Second-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissues, with applications in basic science, medical research, and tissue engineering. Second Harmonic Generation Imaging offers a complete guide to this optical modality, from basic principles, instrumentation, methods, and image analysis to biomedical a
Principles of SHG Imaging: Theoretical Framework for SHG Microscopy: A Matrix-Based Approach. How to Build an SHG Apparatus. THG Microscopy of Cells and Tissues: Contrast Mechanisms and Applications. SHG Microscopy and Its Comparison with THG, CARS, and Multiphoton Excited Fluorescence Imaging. Biomedical Imaging Using SHG: Molecular Structure and Order with Second-Harmonic Generation Microscopy. 3D SHG Imaging and Analysis of Fibrillar Collagen Organization. Second-Harmonic Generation Imaging of Microtubules. SHG and Optical Clearing. Holographic SHG Imaging. Imaging Membrane Potential with SHG. Applications of SHG: Second-Harmonic Generation Imaging of Self-Assembled Collagen Gels. SHG and Multiphoton Fluorescence Imaging of the Eye. Multiphoton Excitation Imaging of the Arterial Vascular Bed. Combined SHG/THG Imaging. SHG Imaging of Collagen and Application to Fibrosis Quantization. Quantitative Approaches for Studying the Role of Collagen in Breast Cancer Invasion and Progression. SHG in Tumors: Scattering and Polarization. SHG Imaging for Tissue Engineering Applications. Index.