1st Edition

Atlas of Cell Organelles Fluorescence

    208 Pages 48 Color & 188 B/W Illustrations
    by CRC Press

    Containing over 150 original photomicrographs accompanied by protocol information, Atlas of Cell Organelles Fluorescence delineates organelles’ structures, interaction, and organization into complexes. It provides a collection that shows living cells under physiopathological conditions and in the context of treatment with carcinogens, xenobiotics, and chemotherapeutic drugs as well as photosensitizers. A guide for therapeutic, diagnostic, or prognostic interpretation of images, and for further research, the atlas helps you identify critical points of attack and suggests possible strategies for investigation.

    Each section provides a brief introduction and the technical details of the staining methods used. The text identifies the organelles and gives explanations of unusual appearances and the cytochemical reactions when necessary. Additional text is provided where appropriate for extension and implementation of methods being utilized to generate similar results. In all cases the normal and pathological markers and content are presented. The atlas includes a color insert that illustrates characteristic organelle features, microcompartmentalization, and alterations as investigated by one or more probes simultaneously.

    The book, an exhaustive list of cellular events observed using microfluorometry, supplies a compilation of information spread throughout the literature of the last 30 years. Atlas of Cell Organelles Fluorescence brings the information together and puts it in an easily accessible format.

    Contents
    Vital Fluorescence Probes of Cell Organelles
    Mitochondrial Probes
    Lysosomal Probes
    Nuclear Probes

    Metabolic Probes

    Cytotoxic Drugs


    Lysosomotropic Agents
    Mitochondria-Toxic Agents
    Mitochondrial Inhibitors
    Photosensitizers
    Carcinogens and Cancer Chemotherapeutic Drugs: Agents Stimulating the Proliferation of the Endoplasmic Reticulum and Golgi Together with the Loading of Lysosomes

    Genetic Diseases

    Cell Differentiation and Cell Pathology

    Cell-to-Cell Communication

    The Study of Microecosystems

    Biotechnology

    Instrumentation

    Novel Methods and Instrumental Designs


    Two-Photon Excitation Microscopy
    Fourier Interferometry for Excitation-Emission Fluorescence Spectral Imaging
    Excitation–Emission Fluorescence Imaging Combined with Photoacoustic Microscopy. A Combined Fluorescence Imaging and Photoacoustic Microscopy Design for Studies in Cancer Cells
    Combined Confocal Fluorescence and Acoustic Microscopy CCOFAM without Necessity for Scanning
    Is the Study of Nanocompartments in Living Cells Feasible?
    Conclusion
    Index

    Biography

    Kohen\, Elli; Santus\, Rene; Hirschberg\, Joseph G.; Ozkutuk\, Nuri