A Laboratory Course in Biomaterials  book cover
1st Edition

A Laboratory Course in Biomaterials

ISBN 9781420075823
Published June 18, 2009 by CRC Press
216 Pages 123 B/W Illustrations

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Book Description

The field of biomedical engineering has vastly expanded in the past two decades, as reflected in the increased number of bioengineering and biomaterials programs at universities. The growth of this area has outpaced the development of laboratory courses that allow students hands-on experience, since the barriers involved in creating multidisciplinary biomaterials laboratory courses are high. A Laboratory Course in Biomaterials provides a teaching tool comprehensive in scope perspective.

Multidisciplinary approach

Suitable for junior or senior level laboratory courses in biomaterials and bioengineering, this volume trains students in laboratory skills, data analysis, problem solving, and scientific writing. The text takes a multidisciplinary approach, integrating a variety of principles that include materials science, chemistry, biochemistry, molecular and cell biology, and engineering.

Step-by-step instructions

The author presents flexible modules that allow the coursework to be adapted to the needs of different departments. Each module is organized around a central theme, such as drug delivery and natural biomaterials, to enhance student comprehension. This book provides step-by-step descriptions of lab procedures, reagents, equipment, and data processing guidelines. It also includes a series of thought-provoking questions and answers following each experiment, drawn from the author’s own experience in teaching a biomaterials laboratory course at the University of Illinois.

Timely in its coverage, many of the experiments presented in the book are adapted from research papers reflecting the progress in various disciplines of bioengineering and biomaterials science.

Table of Contents

Basic Laboratory Skills

Commonly Used Lab Equipment and Supplies

Waste Disposal

Liquid Transfer


Making Solutions

Error Analysis

Module I. Drug Delivery: Controlled Release of Encapsulated Protein from PLGA Microspheres

Session 1. Encapsulation of BSA in PLGA Microspheres

Session 2. Evaluation of the Encapsulation Efficiency and the Drug Load

Session 3. Controlled Release of BSA from PLGA Microspheres

Session 4. Evaluation of Controlled Release Profile


Appendix. Recipes and Sources for Equipment, Reagents, and Supplies

Module II. Natural Biomaterials: Collagen and Chitosan

Session 1. Extracting Acid-Soluble Type I Collagen from Bovine Calf Skin

Session 2. Purification of Extracted Collagen

Session 3. Analysis of Collagen Extraction and Purification by Electrophoresis

Session 4. Fabricating Collagen/Chitosan Sponges


Appendix. Recipes and Sources for Equipment, Reagents and Supplies

Basic Laboratory Skills II. Cell Culture and Microscopy

Cell Culture


Appendix. Sources and Recipes for Equipment, Reagents, and Supplies

Module III. Biocompatibility Testing: Cytotoxicity and Adhesion

Session 1. Cytotoxicity Evaluation Using Direct Contact Tests

Session 2. Cytotoxicity Evaluation Using Liquid Extracts of Materials

Session 3. Biofunctionality Evaluation through Cell Adhesion/Proliferation Tests


Appendix. Recipes and Sources for Equipment, Reagents and Supplies

Module IV. Tissue Engineering: Organotypic Cultures of Skin Equivalents

Session 1. The Dermis Equivalent

Session 2. The Epidermis Equivalent

Session 3. Histological Studies of Natural Skin and Organotypic Skin Equivalent


Appendix A. Sterilizing Collagen and Preparing Collagen Solution

Appendix B. Custom-Made Teflon Support for Air Exposure of the Epidermis Equivalent

Appendix C. Recipes and Sources for Equipment, Reagents and Supplies

Module V. Bioceramics: Porous Hydroxyapatite Composite

Session 1. Synthesis of HA and FA

Session 2. X-Ray Powder Diffraction of HA and FA

Session 3. Fabrication of Porous HA/FA-PLGA Composite Using a Salt-Leaching Method

Session 4. Characterization of the Porous HA/FA-PLGA Composites—Acid Degradation and Compression Testing


Appendix. Recipes and Sources for Equipment, Reagents and Supplies

Appendix: Answers


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Wujing Xian is a professor at the University of Illinois at Urbana-Champaign.