Protocols for Cytogenetic Mapping of Arthropod Genomes  book cover
1st Edition

Protocols for Cytogenetic Mapping of Arthropod Genomes

Edited By

Igor V. Sharakhov

ISBN 9781466598157
Published October 28, 2014 by CRC Press
526 Pages 91 B/W Illustrations

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Book Description

Arthropods are important to worldwide agriculture, food safety, human health, and energy production. Besides their practical significance, various species represent excellent model systems for biological investigations of evolution, development, physiology, reproduction, and social interaction. For these reasons, arthropod genomics is receiving increasing attention from researchers around the globe.

Protocols for Cytogenetic Mapping of Arthropod Genomes is a collection of up-to-date, detailed protocols for physical chromosome mapping and their applications for studying genome organization and evolution in insects and ticks.

This book brings together the expertise of cytogeneticists working on diverse groups of arthropods. Each chapter demonstrates approaches to tissue dissection, chromosome preparation, fluorescence in situ hybridization, and imaging. The book is a timely and complementary effort to the i5K initiative, which will obtain whole-genome sequences for 5,000 insect and related arthropod species. This comprehensive resource provides cytogeneticists with the necessary background and protocols to understand and develop chromosome-based genome assemblies from such whole-genome sequence data.

Table of Contents

Tephritid Fruit Flies (Diptera); Penelope Mavragani-Tsipidou, Antigone Zacharopoulou, Elena Drosopoulou, Antonios A. Augustinos, Kostas Bourtzis, and Frantisek Marec

Hessian Flies (Diptera); Jeff J. Stuart, Rajat Aggarwal, and Brandon J. Schemerhorn

Tsetse Flies (Diptera); Anna R. Malacrida, Francesca Scolari, Marco Falchetto, and Serap Aksoy

Mosquitoes (Diptera); Maria V. Sharakhova, Phillip George, Vladimir Timoshevskiy, Atashi Sharma, Ashley Peery, and Igor V. Sharakhov

Beetles (Coleoptera); Diogo C. Cabral-de-Mello

Silk Moths (Lepidoptera); Atsuo Yoshido, Ken Sahara, and Yuji Yasukochi

Parasitoid Wasps (Hymenoptera); Jürgen Gadau, Karsten Rütten, and Michaela Neusser

Bedbugs (Hemiptera); Snejana Grozeva, Boris A. Anokhin, and Valentina G. Kuznetsova

Aphids (Hemiptera); Mauro Mandrioli and Gian Carlo Manicardi

Spittlebugs (Hemiptera); Valentina G. Kuznetsova, Anna Maryańska-Nadachowska, and Tatyana Karamysheva

Grasshoppers (Orthoptera); Juan Pedro M. Camacho, Josefa Cabrero, Maria Dolores López-León, Diogo C. Cabral-de-Mello, and Francisco J. Ruiz-Ruano

Ticks (Ixodida); Monika Gulia-Nuss, Jason M. Meyer, and Catherine A. Hill


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Igor V. Sharakhov is an associate professor in the Department of Entomology at Virginia Polytechnic and State University. Dr. Sharakhov earned his PhD in genetics from the Institute of Cytology and Genetics in Novosibirsk, Russia. He leads a research program focused on the comparative and evolutionary genomics of arthropods of medical importance. He has also conducted research on the molecular cytogenetics of African malaria mosquitoes at the State University of New York at Buffalo and on the comparative genome mapping of African malaria mosquitoes at the University of Notre Dame. Dr. Sharakhov serves on the editorial boards of Journal of Insect Science (Entomological Society of America), Journal of Visualized Experiments, and PLoS ONE.