Preparative Layer Chromatography: 1st Edition (Hardback) book cover

Preparative Layer Chromatography

1st Edition

Edited by Teresa Kowalska, Joseph Sherma

CRC Press

424 pages | 224 B/W Illus.

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pub: 2006-02-03
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Preparative Layer Chromatography explains how this method is used for separating large quantities of mixtures containing a wide variety of important compounds. It offers a broad review of preparative layer chromatography (PLC) applications and adaptable working procedures for microseparations involving organic, inorganic, and organometallic compounds.

The book contains theoretical background, chemical principles, and relevance of preparative layer chromatography (PLC) to a wide range of applications, particularly in the study of pharmaceuticals and biochemistry. Written by many of the best known and most knowledgeable specialists in the field, the chapters describe all the necessary techniques, current procedures, and superior strategies for selecting the most suitable eluents and designing application-specific PLC systems based on the data being sought. They provide comprehensive instructions, surrounding issues, and suggestions for optimizing optional working techniques within the framework of PLC. The book also provides a complete coverage of bulk sorbents and precoated chromatographic plates available on the international market.

A comprehensive, yet accessible source of information, Preparative Layer Chromatography is a relevant and practical text for experienced as well as novice researchers and practitioners involved in analytical, environmental, geochemical, biological, medicinal, and pharmaceutical analysis.


"…A valuable and important supplement to the existing vast chromatographic literature, [this book] demonstrates the potential of planar chromatography for separation and isolation of pure compounds, even from very complex mixtures."

-Journal of Liquid Chromatography, August 2006

"The contributor[s']…experience integrated in the monograph forms a good basis for the readers."

-Instrumentation Science & Technology, August 2006

"The [first] eight chapters provide sound theoretical and methodological foundations of PLC. Numerous applications of PLC are reviewed in the second section, based on extensive literature. …The application reviews provide numerous examples of solving experimental difficulties for the separation of complex mixtures."

-Preparative Biochemistry & Biotechnology, August 2006

"The book is quite well produced and has a large number of illustrations . . . this volume is a worthy addition to the PLC literature, and a good place to start for the novice."

– I.D. Wilson, in Chromatographia, January 2008, Vol. 67, No. 1/2

Table of Contents


Introduction; Teresa Kowalska and Joseph Sherma

Adsorption Planar Chromatography in the Nonlinear Range: Selected Drawbacks and Selected Guidelines; Krzysztof Kaczmarski, Wojciech Prus please connect the names of all the authorsMieczyslaw Sajewicz, and Teresa Kowalska

Sorbents and Precoated Layers in PLC; Heinz E. Hauck and Michael Schulz

Selection and Optimization of the Mobile Phase for PLC; Virginia Coman

Sample Application and Chromatogram Development; Gertrud E. Morlock

On Methodical Possibilities of the Horizontal Chambers in PLC; Tadeusz H. Dzido and Beata Polak

Location of Separated Zones by Use of Visualization Reagents, UV Absorbance on Layers Containing a Fluorescent Indicator, and Densitometry; Bernd Spangenberg

Additional Detection Methods and Removal of Zones from the Layer; Joseph Sherma


Medical Applications of PLC; Jan Bladek ¸ and Anna Szymanczyk

PLC of Hydrophilic Vitamins; Fumio Watanabe and Emi Miyamoto

Preparative Layer Chromatography of Natural Mixtures; Monika Waksmundzka-Hajnos, Teresa Wawrzynowicz, Michal L. Hajnos, and Grzegorz Józwiak

Application of Preparative Layer Chromatography to Lipids; Weerasinghe M. Indrasena

The Use of PLC for Separation of Natural Pigments; George W. Francis

Application of PLC to Inorganics and Organometallics; Ali Mohammad

PLC in a Cleanup and Group Fractionation of Geochemical Samples: A Review of Commonly Applied Techniques; Monika J. Fabianska

The Use of PLC for Isolation and Identification of Unknown Compounds from the Frankincense Resin (Olibanum): Strategies for Finding Marker Substances; Angelika Koch, Rita Richter, and Simla Basar


About the Series

Chromatographic Science Series

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Subject Categories

BISAC Subject Codes/Headings:
MEDICAL / Biotechnology
SCIENCE / Chemistry / Analytic
SCIENCE / Chemistry / Industrial & Technical