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Visualization of Nucleic Acids



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ISBN 9780849347818
Published March 30, 1995 by CRC Press
384 Pages

 
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Book Description

This book presents a review of the principle approaches for visualizing DNA and RNA. Using scanning tunneling and atomic force microscopes, the three-dimensional image of the surface of nucleic acids can be seen at atomic-scale resolutions. Spreading methods provide useful details on structural features of isolated molecules, but the major constituent of living matter is water, and the cryomicroscope makes it possible to look at DNA in its aqueous environment. Genes can be detected simultaneously in situ in chromosomes using fluorescent probes, and also at the electron microscopic level. In cells, nucleic acids are localized and quantified by dyes; electron microscopy is used with cytochemical, immunocytological, nuclease, and in situ hybridization methods. The main potential applications for pathological studies are shown with particular aspects such as viral nucleic acids and in situ PCR.

Table of Contents

The Classic Methods of Nucleic Acid Visualization for Light Microscopy, J.-M. Exbrayat
Imaging Nucleic Acids with Scanning Probe Microscopes, W.M. Heckl and A. Engel
The Spreading of Nucleic Acids, E. Delain and E. Le Cam
DNA in Its Aqueous Environment, A. Leforestier and J. Dubochet
Ultrastructural Cytochemistry, M. Derenzini
In Situ Detection of Nucleic Acids by the Nuclease-Gold Method, C. Cheniclet, S. Garzon, and M. Bendayan
Ultrastructural Detection of Nucleic Acids by Immunocytology, M. Thiry
Visualization of DNA in Living Cells: Cytometric Approaches, S. Paillasson, J.M. Millot, M. Manfait, and X. Ronot
Dynamical Study of Nucleic Acids by Autoradiography, Thomas, Cavalier, Gouranton
Molecular Biological Techniques Used in the Visualization of Nucleic Acids, C. Perrett
In Situ Hybridization on Chromosomes: Clinical Applications, J. Chiesa and J.P. Bureau
Ultrastructural In Situ Hybridization on Chromosomes, M. Wu
Detection of Nucleic Acid Sequences on Whole Cells with or without Genomic Amplification, G. Lizard, Y. Chardonnet, and D. Schmitt
In Situ Hybridization for Electron Microscopy, G. Morel, D. Le Guellec, H. Mertani, and A. Trembleau
Nonisotopic In Situ Hybridization: A Powerful Tool for Diagnosis of Viral Infection in Routine Pathology, P. Brousset, G. Daste, D. Roda, and J. Selves
Visualization and Identification of Viral Nucleic Acids, F. Puvion-Dutilleul
Ultrastructural Detection of Nucleic Acid in the Glomerular Basement Membrane of Lupus Nephritis, D. Malide and M. Bendayan
Nucleic Acids-Ligand Interactions, E. Le Cam and E. Delain

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