Visualization of Nucleic Acids: 1st Edition (Hardback) book cover

Visualization of Nucleic Acids

1st Edition

By Gerard Morel

CRC Press

384 pages

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Hardback: 9780849347818
pub: 1995-03-30

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This book presents a review of the principle approaches for visualizing DNA and RNA. Using scanning tunneling and atomic force microscopes, the three-dimensional image of the surface of nucleic acids can be seen at atomic-scale resolutions. Spreading methods provide useful details on structural features of isolated molecules, but the major constituent of living matter is water, and the cryomicroscope makes it possible to look at DNA in its aqueous environment. Genes can be detected simultaneously in situ in chromosomes using fluorescent probes, and also at the electron microscopic level. In cells, nucleic acids are localized and quantified by dyes; electron microscopy is used with cytochemical, immunocytological, nuclease, and in situ hybridization methods. The main potential applications for pathological studies are shown with particular aspects such as viral nucleic acids and in situ PCR.

Table of Contents

The Classic Methods of Nucleic Acid Visualization for Light Microscopy, J.-M. Exbrayat

Imaging Nucleic Acids with Scanning Probe Microscopes, W.M. Heckl and A. Engel

The Spreading of Nucleic Acids, E. Delain and E. Le Cam

DNA in Its Aqueous Environment, A. Leforestier and J. Dubochet

Ultrastructural Cytochemistry, M. Derenzini

In Situ Detection of Nucleic Acids by the Nuclease-Gold Method, C. Cheniclet, S. Garzon, and M. Bendayan

Ultrastructural Detection of Nucleic Acids by Immunocytology, M. Thiry

Visualization of DNA in Living Cells: Cytometric Approaches, S. Paillasson, J.M. Millot, M. Manfait, and X. Ronot

Dynamical Study of Nucleic Acids by Autoradiography, Thomas, Cavalier, Gouranton

Molecular Biological Techniques Used in the Visualization of Nucleic Acids, C. Perrett

In Situ Hybridization on Chromosomes: Clinical Applications, J. Chiesa and J.P. Bureau

Ultrastructural In Situ Hybridization on Chromosomes, M. Wu

Detection of Nucleic Acid Sequences on Whole Cells with or without Genomic Amplification, G. Lizard, Y. Chardonnet, and D. Schmitt

In Situ Hybridization for Electron Microscopy, G. Morel, D. Le Guellec, H. Mertani, and A. Trembleau

Nonisotopic In Situ Hybridization: A Powerful Tool for Diagnosis of Viral Infection in Routine Pathology, P. Brousset, G. Daste, D. Roda, and J. Selves

Visualization and Identification of Viral Nucleic Acids, F. Puvion-Dutilleul

Ultrastructural Detection of Nucleic Acid in the Glomerular Basement Membrane of Lupus Nephritis, D. Malide and M. Bendayan

Nucleic Acids-Ligand Interactions, E. Le Cam and E. Delain

Subject Categories

BISAC Subject Codes/Headings:
MEDICAL / Biochemistry
MEDICAL / Pathology
SCIENCE / Microscopes & Microscopy