Optical Tweezers

Methods and Applications

Edited by Miles J. Padgett, Justin Molloy, David McGloin

© 2010 – Chapman and Hall/CRC

508 pages | 336 B/W Illus.

Purchasing Options:
Hardback: 9781420074123
pub: 2010-06-01
US Dollars$188.95

About the Book

The technical development of optical tweezers, along with their application in the biological and physical sciences, has progressed significantly since the demonstration of an optical trap for micron-sized particles based on a single, tightly focused laser beam was first reported more than twenty years ago. Bringing together many landmark papers on the field, Optical Tweezers: Methods and Applications covers the techniques and uses of optical tweezers.

Each section is introduced by a brief commentary, setting the papers into their historical and contemporary contexts. The first two sections explore the pioneering work of Arthur Ashkin and the use of optical tweezers in biological systems. The book then discusses the extensive use of optical tweezers for the measurement of picoNewton forces and examines various approaches for modeling forces within optical tweezers. The next parts explain how optical tweezers are used in colloid science, how to convert optical tweezers into optical spanners, and how spatial light modulators create holographic tweezers. The book concludes with a section on emerging applications of optical tweezers in microfluidic systems.

With contributions from some of the best in the field, this compendium presents important historical and current developments of optical tweezers in a range of scientific areas, from the manipulation of bacteria to the treatment of DNA.


Optical Tweezers: Methods and Applications is a collection of landmark papers in the field of optical trapping and manipulation, edited by three experts of the field. … Optical Tweezers gives a broad overview of the progress over the last four decades through a careful selection of the key articles that every specialist should read. Each section is introduced by a brief but compelling commentary, setting the papers in their historical context and delivering a wealth of background information and references, which can serve as a guide for the interested reader to explore the field further.

—Giovanni Volpe, Contemporary Physics, 52, 2011

Table of Contents

Optical Tweezers: The Early Years

A preliminary communication on the pressure of heat and light radiation, E.F. Nichols and G.F. Hull

Acceleration and trapping of particles by radiation pressure, A. Ashkin

Optical levitation by radiation pressure, A. Ashkin and J.M. Dziedzic

Optical levitation of liquid drops by radiation pressure, A. Ashkin and J.M. Dziedzic

Observation of resonances in radiation pressure on dielectric spheres, A. Ashkin and J.M. Dziedzic

Observation of a single-beam gradient force optical trap for dielectric particles, A. Ashkin, J.M. Dziedzic, J.E. Bjorkholm, and S. Chu

Optical trapping and manipulation of viruses and bacteria, A. Ashkin and J.M. Dziedzic

Optical trapping and manipulation of single cells using infrared-laser beams, A. Ashkin, J.M. Dziedzic, and T. Yamane

Applications in Biology

Elasticity of the red cell membrane and its relation to hemolytic disorders: an optical tweezers study, J. Sleep, D. Wilson, R. Simmons, and W. Gratzer

Lateral movements of membrane-glycoproteins restricted by dynamic cytoplasmic barriers, M. Edidin, S.C. Kuo, and M.P. Sheetz

Compliance of bacterial flagella measured with optical tweezers, S.M. Block, D.F. Blair, and H.C. Berg

Direct observation of steps in rotation of the bacterial flagellar motor, Y. Sowa, A.D. Rowe, M.C. Leake, T. Yakushi, M. Homma, A. Ishijima, and R.M. Berry

Direct observation of kinesin stepping by optical trapping interferometry, K. Svoboda, C.F. Schmidt, B.J. Schnapp, and S.M. Block

Movement and force produced by a single myosin head, J.E. Molloy, J.E. Burns, J. Kendrick-Jones, R.T. Tregear, and D.C.S. White

The gated gait of the processive molecular motor, myosin v, C. Veigel, F. Wang, M.L. Bartoo, J.R. Sellers, and J.E. Molloy

Promoter binding, initiation, and elongation by bacteriophage T7 RNA polymerase, G.M. Skinner, C.G. Baumann, D.M. Quinn, J.E. Molloy, and J.G. Hoggett

Ionic effects on the elasticity of single DNA molecules, C.G. Baumann, S.B. Smith, V.A. Bloomfield, and C. Bustamante

Elasticity and unfolding of single molecules of the giant muscle protein titin, L. Tskhovrebova, J. Trinick, J.A. Sleep, and R.M. Simmons

Measuring Forces and Motion

Optical trapping, K.C. Neuman and S.M. Block

Quantitative measurements of force and displacement using an optical trap, R.M. Simmons, J.T. Finer, S. Chu, and J.A. Spudich

The stiffness of rabbit skeletal actomyosin cross-bridges determined with an optical tweezers transducer, C. Veigel, M.L. Bartoo, D.C.S. White, J.C. Sparrow, and J.E. Molloy

Sequence-resolved detection of pausing by single RNA polymerase molecules, K.M. Herbert, A. La Porta, B.J. Wong, R.A. Mooney, K.C. Neuman, R. Landick, and S.M. Block

Single-molecule mechanics of heavy meromyosin and S1 interacting with rabbit or drosophila actins using optical tweezers, J.E. Molloy, J.E. Burns, J.C. Sparrow, R.T. Tregear, J. Kendrick-Jones, and D.C. White

Mechanics of the kinesin step, N.J. Carter and R.A. Cross

Two-dimensional tracking of ncd motility by back focal plane interferometry, M.W. Allersma, F. Gittes, M.J. deCastro, R.J. Stewart, and C.F. Schmidt

Single kinesin molecules studied with a molecular force clamp, K. Visscher, M.J. Schnitzer, and S.M. Block

Force generation in single conventional actomyosin complexes under high dynamic load, Y. Takagi, E.E. Homsher, Y.E. Goldman, and H. Shuman

Mapping the actin filament with myosin, W. Steffen, D. Smith, R. Simmons, and J. Sleep

Modeling Forces and Torques

Forces of a single beam gradient laser trap on a dielectric sphere in the ray optics regime, A. Ashkin

Parametric study of the forces on microspheres held by optical tweezers, W.H. Wright, G.J. Sonek and M.W. Berns

Optical trapping of dielectric particles in arbitrary fields, A. Rohrbach and E.H.K. Stelzer

Single-beam trapping of microbeads in polarized light: numerical simulations, A.R. Zakharian, P. Polynkin, M. Mansuripur and J.V. Moloney

On the electromagnetic force on a dielectric medium, S.M. Barnett and R. Loudon

Optical tweezers computational toolbox, T.A. Nieminen, V.L.Y. Loke, A.B. Stilgoe, G. Knoner, A.M. Branczyk, N.R. Heckenberg, and H. Rubinsztein-Dunlop

Optical trapping of spheroidal particles in Gaussian beams, S.H. Simpson and S. Hanna

Observation of bistability and hysteresis in optical binding of two dielectric spheres, N.K. Metzger, K. Dholakia and E.M. Wright

Power spectrum analysis for optical tweezers, K. Berg-Sorensen and H. Flyvbjerg

Parametric resonance of optically trapped aerosols, R. Di Leonardo, G. Ruocco, J. Leach, M.J. Padgett, A.J. Wright, J.M. Girkin, D.R. Burnham, and D. McGloin

Studies in Colloid Science

Optical trapping of metallic Rayleigh particles, K. Svoboda and S.M. Block

Measurement of long-range repulsive forces between charged particles at an oil-water interface, R. Aveyard, B.P. Binks, J.H. Clint, P.D.I. Fletcher, T.S. Horozov, B. Neumann, V.N. Paunov, J. Annesley, S.W. Botchway, D. Nees, A.W. Parker, A.D. Ward, and A.N. Burgess

Tailored surfaces using optically manipulated colloidal particles, C. Mio and D.W.M. Marr

Patterning surfaces with colloidal particles using optical tweezers, J.P. Hoogenboom, D.L.J. Vossen, C. Faivre-Moskalenko, M. Dogterom, and A. van Blaaderen

Kinetically locked-in colloidal transport in an array of optical tweezers, P.T. Korda, M.B. Taylor, and D.G. Grier

Measurement of the hydrodynamic corrections to the Brownian motion of two colloidal spheres, J.C. Crocker

Direct measurement of hydrodynamic cross correlations between two particles in an external potential, J.C. Meiners and S.R. Quake

Micromechanics of dipolar chains using optical tweezers, E.M. Furst and A.P. Gast

Optical binding, M.M. Burns, J.M. Fournier, and J.A. Golovchenko

One-dimensional optically bound arrays of microscopic particles, S.A. Tatarkova, A.E. Carruthers, and K. Dholakia

Optical Spanners

Optical trapping and rotational manipulation of microscopic particles and biological cells, S. Sato, M. Ishigure, and H. Inaba

Controlled rotation of optically trapped microscopic particles, L. Paterson, M.P. MacDonald, J. Arlt, W. Sibbett, P.E. Bryant, and K. Dholakia

Rotational control within optical tweezers by use of a rotating aperture, A.T. O’Neil and M.J. Padgett

Direct observation of transfer of angular momentum to absorptive particles from a laser beam with a phase singularity, H. He, M.E.J. Friese, N.R. Heckenberg, and H. Rubinsztein-Dunlop

Mechanical equivalence of spin and orbital angular momentum of light: an optical spanner, N.B. Simpson, K. Dholakia, L. Allen, and M.J. Padgett

Optical alignment and spinning of laser-trapped microscopic particles, M.E.J. Friese, T.A. Nieminen, N.R. Heckenberg, and H. Rubinsztein-Dunlop

Intrinsic and extrinsic nature of the orbital angular momentum of a light beam, A.T. O’Neil, I. MacVicar, L. Allen, and M.J. Padgett

Modulated optical vortices, J.E. Curtis and D.G. Grier

Optically controlled three-dimensional rotation of microscopic objects, V. Bingelyte, J. Leach, J. Courtial, and M.J. Padgett

Optically induced rotation of anisotropic micro-objects fabricated by surface micromachining, E. Higurashi, H. Ukita, H. Tanaka, and O. Ohguchi

Optical microrheology using rotating laser-trapped particles, A.I. Bishop, T.A. Nieminen, N.R. Heckenberg, and H. Rubinsztein-Dunlop

Multitrap and Holographic Optical Tweezers

Micromanipulation by multiple optical traps created by a single fast scanning trap integrated with the bilateral confocal microscope, K. Visscher, G.J. Brakenhoff and J.J. Krol

Construction of multiple-beam optical traps with nanometer-resolution position sensing, K. Visscher, S.P. Gross, and S.M. Block

Optical tweezers and confocal microscopy for simultaneous three-dimensional manipulation and imaging in concentrated colloidal dispersions, D.L.J. Vossen, A. van der Horst, M. Dogterom, and A. van Blaaderen

Optical manipulation of microscopic objects by means of vertical-cavity surface-emitting laser array sources, Y. Ogura, K. Kagawa, and J. Tanida

Optical tweezer arrays and optical substrates created with diffractive optics, E.R. Dufresne and D.G. Grier

Multifunctional optical tweezers using computer-generated holograms, J. Liesener, M. Reicherter, T. Haist, and H.J. Tiziani

Dynamic holographic optical tweezers, J.E. Curtis, B.A. Koss, and D.G. Grier

An optical trapped microhand for manipulating micron-sized objects, G. Whyte, G. Gibson, J. Leach, M.J. Padgett, D. Robert, and M. Miles Assembly of three-dimensional structures using programmable holographic optical tweezers, G. Sinclair, P. Jordan, J. Courtial, M.J. Padgett, J. Cooper, and Z.J. Laczik

Four-dimensional optical manipulation of colloidal particles, P.J. Rodrigo, V.R. Daria, and J. Gluckstad

Optimized holographic optical traps, M. Polin, K. Ladavac, S.H. Lee, Y. Roichman, and D.G. Grier

Applications in Microfluidics

Demonstration of a fiber optic light-force trap, A. Constable, J. Kim, J. Mervis, F. Zarinetchi, and M. Prentiss

The optical stretcher: a novel laser tool to micromanipulate cells, J. Guck, R. Ananthakrishnan, H. Mahmood, T.J. Moon, C.C. Cunningham, and J. Kas

An optically driven pump for microfluidics, J. Leach, H. Mushfique, R. di Leonardo, M.J. Padgett, and J. Cooper

Microfluidic control using colloidal devices, A. Terray, J. Oakey, and D.W.M. Marr

Microfluidic sorting in an optical lattice, M.P. MacDonald, G.C. Spalding, and K. Dholakia

Optical thermal ratchet, L.P. Faucheux, L.S. Bourdieu, P.D. Kaplan, and A.J. Libchaber

Optical chromatography, T. Imasaka, Y. Kawabata, T. Kaneta, and Y. Ishidzu

Microfluidic and optical systems for the on-demand generation and manipulation of single femtoliter-volume aqueous droplets, R.M. Lorenz, J.S. Edgar, G.D.M. Jeffries, and D.T. Chiu

Optical sculpture: controlled deformation of emulsion droplets with ultralow interfacial tensions using optical tweezers, A.D. Ward, M.G. Berry, C.D. Mellor, and C.D. Bain

Control and characterisation of a single aerosol droplet in a single-beam gradient-force optical trap, R.J. Hopkins, L. Mitchem, A.D. Ward, and J.P. Reid

Holographic optical trapping of aerosol droplets, D.R. Burnham and D. McGloin

About the Series

Series in Optics and Optoelectronics

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Subject Categories

BISAC Subject Codes/Headings:
SCIENCE / Life Sciences / Biology / Molecular Biology
SCIENCE / Physics