1st Edition

Vertebrate Skeletal Histology and Paleohistology

    838 Pages 334 Color & 68 B/W Illustrations
    by CRC Press

    838 Pages 334 Color & 68 B/W Illustrations
    by CRC Press

    838 Pages 334 Color & 68 B/W Illustrations
    by CRC Press

    Vertebrate Skeletal Histology and Paleohistology summarizes decades of research into the biology and biological meaning of hard tissues, in both living and extinct vertebrates. In addition to outlining anatomical diversity, it provides fundamental phylogenetic and evolutionary contexts for interpretation. An international team of leading authorities review the impact of ontogeny, mechanics, and environment in relation to bone and dental tissues. Synthesizing current advances in the biological problems of growth, metabolism, evolution, ecology, and behavior, this comprehensive and authoritative volume is built upon a foundation of concepts and technology generated over the past fifty years.




    1. Paleohistology: An Historical – Bibliographical Introduction

    Armand J. de Ricqlès


    2. An Overview of the Embryonic Development of the Bony Skeleton

    Vivian de Buffrénil and Alexandra Quilhac

    3. The Vertebrate Skeleton: A Brief Introduction

    Michel Laurin, Alexandra Quilhac and Vivian de Buffrénil

    Methodological Focus A: The New Scalpel: Basic Aspects of CT-Scan Imaging

    Damien Germain and Sandrine Ladevèze

    4. Microanatomical Features of Bones and Their Basic Measurement

    Vivian de Buffrénil, Eli Amson, Alexandra Quilhac, Dennis Voeten and Michel Laurin

    Methodological Focus B: Basic Aspects of 3D Histomorphometry

    Eli Amson and Damien Germain

    5. Bone Cells and Organic Matrix

    Louise Zylberberg

    6. Current Concepts of the Mineralization of Type I Collagen in Vertebrate Tissues

    William J. Landis, Tengteng Tang and Robin DiFeo Childs

    7. An Overview of Cartilage Histology

    Alexandra Quilhac

    Methodological Focus C: Virtual (Paleo-)Histology Through Synchrotron Imaging

    Sophie Sanchez, Dennis F. A. E. Voeten, Damien Germain and Vincent Fernandez

    8. Bone Tissue Types: A Brief Account of Currently Used Categories

    Vivian de Buffrénil and Alexandra Quilhac

    Methodological Focus D: FIB-SEM Dual-Beam Microscopy for Three-Dimensional Ultrastructural Imaging of Skeletal Tissues

    Natalie Reznikov and Katya Rechav


    9. Basic Processes in Bone Growth

    Vivian de Buffrénil and Alexandra Quilhac

    10. Accretion Rate and Histological Features of Bone

    Vivian de Buffrénil, Alexandra Quilhac and Jorge Cubo

    11. Bone Remodeling

    Vivian de Buffrénil and Alexandra Quilhac

    12. Remarks on Metaplastic Processes in the Skeleton

    Vivian de Buffrénil and Louise Zylberberg


    13. Histology of Dental Hard Tissues

    Alan Boyde and Timothy G. Bromage


    14. Introduction

    Michel Laurin

    15. Finned Vertebrates

    Jorge Mondéjar-Fernández and Philippe Janvier

    16. Early Tetrapodomorphs

    Sophie Sanchez, François Clarac, Michel Laurin and Armand de Ricqlès

    17. Lissamphibia

    Vivian de Buffrénil and Michel Laurin

    18. Early Amniotes and Their Close Relatives

    Aurore Canoville, Michel Laurin and Armand de Ricqlès

    19. Testudines

    Torsten M. Scheyer and Ignacio A. Cerda

    20. Lepidosauria

    Vivian de Buffrénil and Alexandra Houssaye

    21. Sauropterygia: Placodontia

    Torsten M. Scheyer and Nicole Klein

    22. Sauropterygia: Nothosauria and Pachypleurosauria

    Torsten M. Scheyer, Alexandra Houssaye and Nicole Klein

    23. Sauropterygia: Histology of Plesiosauria

    P. Martin Sander and Tanja Wintrich

    24. Ichthyosauria

    P. Martin Sander

    25. Archosauromorpha: From Early Diapsids to Archosaurs

    Armand de Ricqlès, Vivian de Buffrénil and Michel Laurin

    26. Archosauromorpha: The Crocodylomorpha

    Vivian de Buffrénil, Michel Laurin and Stéphane Jouve

    27. Archosauromorpha: Avemetatarsalia – Dinosaurs and Their Relatives

    Kevin Padian and Holly N. Woodward

    28. Nonmammalian Synapsids

    Jennifer Botha and Adam Huttenlocker

    29. Diversity of Bone Microstructure in Mammals

    Vivian de Buffrénil, Christian de Muizon, Maïténa Dumont, Michel Laurin and Olivier Lambert


    30. Phylogenetic Signal in Bone Histology

    Jorge Cubo, Lucas J. Legendre and Michel Laurin

    31. Cyclical Growth and Skeletochronology

    Vivian de Buffrénil, Alexandra Quilhac and Jacques Castanet

    32. Aging and Senescence Processes in the Skeleton

    Catherine Bergot and Vivian de Buffrénil

    33. Basic Principles and Methodologies in Measuring Bone Biomechanics

    Russell P. Main

    34. Interpreting Mechanical Function in Extant and Fossil Long Bones

    Russell P. Main, Erin L.R. Simons and Andrew H. Lee

    35. Bone Microanatomy and Lifestyle in Tetrapods

    Aurore Canoville, Vivian de Buffrénil and Michel Laurin

    36. Bone Histology and the Adaptation to Aquatic Life in Tetrapods

    Alexandra Houssaye and Vivian de Buffrénil

    37. Bone Histology and Thermal Physiology

    Jorge Cubo, Adam Huttenlocker, Lucas J. Legendre, Chloé Olivier and Armand de Ricqlès

    38. Bone Ornamentation: Deciphering the Functional Meaning of an Enigmatic Feature

    François Clarac

    39. The Histology of Skeletal Tissues as a Tool in Paleoanthropological and Archaeological Investigations

    Ariane Burke and Michelle S. M. Drapeau

    40. A Methodological Renaissance to Advance Perennial Issues in Vertebrate Paleohistology

    Alexandra Houssaye, Donald Davesne and Aurore Canoville

    Extended Table of Contents


    Vivian de Buffrénil received a double university degree: history, through a master’s degree, specialized in the history of sciences (Paris 1972), and biology, through a PhD (Paris, 1980) as well as a “thèe d’éat” (Paris 1990). His professional career began in 1982 as a “maîre de conferences” at the Musém National d’Histoire Naturelle, Paris, where he remained until 1986. Since the beginning, Buffréil’s research activity has been related to comparative bone histology and paleohistology in extant and extinct mammals, reptiles and amphibians. About one hundred scientific articles were published on this subject. In parallel, Buffrénil took an active part in international programs led by CITES and FAO (two UN institutions) on the conservation of exploited reptiles, especially African monitor lizards and crocodiles and produced many expert reports.

    Louise Zylberberg received her university degrees at Le Centre national de la recherche scientifique with a double curriculum in biology and biochemistry. She defended her doctoral thesis in histology there in 1968. Her career began as a researcher at the CNRS (1961) and she continued as “directrice de recherche” (1977) until her retirement (2001). She is still active as “emeritus directrice de recherche”. Her research has focused on comparisons of the results obtained with conventional histology and more specialized ultrastructural techniques. Since joining the “Formations squelettiques” team in 1980, she has applied these techniques to the study of mineralized tissues of the skeleton of extant and extinct species including reptiles, amphibians and “fishes”. She is also familiar with immunological techniques and comparative histological and cytological analyses which have revealed the wide variety of skeletal tissues and possible relationships between these various tissues during development and during evolution. She has published about two hundred articles.

    Kevin Padian is Professor in the Department of Integrative Biology and Curator at the Museum of Paleontology at the University of California, Berkeley. He is the author or co-author of dozens of peer reviewed scientific journal articles and editor/co-editor of nearly a dozen books.

    Armand de Ricqlès got his university degrees in Paris with a double concenration in biology and earth sciences. He started his career at the Sciences Faculty of the University of Paris (1961-70) in comparative anatomy and histology, then at the University of Paris VII Denis Diderot (1970-95) where he defended his doctoral thesis in Paleohistology (1973) and got a full professorship in Evolutionary Biology (1983). There he developed a research team, "formations squeletiques," that became famous in developing the comparative histology of bone. He has been a Visiting Professor at the University of Chicago and at the University of California, Berkeley. In 1995 he was elected Professor at the prestigious Collège de France (Paris) as the Chair of "Historical Biology and Evolutionism" until 2010. During his career his interests in research and teaching, as well as popularization of science, have covered the fields of zoology, ecology, vertebrate paleontology, comparative anatomy and histology, phylogenetic systematics and, especially, the paleohistology of tetrapod vertebrates, a research field that he has largely expanded and introduced in several countries. Still currently active in this field, he has published several hundred papers in scientific research and popularization.

    "… the most ambitious and comprehensive survey of bone paleohistology, designed to be relevant to those working on extant species too. There is no other book on the subject anywhere close to it in its broad scope.

    It is wonderful to see a field that flourishes based on new information and careful consideration of the biology and taphonomy of the evidence.

    This book hopefully stimulates by its sheer size and scope ..."

    Marcelo R. Sánchez-Villagra in Swiss Journal of Palaeontology, v. 141, 2022


    "The texts … are very good, of course, but the illustrations are outstanding.  There is so much information presented … that a proper summary of their contents would be a book in itself.  … the greatest value of the book comes from the sheer number of photographs and other images of thin sections of different bone tissues …"

    James Farlow in Priscum e-newsletter of the Paleontological Society, Feb 2023.