1st Edition

TRP Channels

Edited By Michael X. Zhu Copyright 2011
    500 Pages 7 Color & 49 B/W Illustrations
    by CRC Press

    500 Pages 7 Color & 49 B/W Illustrations
    by CRC Press

    The rapid expansion of the TRP field has generated a large amount of excellent original work across many different research fields. However, investigators are not necessarily familiar with the pros and cons of the variety of methods used to study TRP channels. Because of functional and genetic diversity, as well as the different physiological roles they play, techniques used for studying TRP channels range from single molecular analysis to behavioral animal studies. Methods in multiple areas, such as molecular biology, fluorescence imaging, electrophysiology, cell biology, genetics, proteomics, pharmacology, system physiology, and behavioral assessment, are employed to investigate various aspects of these channels. Choosing among many possible topics in these broad areas was a daunting task.

    A comprehensive review of the field, TRP Channels spans the information gap by providing broad coverage of current methods and techniques commonly used in TRP channel research, and detailed protocols with thorough discussions of the advantages and disadvantages across methods. Some topics covered include

    • Mammalian, Drosophila and C. elegans TRP channels
    • Practical protocols for functional studies of TRP channels, including TRPC, TRPV, TRPA, TRPM and the intracellularly localized TRPML channels
    • ThermoTRPs, including the new fast temperature jump apparatus and the high throughput random mutagenesis method for screening critical motifs involved in TRP channel regulation
    • Cell-based high-throughput screening assays for TRP channels and their applications in drug discoveries
    • TRP channel functions in native cells, including smooth muscles, neurons, and cancers

    Showcasing the current status of the field, TRP Channels covers the major techniques used in various areas of research. The majority of the chapters are protocol oriented, with the goal of providing clear directions for laboratory use. Because of the breadth of the TRP field, the applications of some methods are described in multiple chapters by experts working on a variety of channel types that serve different physiological functions, highlighting distinctive views on how the methodology can be utilized. Some chapters include discussion on the usefulness and pitfalls associated with the use of certain techniques. Together with chapters that offer comprehensive reviews on the functional regulation and other roles of TRP channels, students and investigators new to the field should find this book particularly informative.

    High-Throughput Screening of TRPC Channel Ligands Using Cell-Based Assays
    Melissa Miller, Meng Wu, Jia Xu, David Weaver, Min Li, and Michael X. Zhu

    Methods to Study TRPC Channel Regulation by Interacting Proteins
    Kirill Kiselyov, Kyu Pil Lee, Joseph P. Yuan, and Shmuel Muallem

    Activation of TRP Channels in Mammalian Systems
    Tomohiro Numata, Shigeki Kiyonaka, Kenta Kato,Nobuaki Takahashi, and Yasuo Mori

    Studying Subunit Interaction and Complex Assembly of TRP Channels
    Michael Poteser and Klaus Groschner

    Proteomic Analysis of TRPC Channels
    Timothy Lockwich, Anthony Makusky, Jeffrey A. Kowalak, Sanford P. Markey, and Indu S. Ambudkar

    Lessons of Studying TRP Channels with Antibodies
    Marcel Meissner, Verena C. Obmann, Michael Hoschke, Sabine Link, Martin Jung, Gerhard Held, Stephan E. Philipp, Richard Zimmermann, and Veit Flockerzi

    Assessing TRPC Channel Function Using Pore-Blocking Antibodies
    Shang-Zhong Xu

    Strategies and Protocols to Generate Mouse Models with Targeted Mutations to Analyze TRP Channel Functions
    Marc Freichel, Ulrich Kriebs, Dominik Vogt, Stefanie Mannebach, and Petra Weißgerber

    Studying Endogenous TRP Channels in Visceral and Vascular Smooth Muscles
    Alexander V. Zholos

    TRPC Channels in Neuronal Survival
    Junbo Huang, Wanlu Du, Hailan Yao, and Yizheng Wang

    Electrophysiological Methods for the Study of TRP Channels
    Karel Talavera and Bernd Nilius

    High-Throughput Approaches to Study Mechanisms of TRP Channel Activation
    Hongzhen Hu, Michael Bandell, Jorg Grandl, and Matt Petrus

    Methods Used for Studying TRP Channel Functions in Sensory Neurons
    Louis S. Premkumar

    Time-Resolved Activation of Thermal TRP Channels by Fast Temperature Jumps
    Feng Qin

    Fluorescence Microscopy in Thermo TRP Channel Research
    Fan Yang and Jie Zheng

    Regulation of TRP Channels by Osmomechanical Stress
    Min Jin, Jonathan Berrout, and Roger G. O’Neil

    Study of TRP Channels in Cancer Cells
    V’yacheslav Lehen’kyi and Natalia Prevarskaya

    In Vitro and In Vivo Assays for the Discovery of Analgesic
    Drugs Targeting TRP Channels
    Jun Chen, Regina M. Reilly, Philip R. Kym, and Shailen Joshi

    Studying TRP Channels in Intracellular Membranes
    Mohammad Samie and Haoxing Xu

    Methods for Studying Drosophila TRP Channels
    Elkana Kohn and Baruch Minke

    Studying TRP Channels in Caenorhabditis elegans
    Rui Xiao and X.Z. Shawn Xu


    Michael Xi Zhu is currently with the Department of Integrative Biology and Pharmacology at the University of Texas Health Science Center at Houston