1st Edition

Revival: Spheroid Culture in Cancer Research (1991)





ISBN 9781138561908
Published January 28, 2019 by CRC Press
344 Pages

USD $68.95

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Book Description

Spheroid Culture in Cancer Research describes the various techniques now available for establishing spheroid tissue culture, including spheroid culture from normal tissues and from tumor cell lines. The book also describes how the spheroid system can be used to study interactions between normal and malignant cells. Microenvironmental conditions in spheroids and how this micromilieu may promote cellular heterogeneity and histiotypic structures not observed in corresponding monolayer cultures are discussed. The biological importance of oxygen tension, pH gradients, diffusions of nutrients, and cell-cell communication in spheroids are also examined.

The book will be profoundly important to researchers in experimental chemotherapy, radiotherapy, immunotherapy, and hyperthermia.

Table of Contents

METHODS OF SPHEROID TISSUE CULTURE. TUMOR SPHEROIDS FROM MONOLAYER CULTURES (Morten Lund-Johansen, Rolf Bjerkvig and Garry J. Rucklidge). SPHEROIDS OF NORMAL TISSUE IN CULTURE (Sverre Karmhus Steinsvaag and Robert Bjerknes). TUMOR SPHEROIDS FROM BIOPSY SPECIMENS (Rolf Bjerkvig, Jens Høstmark, Paal Henning Pederson and Ole Didrik Laerum). TUMOR CELL INVASION IN VITRO. MECHANISMS OF TUMOR CELL INVASION STUDIED IN VITRO (Michael E. Berens). THE USE OF SPHEROIDS IN THE STUDY OF INVASION (Marc Bracke, Hans Romijn, Luc Vakaet Jr., Barbara Vyncke, Marc De Mets and Marc Mareel). TUMOR CELL INVASION IN VITRO IN SYNGENEIC AND HETEROLOGOUS SYSTEMS (Olav Engebraaten). CELL INTERACTIONS IN THREE-DIMENSIONAL CULTURE. THE MICROENVIRONMENT IN MULTICELLULAR SPHEROIDS (Helmut Acker and Jörgen Carlsson). CELL DIFFERENTIATION AND HETEROGENEITY IN SPHEROID CULTURE (Ruth Knüchel and Robert M. Sutherland). INTERCELLULAR COMMUNICATION IN THREE-DIMENSIONAL CULTURE (Dieter F. Hülser). SPHEROIDS IN EXPERIMENTAL THERAPY. TUMOR SPHEROIDS AS A MODEL IN STUDIES OF DRUG EFFECTS (Jörgen Carlsson and Thore Nederman). SPHEROIDS IN RADIOBIOLOGY RESEARCH (James P. Freyer). TUMOR SPHEROIDS IN STUDIES OF IMMUNOTHERAPY (Jörgen Carlsson). USE OF SPHEROIDS IN HYPERTHERMIA RESEARCH (Baard-Christian Schem and Olav Dahl).

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Author(s)

Biography

Rolf Bjerkvig, Ph.D., is appointed as a Research Scientist at the Norwegian Cancer Society, and as a Senior Lecturer at the Gade Institute, Department of Pathology, University of Bergen, Norway.