1st Edition
Transcriptomics from Aquatic Organisms to Humans
Novel molecular techniques, such as next-generation sequencing, are used to measure gene expression after exposure to a certain stimulus. Data using these gene expression techniques are highly accurate, sensitive and generate transcriptional profiles from species including humans, fish and crustaceans. This book includes transcriptomic studies of non-infectious and infectious diseases affecting humans and environmental and physiological correlates affecting shrimp and fish aquaculture. The book is intended for undergraduate and graduate students interested in one of the various research areas transformed by transcriptomics, including human disease, fish and crustacean physiological, environmental and farming issues.
Key Features
- Documents the utility of next-generation sequencing and RNA-seq to a wide array of aquatic environmental and physiological issues as well as to human health
- Provides insights into the ways transcriptomics can contribute to the understanding of various research subjects such as aquatic animals, fish ecology and human diseases
- Presents an account of the evolution of the techniques used to determine the transcriptome in crustacean aquaculture
- Describes the mechanisms of genetic interactions between different pathogens and the human host and their effects modifying gene expression levels
1. Shrimp transcriptomics: genome and physiological features
Cesar Marcial Escobedo-Bonilla
2. Transcriptomics as a mechanism to study crustacean host-pathogen interactions
José Reyes Gonzalez-Galaviz, Jesús Guadalupe García-Clark, Cesar Marcial Escobedo-Bonilla et al
3. Fish Transcriptomics: Applied to our Understanding of Aquaculture
Joseph Heras
4. Transcriptomics applied in research of non-communicable disease
Ana Karen González-Palomo, Juan Carlos Fernández-Macias; Velia Verónica Rangel-Ramírez et al
5. The impact of microRNAs on human diseases
Rodolfo Iván Valdez Vega, Jorge Montiel Montoya, José Luis Acosta Rodríguez
6. Transcriptomics to elucidate the mechanisms of pathogen-human interaction
Libia Zulema Rodriguez-Anaya & Ángel Josué Félix-Sastré
Biography
Dr. Libia Zulema Rodríguez-Anaya is a Doctor in Biotechnology Sciences from Instituto Tecnologico de Sonora, and a Lecturer of molecular biology, genetics and environmental microbiology since January 2016. She specializes in comparative analysis of complete virus genomes using next generation sequencing techniques. She has good command of bioinformatic tools for genome curation and molecular epidemiology studies. She is a CONACYT-Research Fellow, developing projects on structural and functional genomics applied to diagnoses and/or treatments for pathogenic free-living (FLA) organisms affecting humans. Dr Rodríguez-Anaya has authored and co-authored four peer-reviewed international papers on molecular characterization, pathogenicity and genomic mechanisms of viruses affecting shrimp and FLA-causing granulomatous amebic encephalitis and keratitis in humans.
Dr. César Marcial Escobedo Bonilla has a PhD in Veterinary Sciences from Ghent University, Belgium, and an MSc in Aquaculture and Environmental Management, CIAD Mazatlán. BSc Biology, UNAM ENEP Iztacala. He is a tenured researcher level C at the Aquaculture Department, IPN-CIIDIR Sinaloa, and a Member of the National Researchers System level I (Mexico). He has authored and co-authored twenty-four indexed papers, two book chapters on aquaculture and shrimp biology, and forty-two works in National and International congresses. He is a Lecturer at graduate level in courses related to aquaculture, and Theses director at bachelor level (2), MSc level (6) and PhD level (1). His research interests include infectious diseases of shrimp, bivalves, frogs and biocontrol of agricultural pests using viruses and entomopathogens. He is a project evaluator at national and international levels, a participant in committees evaluating books, and a reviewer of articles for international Journals listed in the Journal Citation Report and CONACyT. Dr. Bonilla is a member of scientific societies in aquaculture and virology. He won the 2008 prize for the most consulted paper in the Journal of Fish Diseases.
Mark F. Sanders, PhD(UC Davis College of Biological Sciences)
**Description**
This volume describes several applications of transcriptomics, the study of the
RNA content of cells to understand the extent and impact of gene expression.
The book contains six chapters that are evenly split between chapters
describing transcriptomic studies in marine species prominent in aquaculture
and chapters describing transcriptomic studies concerning human health and
disease.
**Purpose**
The authors intend to portray the variety of applications of transcriptomics.
These surveys include examination of the transcriptomes of humans and marine
organisms in health and disease as well as in response to pathogens. This is an
objective with a limited appeal as the two halves of the book will be of
interest to distinct audiences. The scientific quality of the chapters is high
and, in that sense, the goals of the authors are achieved.
**Audience**
In broad terms, biological scientists interested in applications of
transcriptomics are the audience for this book. With its coverage of topics
equally split (three chapters each) between marine organisms important in
aquaculture and human transcriptomics, there is no single audience readily
apparent for both halves of the book. The authors are knowledgeable, the
quality of the writing is high, and the chapters are detailed and well
referenced.
**Features**
This book is fewer than 180 pages and is divided into six chapters. Chapters 1
through 3 deal with transcriptomic studies in aquaculture species (fish and
crustaceans). The topics include differential responses to environmental
changes (chapter 1), host-pathogen interactions (chapter 2), and domestication
and selective breeding (chapter 3). Chapters 4 through 6 describe
transcriptomic approaches to issues of human health and disease. These topics
include transcriptomics in human non-communicable diseases (chapter 4),
transcriptomics of micro-RNAs in human non-communicable diseases (chapter 5),
and human-pathogen interactions (chapter 6). The transcriptomic focus of the
book is split into two distinct taxonomic areas, but the quality of writing is
high.
**Assessment**
This is a book of good quality but limited appeal due primarily to its overall
brevity and its split between aquacultural species and humans.
