5th Edition

Textbook of Assisted Reproductive Techniques
Volume 1: Laboratory Perspectives

ISBN 9781498740142
Published January 22, 2018 by CRC Press
467 Pages 69 Color & 74 B/W Illustrations

USD $220.00

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Book Description

Established as a definitive reference for the IVF clinic, the fifth edition has been extensively revised, with the addition of several important new contributions on laboratory topics, including advanced sperm selection techniques for ICSI, human embryo biopsy procedures, oocyte activation, managing an oocyte bank, artificial gametes, and epigenetics. As previously, methods, protocols, and techniques of choice are presented by IVF pioneers and eminent international experts.

Table of Contents

Contents. List of contributors.The beginnings of human in vitro fertilization. Robert Edwards and the thorny path to the birth of Louise Brown: A history of IVF and ET. New guidelines for setting up an ART laboratory. Quality control: Maintaining stability in the laboratory. The ART laboratory: Current standards. Evaluation of sperm. Sperm preparation techniques. Sperm chromatin assessment. Oocyte retrieval and selection. Preparation and evaluation of oocytes for ICSI. Advanced sperm selection techniques for ICSI. Use of In Vitro Maturation in a clinical setting: Patient populations and outcomes. Intracytoplasmic sperm injection: Technical aspects. Assisted hatching. Human embryo biopsy procedures. Assisted oocyte activation (AOA): Current understanding, practice, and future perspectives. Analysis of fertilization. Culture systems for the human embryo. Evaluation of embryo quality: Analysis of morphology and physiology. Evaluation of embryo quality: Time-lapse imaging to assess embryo morphokinesis. Evaluation of embryo quality: Proteomic strategies. The human oocyte: Controlled rate cooling. The human oocyte: Vitrification. The human embryo: Slow freezing. The human embryo: Vitrifiation. Managing an oocyte bank. Severe male factor: Genetic consequences and recommendations for genetic testing. Polar body biopsy and its clinical application. Pre-implantation genetic diagnosis for infertility. Genetic analysis of the embryo. Diagnosis of endometrial receptivity and the embryo-endometrial dialogue. Artificial gametes: the oocyte. Microfluidics in ART: Towards automation of the IVF laboratory. Epigenetic considerations in preimplantation mammalian embryos. Index

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David K. Gardner DPhil
Chair of Zoology, University of Melbourne, Melbourne, Australia

Ariel Weissman MD
Senior Physician, IVF Unit, Department of Obstetrics and Gynecology, Edith Wolfson Medical Center, Holon and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel

Colin M. Howles PhD, FRSM
Aries Consulting SARL, Geneva, Switzerland
Honorary Fellow, University of Edinburgh, UK

Zeev Shoham MD
Director, Reproductive Medicine and Infertility Unit, Department of Obstetrics and Gynecology, Kaplan Medical Center, Rehovot, Israel