Practical Fluorescence Spectroscopy: 1st Edition (Hardback) book cover

Practical Fluorescence Spectroscopy

1st Edition

By Zygmunt Gryczynski, Ignacy Gryczynski

CRC Press

768 pages

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Hardback: 9781439821695
pub: 2019-11-30
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Presenting a detailed, hands-on approach to fluorescence spectroscopy, this book describes experiments that cover basic spectroscopy and advanced aspects of fluorescence spectroscopy. It emphasizes practical guidance, providing background on fundamental concepts as well as guidance on how to handle artifacts, avoid common errors, and interpret data. Nearly 150 experiments from biophysics, biochemistry, and the biomedical sciences demonstrate how methods are applied in practical applications. The result is a hands-on guide to the most important aspects of fluorescence spectroscopy, from steady-state fluorescence to advanced time-resolved fluorescence.

  • Provides a complete overview of nearly 150 experiments using fluorescence spectroscopy, from basic to advanced applications
  • Presents laboratory methods using a variety of instrumental setups with detailed discussion of data analysis and interpretations
  • Covers steady-state phenomena, time-resolved phenomena, and advanced methods
  • Spans biophysical, biochemical, and biomedical applications
  • Describes related concepts, theory, and mathematical background as well as commercially available instruments used for measurements

Table of Contents

Steady-State Phenomena

Absorption and Scattering

Oriented Systems and Electronic Transitions

Conditions for Fluorescence Spectra Measurements

Emission and Excitation Spectra of Various Fluorophores

Solvent Effects: Dipole Moments of Fluorophores

Fluorescence Polarization

Biochemical Applications of Fluorescence

Forster Resonance Energy Transfer (FRET)

Time-Resolved Phenomena (Instruments: Fluorometer)

Conditions for Fluorescence Lifetime Measurements

Time-Resolved Protein Fluorescence

Ultra-Short (picosecond) Fluorescence Lifetime Measurements

Anisotropy Decays

Time-Resolved Emission Spectra

Advanced Techniques and Applications

Multi-Photon Excitation (Ultra-Fast Pulsed Lasers)

Stimulated Emission Depletion (Light Quenching)

Total Internal Reflection Fluorescence (TIRF - Evanescent Wave)

Metal Enhanced Fluorescence (MEF)

Surface Plasmon Coupled Emission (SPCE)

Fluorescence Correlation Spectroscopy (FCS)

Single Molecule Detection (SMD)

Fluorescence-Based Sensing

About the Authors

Ignacy Gryczynski is a professor of cell biology and genetics and the director of the Microscopy Core Facility at the University of North Texas Health Center.

Zygmunt (Karol) Gryczynski is a professor of molecular biology and immunology and the director of the Center for Commercialization of Fluorescence Technologies (CCFT) the University of North Texas Health Center.

Subject Categories

BISAC Subject Codes/Headings:
MEDICAL / Biochemistry
SCIENCE / Chemistry / Industrial & Technical
SCIENCE / Physics