Principles and Reactions of Protein Extraction, Purification, and Characterization: 1st Edition (Paperback) book cover

Principles and Reactions of Protein Extraction, Purification, and Characterization

1st Edition

By Hafiz Ahmed, Hafiz Ahmed PhD

CRC Press

408 pages | 160 B/W Illus.

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pub: 2004-09-15
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Description

Principles and Reactions of Protein Extraction, Purification, and Characterization provides the mechanisms and experimental procedures for classic to cutting-edge techniques used in protein extraction, purification, and characterization.

The author presents the principles and reactions behind each procedure and uses tables to compare the different methods. The book also discusses the development of antibodies and immunochemical techniques as tools for characterizing proteins and modified proteins such as glycoproteins. Helpful illustrations, diagrams, and tables effectively transform theoretical concepts into practical knowledge. Along with methodical working procedures for most techniques, the book also offers useful advice on which technique to use and when to apply a particular method.

Presenting the advantages and disadvantages of the various protein techniques, Principles and Reactions of Protein Extraction, Purification, and Characterization enables students and researchers to master the mechanisms behind the protocols and choose the best method for their purposes.

Reviews

"Organized in a logical fashion…the most positive features in each chapter are the occasional placement of actual laboratory procedures (called "working procedures") and discussions on the advantages and disadvantages of each technique/method, often presented in tabular format. This comparison of methods and advice on when certain methods should be used is perhaps the most valuable feature of the book."

- Biochemistry and Molecular Biology Education

Table of Contents

EXTRACTION OF PROTEIN

Preparation of Buffers for Protein Extraction

Use of Protease Inhibitors in Protein Extraction

Use of Detergents in Protein Extraction

Chemical Lysis for Protein Extraction

Mechanical Lysis for Protein Extraction

Preparation of Extract from Prokaryotes

Extraction of Recombinant Protein from Bacteria

Preparation of Extracts from Yeast

Preparation of Extracts from Eukaryotes

Preparation of Extracts from Plants

Preparation of Membrane Extracts

ESTIMATION OF PROTEIN

Ultraviolet Absorption Methods

Colorimetric Methods

Fluorescent Methods

ELECTROPHORETIC ANALYSES OF PROTEIN

Driving Force of Electrophoresis

Polyacrylamide Gel Electrophoresis (PAGE)

Isoelectric Focusing (IEF)

Two-Dimensional (2-D) Gel Electrophoresis

Western Blotting

Capillary Electrophoresis

PURIFICATION OF PROTEIN

General Consideration and Purification Strategy

Non-Chromatographic Purification of Proteins

Chromatographic Purification of Proteins

ANTIBODIES: STRUCTURES, INTERACTIONS AND PRODUCTION

Immune System and Antibody Response

Structure of Antibodies

Antigen-Antibody Interactions

Production oof Antibodies

Development of Monoclonal Antibodies

Purification of Antibodies

ANTIBODY LABELING, ANTIBODY DETECTION AND IMMUNOCHEMICAL TECHNIQUES

Methods of Labeling Antibodies

Detection of Antibody Binding

Immunochemical Techniques: a Wide Range of Sensitive Assays for Detecting and Quantitating (Semi) Antigen-Antibody Reactions

Immunoprecipitation

Immunohistochemistry

Flow Cytometry

PURIFICATION OF GLYCOPROTEINS AND ANALYSES OF THEIR OLIGOSACCHARIDES

Diagrams and Stereochemistry of Monosaccharides and Oligosaccharides

Purification of Glycoproteins

Release of Oligosaccharides From Glycoproteins

Preparation of Glycopeptides by Proteolytic Cleavage

Estimation of Carbohydrates

Chromatographic Separation and Detection of Sugars

Electrophoretic Separation

Glycan Detection/Differentiation by Lectins

Analyses of Carbohydrate Linkage

Subject Categories

BISAC Subject Codes/Headings:
MED008000
MEDICAL / Biochemistry
SCI043000
SCIENCE / Research & Methodology
SCI049000
SCIENCE / Life Sciences / Biology / Molecular Biology