This fascinating book discusses various methods that have been used from early times to the present for the isolation and characterization of total tRNA, specific tRNAs, and small molecular weight RNAs. Filled with tables and figures, it presents comparative methods and provides an overview of the progress from a historical perspective. The text features chapters on special and recent methods, isolation of tRNA from specific sources, such as chloroplasts and mitochondria, purification, and identification of modified nucleotides. It also covers suppressor tRNAs, aminoacyl tRNA synthetases, and tRNA genes. This volume is an excellent resource for all biological chemists, microbiologists, and researchers.
Isolation, Fractionation, and Characterization of Transfer RNA. Purification of tRNA Using Antibodies. Purification and Identification of Higher Plant Organellar Transfer RNA. Nucleotide Sequence Analysis and Identification of Modified Nucleotides in tRNA. Mutant Glycine tRNAs and Other Wonders of Translational Suppression. Aminoacyl-tRNA Synthetases. Transfer RNA Genes. Diversity in Soluble RNAs. Small Nuclear RNA.