1st Edition
Chemical Approaches to the Synthesis of Peptides and Proteins
Organic chemists working on the synthesis of natural products have long found a special challenge in the preparation of peptides and proteins. However, more reliable, more efficient synthetic preparation methods have been developed in recent years. This reference evaluates the most important synthesis methods available today, and also considers methods that show promise for future applications.
This text describes the state of the art in efficient synthetic methods for the synthesis of both natural and artificial large peptide and protein molecules. Subjects include an introduction to basic topics, linear solid-phase synthesis of peptides, peptide synthesis in solution, convergent solid-phase synthesis, methods for the synthesis of branched peptides, formation of disulfide bridges, and more. The book emphasizes strategies and tactics that must be considered for the successful synthesis of peptides.
Amino Acids and Peptide and Protein Structure
Amino Acid Activation and Coupling
Protection Schemes for Peptide Synthesis
The Synthesis of Peptides
Solid-Phase Peptide Synthesis
The Solid Support
Protection Schemes
Attachment of the First Amino Acid to the Solid Support
Chain Elongation
Cleavage of the Peptide from the Resin
Examples of Protein Synthesis by Linear SPPS
Peptide Synthesis in Solution
Strategic Considerations
Protection Schemes
Chain Elongation
Final Removal of Protecting Groups
Special Approaches to the Synthesis of Peptides in Solution
Examples of Peptide Synthesis in Solution
Convergent Approaches to the Synthesis of Large Peptides and Proteins
Convergent Solid-Phase Peptide Synthesis
The Coupling of Protected Peptide Segments in Solution
Coupling of Minimally Protected Peptides in Aqueous Solution
Chemical Ligation of Peptide Segments
Formation of Disulfide Bridges
Peptides with Disulfide Bridges
Protection of Cysteine in Peptide Synthesis
Chemical Methods for the Formation of Disulfide Bridges
Regioselective Disulfide Bridge Formation
Peptide Libraries
Multiple Peptide Synthesis in Parallel
Peptide Library Techniques
Examples of Peptide Library Synthesisi
Each chapter contains References
Biography
Paul Lloyd-Williams (Universitat de Barcelona, Barcelona, Spain), Fernando Albericio (Parc Cientific de Barcelona, Spain) and Ernest Giralt (Biomedical Research Institute, Barcelona, Spain)
