New Findings Revolutionize Concepts of Gene Function
Endogenous small RNAs have been found in various organisms, including humans, mice, flies, worms, fungi, and bacteria. Furthermore, it’s been shown that microRNAs acting as cellular rheostats have the ability to modulate gene expression. In higher eukaryotes, microRNAs may regulate as much as 50 percent of gene expression.
Regulation of Gene Expression by Small RNAs brings together the pioneering work of researchers who discuss their work involving a wide variety of small RNA regulatory pathways in organisms ranging from bacteria to humans. In addition to exploring the biogenesis and processing of these regulatory RNAs, they also consider the functional importance of these pathways in host organisms. Assisting current and future researchers, this unique groundbreaking work —
- Provides a suite of cutting-edge resources for the study of microRNA ontology and function
- Includes a technology guide for those seeking to assay microRNA expression
- Explores the mechanisms by which microRNAs regulate gene expression in animal cells, including the regulation of gene expression by RNA-mediated transcriptional gene silencing
- Discusses a fast and low-cost approach for reversing genetic influences in mammals
- Looks at breakthroughs in the use of microRNA-based therapy for HIV and cancer
This volume captures the essence of the breadth and excitement surrounding the newly discovered regulatory roles of small RNAs. The powerful new approach in the study of gene function described in this text is leading to some remarkable findings that have the potential to revolutionize our understanding of genetic function and the treatment of diseases otherwise considered intractable.
Table of Contents
MicroMining: Computational Approaches to microRNA Discovery, A. Grundhoff
Animal microRNA Gene Prediction, O. Snøve and P. Sætrom
A Suite of
Resources for the Study of microRNA Ontology and Function, P. Sethupathy, M.
Megraw, and A. G. Hatzigeorgiou
Regulation of Translation and mRNA Stability by Hfq-Binding Small RNAs in Escherichia coli, H. Aiba
Mechanisms by which microRNAs Regulate Gene Expression in Animal Cells, Y. Yu and T. W. Nilsen
The microRNAs of Caenorhabditis elegans, M. J. Nolde and F. J. Slack
Isolation and Characterization of Small RNAs in Caenorhabditis elegans, C. Ushida and Y. Hokii
MicroRNA Tales in Fly Development, U. Bhadra, S. K. Singh, S.N.C.V.L. Pushpavalli, P. B. Hajeri, and M. Pal-Bhadra
RNA Interference and microRNAs in Zebra Fish, A. S. Flynt, E. J. Thatcher, and J. G. Patton
Biogenesis and Function of Plant microRNAs, Z. Havelda
Endogenous Small RNA Pathways in Arabidopsis, M. Agarwal, J. Curaba, and X. Chen
How to Assay microRNA Expression: A Technology Guide, M. Castoldi, V. Benes, and M. U. Muckenthaler
Methods to Quantify microRNA Gene Expression, L. A. Neely
Regulation of Alternative Splicing by microRNAs, R. K. Gaur
Recent Progress in Polymerase II-Mediated Intronic microRNA Expression Systems, S.-L. Lin and S.-Y. Ying
MicroRNA-Based RNA Polymerase II Expression Vectors for RNA Interference in Mammalian Cells, A. B. Vojtek, K.-H. Chung, P. D. Patel, and D. L. Turner
A Fast and Low-Cost Approach to Reverse Genetics in Mammals, L. Qiu and Z.
Symphony of AIDS: A microRNA-Based Therapy, Y. R. Fujii
Cancer: Connecting the Dots, S. D. Jayasena
Mammalian Transcriptional Gene Silencing by Small RNAs, D. H. Kim and J. J. Rossi
Regulation of Gene Expression by RNA-Mediated Transcriptional Gene Silencing, K. V. Morris