1st Edition

Preparative Scale Chromatography




ISBN 9780824780616
Published October 28, 1988 by CRC Press
344 Pages

USD $350.00

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Book Description

Discusses a model for the propagation of a finite concentration zone in a chromatographic column for the case of a single component sample, and explores operating conditions of ion-exchange chromatography for the production of albumin from bovine serum and plasma.

Table of Contents

1. Numerical Simulation of Band Propagation in Nonlinear Chromatography 2. Line Shapes in Gas Chromatography. Ill. SoluteSolute Interactions in Preparative Columns 3. Simple Considerations on Column Design in Preparative-Scale Liquid Chromatography 4. Optimum Conditions in Preparative Liquid Chromatography. 11. Selection of Column Dimensions 5. Optimization of Injection Conditions in Preparative Liquid Chromatography 6. The Influence of Sample Mass and Flow Velocity on Performance in Preparative Column Liquid Chromatography 7. Preparative Liquid Chromatography and the Hlu Curve 8. Investigation of the Influence of Particle Size on the Productivity of Preparative HPLC Columns 9. Experimental Characterization of Strongly Nonlinear Elution Peaks in Liquid Chromatography 10. Cross-Axis Synchronous Flow-Through Coil Planet Centrifuge Free of Rotary Seals for Preparative Countercurrent Chromatography. Part I. Apparatus and Analysis of Acceleration 11. Cross-Axis Synchronous Flow-Through Coil Planet Centrifuge Free of Rotary Seals for Preparative Countercurrent Chromatography. Part 11. Studies on Phase Distribution and Partition Efficiency in Coaxial Coils 12. The Development and Applications of Preparative-Scale Continuous Chromatography 13. A Method Development Study of the Production of Albumin from Animal Blood by Ion-Exchange Chromatography 14. Preparative Isolation of Purine Oligonucleotides from Partial Hydrolyzates of Depyrimidinated DNA 15. Migration of Zones of Test Dyes in Preparative Thin-Layer Chromatography: Stepwise Gradient Elution

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Author(s)

Biography

Eli Grushka (Hebrew University of Jerusalem, Israel)