The openings offered by functional genomics reconciles organism biology and molecular biology, in order to define an integrative biology that should allow new insights about how a phenotype is built up from a genotype in interaction with its environment. This book covers a wide area of concepts and methods in genomics. This range from international genome sequencing projects to invaluable bioinformatics tools, analytical methods of gene expression, including final metabolic products, and their specific tissue to cellular compartmentalization. This new knowledge enables readers to understand the integration of basic physiological functions, and developmental programs. Also, the establishment of tight relationships between genomics, and genetics and plant breeding reveals synergies for exploiting molecular markers, for analysing genetic variation, or for studying quantitative traits. The integration that is now feasible by these new approaches also effects ecophysiology, and opens up new perspectives for the us
Foreword by Michel Caboche
Foreword by A Charcosset
Preface
Structural Genomics and in silico Analysis
1. Physical Structure of the Plant Nuclear Genome
M. Delseny, M. Echeverria, E. Lasserre
2. The Sequencing of Plant Nuclear Genomes
N. Choisne, S. Samain, N. Demange, G. Orjeda, L. Michelet,
E. Pelletier, M. Salanoubat, J. Weissenbach, F. Quetier
3. Introduction to Bioinformatics
H. Chiapello, F. Rodolphe
4. Databases in Biology
H. Chiapello
5. Gene Prediction
P. Nicolas, H. Chiapello
6. Detecting Protein Function from Genome Sequences
J.F. Gibrat, A. Marin
7. Large-scale Protein Sequence Comparisons
J.L. Risler, S. Pasek, P. Brézellec, A. Louis
Functional Genomics: From Sequence to
Function in Plants
8. Gene Transfer to Plants
Y. Chupeau, M.R. Davey
9. Mutant Libraries: A High-throughput
Arabidopsis Reverse Genetics System
F. Granier, D. Bouchez
10. DNA Microarrays in Plants
P. Doumas, Y. Al-Ghazi, C. Rothan, S. Robin
11. Statistical Design and the Analysis of
Gene Expression Determined by Independent
Component Analysis
A. Riva, A.S. Carpentier, B. Torrésani, A. Hénaut
12. Proteomics
M. Zivy, J. Joyard, M. Rossignol
13. Tracking Gene Expression in Plant Cells:
Microscopy and Associated Bio-imaging Techniques
S. Brown, S. Bolte, B. Satiat-Jeunemaître
14. Tracking Gene Expression in Plant Cells:
New Probes for Functional Genomics
S. Bolte, Y. Boutté, S. Kluge, S. Brown, B. Satiat-Jeunemaître
15. Metabolomics
A. Krapp, J.F. Morot-Gaudry, S. Boutet, G. Bergot,
C. Lelarge, J.L. Prioul, G. Noctor
16. Metabolic Fingerprinting and Profiling by Proton NMR
A. Moing, C. Deborde, D. Rolin
17. Methods for the Qualitative and Quantitative
Analysis of Metabolic Fluxes
M. Dieuaide-Noubhani, A.P. Alonso, C. Roby, P. Raymond
18. The Structure of Metabolic Networks or (Almost) All that is Known
about the Simplest Metabolic Network
Jean-Pierre Mazat
Plant Models in Genomics
19. The Model Species, Arabidopsis thaliana
X. Johnson, D. Bouchez
20. Rice: A Model Plant for Cereal Genomics
M. Delseny
21. The Model Medicago truncatula: from Plant-Microbe
Symbioses to Crop Legume Breeding Boosted by
Cross-Legume Genomics
E.P. Journet, P. Gamas
22. Tomato: A Model Plant for Solanaceae Genomics
R. Stevens, M. Bouzayen, M. Causse, C. Etienne, C. Rothan
23. Genomics as a Road for Grapevine Improvement
A.F. Adam-Blondon, C. Romieu, A. Bouquet
24. Sugarcane: A Tropical Crop with a Highly Complex Genome
J.Y. Hoarau, G. Souza, A. D’ Hont, M. Menossi,
L. Rossini Pinto, A. Pereira de Souza, L. Grivet,
C.F. Martins Menck, E. Cesar Ulian, M. Vincentz
Genomics, Genetic Variability and Plant Breeding
25. Molecular Markers and High-throughput Genotype Analysis
M. Falque, S. Santoni
26. Transposable Elements and the Analysis of Plant Biodiversity
S.M. Tam, C. Mhiri, M.–A. Grandbastien
27. Analysis of Complex Characters into Elementary
Components through the Use of Molecular Quantitative
Genetics in Arabidopsis thaliana
O. Loudet, F. Daniel-Vedele
28. Characterization of Major Genes and QTLs by a
Candidate Gene Approach
R. Stevens, S. Muños, M. Causse, A. Bendahmane, V. Lefebvre
29. Molecular Evolution and Validation of Candidate Gene
D. Manicacci, J.B. Veyrieras, A. Charcosset
30. Genomics and Plant Breeding: Maize and Wheat
B. Hirel, J. Le Gouis, M. Bernard, P. Perez, M. Falque,
F. Quetier, J. Joets, P. Montalent, P. Rogowsky,
A. Murigneux, A. Charcosset
Genomics and Technology
31. From Genomics to Self-assembly
V. Planchot, S.G. Ring, A.J. MacDougall, M. Axelos,
Y. Popineau, P. Colonna
32. Metabolic Fingerprinting and Profiling by
MID-FT-IR (Middle-Fourier Transformed-InfraRed)
G. Mouille and J.N. Johansen
Glossary
C. Meyer, J.F. Morot-Gaudry
List of Contributors
Biography
Morot-Gaudry, J F ; Lea, P; Briat, J.F.