Protein Architecture: Interfacing Molecular Assemblies and Immobilization Biotechnology, 1st Edition (Hardback) book cover

Protein Architecture

Interfacing Molecular Assemblies and Immobilization Biotechnology, 1st Edition

By Lvov/Mohwald

CRC Press

416 pages

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Hardback: 9780824782368
pub: 1999-09-29
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Description

Considers the design, structure and biological activity of ordered films comprised of proteins, polymers, amphiphile molecules and colloidal particles, and assesses the ability of protein/polyion multilayers to mimic living organelles. The book discusses how to reach predetermined locations with proteins and orient molecules while preserving their bioactivity.

Reviews

". . .this is an excellent book that brings this discipline into focus and not only offers a review of principles and concepts on proteins and their organization in surfaces and interfaces, it offers a wealth of experiments and applications. The book accomplishes the goal of the editors, to provide researchers, students in academic institutions and those in industrial companies working on biomaterials, thin organized films, biosensors, protein immobilization and biocompatibility with a valuable source of information. Journal of Texture Studies . . .an excellent book that brings this discipline into focus and not only offers a review of principles and concepts on proteins and their organization in surfaces and interfaces, it offers a wealth of experiments and applications. This book accomplishes the goal of the editors, to provide researchers, students in academic institutions and those in industrial companies working on biomaterials, thin organized films, biosensors, protein immobilization and biocompatibility with a valuable source of information."

---Journal of Food Biochemistry

Table of Contents

Part 1 Protein adsorption on a solid surface: protein adsorption on solid/liquid interface. Part 2 Surface covalent immobilization of proteins: immobilization of protein monolayers on planar solid supports. Part 3 Protein monolayer formation at liquid interface and its transfer on solid substrate: quantitative detection of protein binding to the lipid membrane surface by using quartz crystal microbalance; ordering by capillary forces; Langmuir-Blodgett multilayers of proteins. Part 4 Electrostatic layer-by-layer assembly via alternate adsorption of proteins and polyions: electrostatic layer-by-layer assembly of proteins and polyions; sequential catalysis in organized multienzyme films; fabrication of immunoglobulin mono- and multilayers - their applications for immunosensors; immobilized enzyme multilayers -the way for assembly of microreactors and biosensors; ordered multilayer assemblies - albumin/heparin for biocompatible coating and monoclonal antibodies for optical immunosensors. Part 5 Multilayer assembly with biospecific interaction: biomaterials that communicate; antigen-antibody assembling of enzyme monomolecular layers and multilayers on electrodes. Part 6 Electrochemistry in ordered protein layers: electrochemistry and electroenzymology in layered films containing proteins. Part 7 Patterning of protein layers - two dimensional patterning of proteins.

Subject Categories

BISAC Subject Codes/Headings:
MED008000
MEDICAL / Biochemistry
SCI086000
SCIENCE / Life Sciences / General
TEC003000
TECHNOLOGY & ENGINEERING / Agriculture / General