Synthetic Biology Handbook: 1st Edition (Hardback) book cover

Synthetic Biology Handbook

1st Edition

Edited by Darren N. Nesbeth

CRC Press

318 pages | 32 Color Illus. | 54 B/W Illus.

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pub: 2016-04-13
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Description

The Synthetic Biology Handbook explains the major goals of the field of synthetic biology and presents the technical details of the latest advances made in achieving those goals. Offering a comprehensive overview of the current areas of focus in synthetic biology, this handbook:

  • Explores the standardisation of classic molecular bioscience approaches
  • Addresses the societal context and potential impacts of synthetic biology
  • Discusses the use of legacy systems as tools for new product development
  • Examines the design and construction of de novo cells and genetic codes
  • Describes computational methods for designing genes and gene networks

Thus, the Synthetic Biology Handbook provides an accurate sense of the scope of synthetic biology today. The handbook also affords readers with an opportunity to scrutinize the underlying science and decide for themselves what aspects of synthetic biology are most valuable to their research and practice.

Table of Contents

STANDARDISING BIOLOGY

Synthetic Biology: Culture and Bioethical Considerations

Introduction

Perceptions and Portrayals of Synthetic Biology in Popular Culture and Politics

Synthetic Biology in Academic Literature

Societal Risks and Responsibilities Posed by Synthetic Biology

Governance of Synthetic Biology

Conclusions

Acknowledgements

References

Synthetic Biology Standards and Methods of DNA Assembly

Introduction

Theory and Considerations in Double-Stranded DNA Assembly

Assembly Methods in Detail

Distributed Construct Assembly Methods

Building Combinatorial Complexity during Assembly

Emerging Technologies and Conclusions

Standardisation of DNA Assembly

References

Standardised Genetic Output Measurement

Introduction

Standardisation in Conventional Biology

Standardisation in Synthetic Biology

Adapting Current Techniques as Synthetic Biology Standards

Towards Synthetic Biology Standards for Protein Measurement

Conclusions

Acknowledgements

References

ENGINEERING BIOLOGY WITH LEGACY TOOLS

Bacterial Cells as Engineered Chassis

Introduction

Designing and Refactoring Bacterial Genes

Bacterial Synthetic Gene Networks

Genome-Scale Re-Engineering of Bacterial Cells

Engineering Periplasmic Space

Re-Imagining the Bacterial Surface and Glycocalyx

References

Eukaryotae Synthetica: Synthetic Biology in Yeast, Microalgae, and Mammalian Cells

Introduction

Remodelling Outer Cell Membranes as Engineered Chassis Surfaces

Programmable Organelles

Eukaryotic Genes and Their Synthetic Counterparts

Networks of Synthetic Genes in Mammalian Cells

Genome-Scale Engineering in Eukaryotes

Synthetic Biotherapeutics

References

Synthetic Plants

Tools and Resources

Food Security and Sustainable Agriculture

Plant Chassis Engineering to Improve Nutritive Value

Synthetic Biology and Green Biopharms

Fibre and Fuel

Future of Plant Synthetic Biology

References

CONSTRUCTING NEW BIOLOGIES

Theory and Construction of Semi-Synthetic Minimal Cells

Introduction

Autopoiesis and Minimal Life

Semi-Synthetic Approach to the Construction of Minimal Cells

Entrapment of Solutes inside Liposomes

Biotechnological Perspectives of Synthetic Cells

Concluding Remarks

Acknowledgements

References

Design Tools for Synthetic Biology

Introduction

Mathematical Modelling in Synthetic Biology

Part Registries

Device Languages

Modelling Languages and Tools

Network Optimisation

Part Engineering

DNA Editing Tools

CAD Tools and Workflow Systems

Summary

References

New Genetic Codes

Introduction

Available Orthogonal Pairs and Their Host Systems

Using UAAs to Encode Post-Translational Modifications

UAAs as Synthetic Biology Tools to Detect Interactions Occurring within Cells

Photo-Activatable UAAs

Bioorthogonal Chemistry

Orthogonal Ribosomes

Systemic Optimization of New Genetic Codes

References

About the Editor

Darren N. Nesbeth earned his BSc in molecular biology from University College London (UCL), UK, and his PhD in molecular cell biology from Imperial College, London, UK. He did postdoctoral work on scale up of gene therapy at King's College London, UK; fundamental cell biology at Imperial College; and industrially applied cell engineering at UCL. In the UCL Department of Biochemical Engineering, he applies synthetic biology to address industrial challenges in manufacturing of biopharmaceuticals and fine chemicals.

Subject Categories

BISAC Subject Codes/Headings:
MED009000
MEDICAL / Biotechnology
SCI010000
SCIENCE / Biotechnology