320 pages | 74 Color Illus. | 100 B/W Illus.
Earlier books on the handling of plant chromosomes have not included many of the innovations in cytological techniques for many important crops that have become available in recent years, including information on associating genes with chromosomes. The aim of this book is to compile all the plant cytogenetic techniques, previously published in earlier books, into a laboratory manual. The first part of the book describes standard cytological techniques that are routinely used by students. The second part covers methods used for specific crops for which common cytological methods do not work satisfactorily. The third part discusses cytogenetic techniques (cytology and genetics) for physically locating genes on specific chromosomes. This novel book will be highly useful to students, teachers, and researchers as it is a convenient and comprehensive reference for all plant cytogenetic techniques and protocols.
"In my view, an easily understandable experimental protocol at the classroom level presented through this manual is likely to rejuvenate interest in this fascinating subject the world over for graduate students and teachers alike. I believe that this book should be a must in all the labs and classrooms interested in practicing chromosome techniques."
-Umesh C. Lavania, Department of Botany, Lucknow University
Chapter 1 Introduction
Chapter 2 Conventional Methods for Handling Plant Chromosomes
Chapter 3 Smear Technique for Plant Chromosomes
Chapter 4 Fluorescence In Situ Hybridization
Chapter 5 Flow Analysis and Sorting of Plant Chromosomes
Chapter 6 Pollen Staining
Chapter 7 Cell Division
Chapter 8 Mode of Reproduction in Plants
Chapter 9 Karyotype Analysis
Chapter 10 Classical Methods for Associating Genes with
Chapter 11 Structural Chromosome Changes for Locating the Genes
Chapter 12 Wide Hybridization