560 pages | 107 Color Illus. | 50 B/W Illus.
This is a comprehensive guide to single-stranded RNA phages (family Leviviridae), first discovered in 1961. These phages played a unique role in early studies of molecular biology, the genetic code, translation, replication, suppression of mutations. Special attention will be devoted to modern applications of the RNA phages and their products in nanotechnology, vaccinology, gene discovery, evolutionary and environmental studies. This overview of the generation of novel vaccines, gene therapy vectors, drug delivery and diagnostic tools will also explore the role of RNA phage-derived products in the revolutionary progress of the protein tethering and bioimaging protocols will be outlined.
1. History and general properties.
3. Male specificity, hosts, and natural distribution.
4. Cultivation and preparative biotechnological aspects.
6. Genome organization.
7. Structure of the phage RNA.
8. Genetic studies of RNA phages.
9. RNA phage proteins: coat protein.
10. RNA phage proteins: A protein.
11. RNA phage proteins: replicase protein.
12. RNA phage proteins: lysis protein.
13. Spatial structure of RNA phage particles.
14. Antigenicity and immunogenicity of RNA phages.
15. Translation and genetic code.
17. Evolutionary experiments.
18. Homologous and heterologous expression of the RNA phage genes.
19. Chimeric virus-like particles.
20. RNA phage VLP-based vaccines.
21. Nonvaccine VLP applications.
22. Non-VLP applications.
23. Environmental applications: water, food, biomedical safety.
24. General conclusions and future perspectives.