Microscopy and Histochemistry is the first book devoted to discussing polyethylene glycol (PEG) as a biological sample embedment for microscopy (EM and LM). Its contributors are international authorities in all areas of investigation using PEG as an embedding medium. The book provides broad coverage of a variety of methods, including low molecular PEG-cryosection, agarose block section-transferring technique, PEG section for scanning electron microscopy, nucleic acid in situ hybridization study, monoclonal antibody screening, PEG-celloidin mixture embedding method, and diethylene glycol distearate used as removable embedment. This practical volume will interest histologists, histochemists, cytochemists, cell biologists, and other researchers using PEG in microscopy.
An Overview of the Polyethylene Glycols (M.R. Leous). Polyethylene Glycol-Celloidin Mixture for Histological and Histochemical Studies (I.A. Efimov). Polyethylene Glycol in Immunocytochemistry and Enzyme Histochemistry (J. Mowery). PEG-Embedding and Subsequent De-Embedding as a New Method for Multi-Purposes in Electron Microscopy (H. Kondo). Using PEG Sections to Screen Monoclonal Antibodies (J.B.L. Bard and A.S.A. Ross). Application of PEG Sections for In Situ Hybridization Studies (A. Alvarez-Buylla and D.F. Clayton). A New Method for Transfer of Polyethylene Glycol Sections onto Silanated Slides for Immunocytochemistry (K.X. Gao). Diethylene Glycol Distearate (DGD): An Alternative to PEG as a Removable Embedment (D.G. Capco).