Quantitative bioimaging is a broad interdisciplinary field that exploits tools from biology, chemistry, optics, and statistical data analysis for the design and implementation of investigations of biological processes. Instead of adopting the traditional approach of focusing on just one of the component disciplines, this textbook provides a unique introduction to quantitative bioimaging that presents all of the disciplines in an integrated manner. The wide range of topics covered include basic concepts in molecular and cellular biology, relevant aspects of antibody technology, instrumentation and experimental design in fluorescence microscopy, introductory geometrical optics and diffraction theory, and parameter estimation and information theory for the analysis of stochastic data.
- Comprises four parts, the first of which provides an overview of the topics that are developed from fundamental principles to more advanced levels in the other parts.
- Presents in the second part an in-depth introduction to the relevant background in molecular and cellular biology and in physical chemistry, which should be particularly useful for students without a formal background in these subjects.
- Provides in the third part a detailed treatment of microscopy techniques and optics, again starting from basic principles.
- Introduces in the fourth part modern statistical approaches to the determination of parameters of interest from microscopy data, in particular data generated by single molecule microscopy experiments.
- Uses two topics related to protein trafficking (transferrin trafficking and FcRn-mediated antibody trafficking) throughout the text to motivate and illustrate microscopy techniques.
An online appendix providing the background and derivations for various mathematical results presented or used in the text is available at http://www.routledge.com/9781138598980.
Table of Contents
1. Introduction, Then and Now 2. Introduction to Two Problems in Cellular Biology 3. Basics of Microscopy Techniques 4. Introduction to Image Formation and Analysis 5. From genes to proteins 6. Antibodies 7. Cloning of genes for protein expression 8. Principles of Fluorescence 9. Cells 10. Microscope Designs 11. Microscopy Experiments 12. Detectors 13. Geometrical Optics 14. Diffraction 15. From Photons to Image: Data Models 16. Parameter Estimation 17. Fisher Information and Cram□er-Rao Lower Bound 18.Localizing Objects and Single Molecules in Two Dimensions 19. Localizing Objects and Single Molecules in Three Dimensions 20. Resolution 21. Deconvolution 22. Spatial Statistics. Online Appendices.
Raimund J. Ober received the Ph.D. degree in engineering from Cambridge University, Cambridge, U.K., in 1987. From 1987 to 1990, he was a Research Fellow at Girton College and the Engineering Department, Cambridge University. In 1990, he joined the University of Texas at Dallas, Richardson, where he is currently a Professor with the Department of Electrical Engineering. He is also Adjunct Professor at the University of Texas Southwestern Medical Center, Dallas. He is an Associate Editor of ‘Multidimensional Systems and Signal Processing’ and ‘Mathematics of Control, Signals, and Systems’, and a past Associate Editor of ‘IEEE Transactions on Circuits and Systems’ and ‘Systems and Control Letters’. His research interests include the development of microscopy techniques for cellular investigations, in particular at the single molecule level, the study of cellular trafficking pathways using microscopy investigations, and signal/image processing of bioengineering data.
E. Sally Ward received the Ph.D. degree from the Department of Biochemistry, Cambridge University, Cambridge, U.K., in 1985. From 1985 to 1987, she was a Research Fellow at Gonville and Caius College while working at the Department of Biochemistry, Cambridge University. From 1988 to 1990, she held the Stanley Elmore Senior Research Fellowship at Sidney Sussex College and carried out research at the MRC Laboratory of Molecular Biology, Cambridge. In 1990, she joined the University of Texas Southwestern Medical Center, Dallas, as an Assistant Professor. Since 2002, she has been a Professor in the Department of Immunology at the same institution, and currently holds the Paul and Betty Meek-FINA Professorship in Molecular Immunology. Her research interests include antibody engineering, molecular mechanisms that lead to autoimmune disease, questions related to the in vivo dynamics of antibodies, and the use of microscopy techniques for the study of antibody trafficking in cells.