Revival: Procedure Manual for the Diagnosis of Intestinal Parasites (1994): 1st Edition (Paperback) book cover

Revival: Procedure Manual for the Diagnosis of Intestinal Parasites (1994)

1st Edition

By Donald L. Price

CRC Press

286 pages

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Description

Procedure Manual for the Diagnosis of Intestinal Parasites is the definitive resource for individuals involved in the collection, preparation, and examination of fecal specimens for microscopic diagnosis of intestinal parasitic infections. The book points out the stages of parasites possibly found in a fecal specimen, how to find them, and how to identify them. Specific details on how to effectively use the microscope for parasitic diagnosis are included. This information is missing from most texts and manuals of this kind.

Photomicrographs and original drawings of the various stages and forms of parasites and eggs are used extensively throughout the text, with the photomicrographs printed to a standard scale for easy comparison. More than 400 illustrations in all are included. Biological keys for intestinal amoebae and eggs of various species of helminths are provided.

This book is an essential reference for teachers of diagnostic parasitology and their students, physicians who order fecal examinations for intestinal parasites, nurses or health workers who handle or prepare the specimens for the laboratory, and technologists who receive, process, and examine the specimens.

Table of Contents

Introduction

Survival of Trophozoites and Cysts in Unpreserved Fecal Specimens

Collecting/Preserving Solutions

The Use of Collecting/Preserving Solutions

The Quality of Preserved Eggs and Parasites

Background of the MIF-Type Collecting/Preserving Solutions

Environmental Considerations

Application of Collecting/Preserving Methods

The Specimen

Fecal Specimens in the Clinical Laboratory

Factors Having an Impacting on Specimen Collection

Procedure for Specimen Collection for Parasitology

Factors Having an Impact on Specimen Preparation

Written Instructions for Specimen Collection.

Instructions for Collecting Fecal Specimens

The Microscope and Examination of the Specimen

The Microscope and Microscopy

Using Their Refractive Index to Find Parasites and Eggs

Eyepiece Micrometer

Selection of Lenses

Preparing Wet-Mount Slides from the Preserved Specimen for Microscopic Examination

Concentration Methods

Introduction

Use of the Centrifuge for Concentration Methods

Flotation Methods

Sedimentation Methods

Stains and Staining Methods

Stains and Staining Methods

Preparing Permanently Stained Fecal Films

Preparing Fecal Films for Permanent Staining

Staining Methods for Preparing Permanently Stained Fecal Films from Specimens Fixed in PVA

Staining Methods for Preparing Permanently Stained Fecal Films from Specimens Fixed in SAF, PIF, or MIF

Reusing and Changing Solutions in the Staining Setup

Application of Methods

Comparison of Collecting/Preserving Solutions.

Selecting Methods for the Laboratory

Special Methods

Acid-Fast Stains

Preparing Temporary Wet Mounts

Egg Count Procedures

Post-Treatment Examinations

Introduction to the Parasites

Parasites Identified by Examining Fecal Preparations

Helminth Parasites in General

Protozoan Parasites in General

Source of Infection and Modes of Transmission

The Helminths

Diagnosis of Helminth Infections

Trematode Eggs

Cestode Eggs

Nematode Eggs

Nematode Juveniles

Introduction to the "Key to Helminth Eggs Found in Feces"

Photomicrographs of Helminth Eggs

Key to Helminth Eggs Found in Feces

The Protozoa

Structure of the Intestinal Protozoa

Structure of the Intestinal Amoebae

Structure of the Intestinal Flagellates

Structure of the Intestinal Ciliates

Structure of the Intestinal Coccidia

The Intestinal Amoebae

Introduction to the "Key for Differentiating Species of Intestinal Amoebae in Fecal Specimens Preserved in PIF or MIF"

Key for Differentiating Species of Intestinal Amoebae in Specimens Preserved in PIF or MIF

The Intestinal Flagellates

The Intestinal Ciliates

The Intestinal Coccidia

Protozoa of Uncertain Taxonomic Position

Photomicropgraphs of Intestinal Protozoa

Nonhuman Parasites and Structures that Mimic Parasites

Structures Resembling Protozoa

Structures Resembling Helminth Eggs

Spurious Infections with Eggs and Cysts of Animals

Appendices

Technical Information

Glossary

References

Index

About the Author

Donald L. Price, Ph.D., received his doctorate in zoology from the University of Maryland, with a specialty in Medical Zoology. He has broad experience as a scientist, consultant, and educator. For 22 years, Dr. Price served in the United States Army, first as a pilot in WWII, and for 18 years as a Medical Zoologist (parasitology) in the Medical Service Corps. He retired in 1968 as a Lieutenant Colonel.

About the Series

CRC Press Revivals

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Subject Categories

BISAC Subject Codes/Headings:
MED067000
MEDICAL / Pathology
SCI045000
SCIENCE / Life Sciences / Biology / Microbiology