RNA Nanotechnology and Therapeutics  book cover
1st Edition

RNA Nanotechnology and Therapeutics

ISBN 9781466505667
Published July 9, 2013 by CRC Press
612 Pages 146 B/W Illustrations

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Book Description

Interest in RNA nanotechnology has increased in recent years as recognition of its potential for applications in nanomedicine has grown. Edited by the world's foremost experts in nanomedicine, this comprehensive, state-of-the-art reference details the latest research developments and challenges in the biophysical and single molecule approaches in RNA nanotechnology. In addition, the text also provides in-depth discussions of RNA structure for nanoparticle construction, RNA computation and modeling, single molecule imaging of RNA, RNA nanoparticle assembly, RNA nanoparticles in therapeutics, RNA chemistry for nanoparticle synthesis, and conjugation and labeling.

Table of Contents

Section I Introduction: Principles and Fundamentals of RNA Nanotechnology

The Emerging Field of RNA Nanotechnology

Peixuan Guo

Uniqueness, Advantages, Challenges, Solutions, and Perspectives in Therapeutics Applying RNA Nanotechnology

Peixuan Guo, Farzin Haque, Brent Hallahan, Randall Reif, Hui Li, and Shaoying Wang

Section II RNA Folding, Structure, and Motifs in RNA Nanoparticle Assembly

Kink Turn Structural Motif in RNA

Peter Daldrop, Lin Huang, Kersten T. Schroeder, Jia Wang, and David M. J. Lilley

RNA Nanotechnology: Learning from Biologically Active RNA Nanomachines

Neocles B. Leontis and Emil F. Khisamutdinov

Natural Selection and Structural Polymorphism of RNA 3D Structures Involving GNRA Loops and Their Receptor Motifs

Takahiro Tanaka, Hiroyuki Furuta, and Yoshiya Ikawa

RNA Junction Motifs as Scaffolds for Construction of Multifunctional RNA Nanoparticles

Farzin Haque and Peixuan Guo

Section III RNA Computation and Structure Prediction for RNA Nanoparticle Construction

Role of Dynamics in RNA Nanostructure Design

Wojciech Kasprzak and Bruce A. Shapiro

RNA Three-Dimensional Structure Determination Using Experimental Constraints

Feng Ding and Nikolay V. Dokholyan

Section IV RNA Chemistry for Nanoparticle Synthesis, Conjugation, and Labeling

Nucleotide Chemistry for RNA Nanoparticle Labeling, Conjugation, and Synthesis

Brian M. Laing and Donald E. Bergstrom

RNA Conjugations and Ligations for RNA Nanotechnology

Eduardo Paredes and Subha R. Das

Atom-Specific Mutagenesis of RNAs for Structure, Function, and Therapeutics Studies

Huiyan Sun and Zhen Huang

Section V Single-Molecule and Biophysical Techniques in RNA Nanostructure Analysis

Atomic Force Microscopy of RNA: Imaging and Beyond

Peter M. Schön, Luda S. Shlyakhtenko, and Yuri L. Lyubchenko

Single-Molecule Approach to Study RNA Nanoparticles

Hui Zhang, Chris Richards, Zhengyi Zhao, and Peixuan Guo

Section VI Methods for the Assembly of RNA Nanoparticles

Fabrication Methods for RNA Nanoparticle Assembly Based on Bacteriophage phi29 Packaging RNA Structural Features

Yi Shu, Bahar Seremi, and Peixuan Guo

Synthetic RNA–Protein Nanostructures and Their Potential Applications

Hirohisa Ohno, Eriko Osada, Tan Inoue, and Hirohide Saito

DNA Nanotechnology as Reference for RNA Nanotechnology

Zhen-Gang Wang and Baoquan Ding

Section VII RNA Nanoparticles for Therapy of Cancer, Viral Infections, and Genetic Diseases

Thermodynamically Stable RNA Three-Way Junction for Constructing Multifunctional Nanoparticles for Delivery of Therapeutics

Dan Shu, Yi Shu, Farzin Haque, Sherine Abdelmawla, and Peixuan Guo

Design and Construction of RNA Nanoparticles Targeting Prostate Cancer

Randall Reif and Peixuan Guo

Conjugation of RNA Aptamer to RNA Nanoparticles for Targeted Drug Delivery

Katherine Germer, Fengmei Pi, Peixuan Guo, and Xiaoting Zhang

Extracellular Membrane Vesicles and Extracellular Membrane Vesicle–Based Therapeutics for Brain Diseases

Jayden A. Smith, Clara Alfaro-Cervello, Chiara Cossetti, Nunzio Iraci, Matilde Stefanini, and Stefano Pluchino

Pharmacokinetics and Pharmacodynamics of RNA Nanoparticles

Markos Leggas

Section VIII RNA Nanotechnology for Diagnostic Applications

RNA Nanotechnology in Sensing, Detection, and Disease Diagnosis

Ping Lei and Guanxin Shen

Potentials of RNA Aptamers for Viral Detection and Treatment

Longxin Chen, Runting Li, and Runlin Z. Ma

Section IX Application of RNA Aptamers in RNA Nanotechnology and Therapeutics

Application of RNA Aptamers in Nanotechnology and Therapeutics

Hua Shi

Aptamers Targeting a Subunit or a Conformation of Glutamate Ion Channel Receptors

Zhen Huang, William Jaremko, Chi-yen Lin, and Li Niu

Section X Application of miRNAs in RNA Nanotechnology and Therapeutics

Application of MicroRNAs in RNA Nanotechnology and Antiviral Therapeutics

Ye Qiu, Xin Ye, Maged Hemida, Mary Zhang, Paul Hanson, and Decheng Yang

MicroRNAs: Biology and Role in RNA Nanotechnology

Bin Guo and Daniel W. Binzel

Section XI Application of siRNAs in RNA Nanotechnology and Therapeutics

Current Advances in Self-Assembly RNAi Nanoparticles

Ka-To Shum, Jiehua Zhou, and John J. Rossi

Self-Assembly of siRNA Containing Nanoparticles

Mengyao Zheng, Thomas Kissel, and Olivia M. Merkel


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Peixuan Guo is the Director of the NIH Nanomedicine Development Center at the University of Cincinnati, Ohio and the Director of the NIH/NCI Cancer Nanotechnology Platform Partnership Program: RNA Nanotechnology for Cancer Therapy at Purdue University, Indiana.


"The main strength of this book is in its focus on RNA as a functional agent. RNA can be derivatized using a variety of biochemical technologies to incorporate therapeutic or imaging functionalities. This book spans different aspects of techniques that are necessary to obtain functional RNA nanoparticles, including computational design, synthesis of components, assembly of functional units, and their biochemical and biophysical characterization. It also discusses promises and challenges in the applications of RNA nanoparticles as therapeutics. … This book is unique in that it covers a very broad spectrum of topics related to RNA nanotechnology, yet it maintains a focus on constructing and analyzing RNA nanoparticles. It introduces the reader to the basics of RNA structure, and discusses functions of various natural RNAs, from small molecules to large molecular machines such as the ribosome. It also introduces the concept of designing new RNAs, and provides a comprehensive coverage of modern techniques, linking theory and experiment. Technologies discussed include RNA structure prediction, prediction of interactions, simulations of RNA folding, computational design, chemical methods for RNA nanoparticle synthesis, assembly, and labeling, as well as methods for their biophysical and biochemical characterization and biomolecular imaging. … Chapters 1, 4, 7 are very exciting. Chapter 1 is a condensed introduction to the topic of RNA nanotechnology and to challenges and opportunities in using the RNA molecules as therapeutic agents. It is quite obvious that Dr. Guo is an enthusiast of RNA nanotechnology and in fact, this enthusiasm is quite contagious. Chapters 4 and 7 provide more focused description of two issues: 1) naturally existing RNAs as molecular machines, with a very informative discussion of differences between molecular machines and macro-machines, using ribosome as an example and 2) the role of dynamics (as opposed to 3D structure alone) in RNA design. Both these chapters demonstrate the extraordinary interdisciplinary character of the field of RNA nanotechnology, as well asof this very book. I definitely want to have this book on my shelf, and I intend to read all chapters as soon as I get it!"
––Prof. Janusz M. Bujnicki, International Institute of Molecular and Cell Biology in Warsaw, and Adam Mickiewicz University in Poznań, Poland

"This book is very good in this field. I want to have this book on my shelf. … This book has a broad coverage from principles and fundamentals of RNA nanotechnology, RNA nanotechnology design and construction to diagnostic and therapeutic application. These detailing descriptions provide a clear and complete picture of up-to-date knowledge of RNA nanotechnology and applications."
––Prof. Shu-Feng Zhou, University of South Florida

"The range of topics is excellent. … A nice collection of, basic and focused, articles on RNA."nanotechnology, a fast developing field."
—Eric Westhof, IBMC-CNRS, Université de Strasbourg, France