Understanding the diagnostic methods necessary to identify bloodstream infections.
In the clinical microbiology laboratory, blood is a critical diagnostic sample that, in the majority of cases is sterile (or is it?). However, when microbes gain access to and multiply in the bloodstream, it can result in life-threatening illness including sepsis. Mortality rates from bloodstream infection and sepsis range from 25% to 80%, killing millions of people annually. Blood cultures are a vital technology used in the microbiology laboratory to isolate and identify microbes and predict their response to antimicrobial therapy.
The Dark Art of Blood Cultures, edited by Wm. Michael Dunne, Jr., and Carey-Ann D. Burnham, surveys the entire field of blood culture technology, providing valuable information about every phase of the process, from drawing samples to culture methods to processing positive cultures. The Dark Art of Blood Cultures is organized around several major topics.
1: Perspectives on the Art and Science of Blood Culture
1. Historical Perspectives on the Art and Science of Blood Culture
William Michael Dunne, Jr.
2. Conventional Blood Culture Methods
Robyn Atkinson-Dunn and William Michael Dunne, Jr.
3. Lysis-Centrifugation Methods of Blood Culture
Erin McElvania TeKippe and Morgan A. Pence
4. BACTEC Blood Culture Systems
Robin R. Chamberland
2: Modern Methods in Blood Culture
5. The BioMérieux BacT/ALERT: Automation at Last in the Black Box
Bradley Ford and George Kallstrom
6. TREK Blood Culture Systems
Neil W. Anderson and Melanie Yarbrough
7. Molecular Methods for Detection of Pathogens Directly from Blood Specimens
Mark D. Gonzalez and Robert C. Jerris
8. Pediatric Blood Cultures
Paula Revell and Christopher Doern
9. Epidemiology of Bloodstream Infections
Allison R. McMullen, Craig B. Wilen, and Carey-Ann D. Burnham
10. Best Practices in Blood Cultures
Robert J. Tibbetts and Barbara Robinson-Dunn
11. Processing Positive Cultures
Matthew L. Faron and Nathan A. Ledeboer
12. Fungal and Mycobacterial Blood Cultures
Robert S. Liao and William Lainhart
4: New Developments in Blood Culture
13. The Bacterial Blood Microbiota/Microbiome
Eileen M. Burd and Lars F. Westblade