The Platelet Amine Storage Granule presents a comprehensive examination of the dense granule of human platelets and the development of dense granule precursors in megakaryocytes. Fundamental aspects addressed in the book include a description of the amine-nucleotide storage complex, the process of granule secretion (with focus on inositol phosphate metabolism), modulation of secretion by epinephrine, and a description of platelet storage pool disease. The book's broad coverage of the topic makes it a valuable resource to hematologists, cell biologists, platelet biologists, medical and veterinary geneticists, neurobiologists, endocrinologists, physiologists, and pharmacologists.
About the Editors. Contributors. Preface. THE DENSE BODIES OF HUMAN PLATELETS (James G. White). Discovery and Characterization of Dense Bodies. The Number of Dense Bodies in Human Platelets. Origin of Platelet Dense Bodies. Secretion of Platelet Dense Bodies. Inherited Disorders of Dense Bodies. Summary. ULTRASTRUCTURE OF DENSE GRANULE PRECURSOR DEVELOPMENT IN MEGAKARYOCYTES (Michéle Ménard and Kenneth M. Meyers). MK Classification with Transmission Electron Microscopy. Dense Granule Precursors in MK with Standard Processing for Transmission Electron Microscopy. Uranaffin Cytochemical Reaction. Fixation in a Calcium-Supplemented Fixative. Studies with Extracellular Electron Dense Markers. Dense Granule Precursors in MK from Animal Models of the Chediak-Higashi Syndrome (CHS). List of Abbreviations. NUCLEAR MAGNETIC RESONANCE STUDIES OF AMINE AND NUCLEOTIDE STORAGE MECHANISMS IN PLATELET DENSE GRANULES (Holm Holmsen and Kamil Ugurbil). NMR Measurements: Some Basic Considerations. Compartmentation of Nucleotides in Platelets. of Dense Granules in Human and Porcine Platelets. Use of 31P-NMR to Study Nucleotides in Intact. 1H-NMR of Porcine Platelet Dense Granule Constituents. Conclusions. Acknowledgments. THE ROLE OF INOSITOL PHOSPHATE METABOLISM IN PLATELET STIMULUS-RESPONSE COUPLING (James L. Daniel). Inositol Phosphate Metabolism and Platelet Activation. The Metabolism of Ins 1,4,5-P3. Concluding Remarks. Acknowledgments. POLYPHOSPHOINOSITIDE METABOLISM IN RESTING AND STIMULATED PLATELETS (Holm Holmsen, Ole-Bjørn Tysnes and Adrie J.M. Verhoeven). Platelet Activation. Polyphosphoinositide (PPI) Cycle and Radioisotopic Labeling. Time Courses of Changes in 32P-PPIs During Platelet Stimulation. Turnover of PI in Resting and Stimulated Platelets. Turnover of PIP and PIP2 in Resting and Stimulated Platelets. Rapid Influx and Efflux of Inorganic Phosphate During Platelet Stimulation. Rates of Production and Consumption of PA in Stimulated Platelets-The Existence of a Non-Equilibrium Condition. Metabolic Heterogeniety of PA in Resting and Stimulated Platelets. Conversion Rates in the Individual Steps in the PPI Cycle of Resting and Stimulated Platelets-Purpose of the High Turnover Rates of the PPI Monoesters. Acknowledgments. List of Abbreviations. EPINEPHRINE-INDUCED PLATELET MEMBRANE MODULATION (Gundu H.R. Rao and James G. White). Facilitating Influence of Epinephrine on Platelet Function. Alpha Adrenoceptors and Platelet Function. Discovery of Platelet Membrane Modulation. Irreversible Aggregation Independent of the Mediation by Cyclooxygenase Products or Dense Granule . Mechanism Involved in Epinephrine-Induced Restoration of Function in Aspirin-Treated Platelets. Studies on Membrane Modulation in Various Platelet Disorders. Epinephrine-Induced Restoration of Function in Drug-Induced Refractory Platelets. Is Membrane Modulation Different from Synergism? Mechanisms Involved in Epinephrine-Induced Facilitation of Platelet Function. Mechanisms Involved in Epinephrine-Induced Membrane Modulation in Drug-Induced Refractory Platelets. Clinical Significance of Membrane Modulation. Summary. Acknowledgments. List of Abbreviations. PLATELET STORAGE POOL DEFICIENCY (Kenneth M. Meyers and Michéle Ménard). Congenital SPD. Acquired SPD. Treatment. Summary. Index.