Among the many recent advances in assisted reproduction therapies (ART), improved technologies for identifying viable oocytes, sperm, and embryos are of primary importance. Paradoxically, the latest advances presented at conferences and symposia are often slow to become part of the daily routine in IVF laboratories. Detailing established and developing techniques, A Practical Guide to Selecting Gametes and Embryos provides a user-friendly text of ready-to-use ARTs that can be utilized effectively in the lab.
In this volume, renowned embryologist and educator Markus Montag and his expert panel highlight sophisticated and proven selection strategies and emphasize the importance of proper lab practice in handling gametes and embryos.
- Steps undertaken for the analysis of a semen sample
- Quality control and prevention of exposure to toxins in oocyte collection and embryo culture
- Morphological selection of gametes and embryos
- Both commonly used and innovative techniques for gamete and embryo selection, such as oxygen respiration and time-lapse imaging
- Invasive techniques, including polar body, embryo, and blastocyst biopsies as well as aneuploidy testing by FISH and array-CGH
Accompanied by numerous figures and descriptions, this guide to selecting gametes and embryos brings the insight of international authors with knowledge and expertise, highlighting practical tips and key points. The book offers a starting point for applying successful selection strategies for reducing the rate of high-risk multiple gestations while maintaining or increasing viable pregnancy rates.
Table of Contents
Handling Gametes and Embryos: Sperm Collection and Preparation Techniques; Verena Nordhoff, Con Mallidis, and Sabine Kliesch
Handling Gametes and Embryos: Oocyte Collection and Embryo Culture; Lars Johansson
Handling Gametes and Embryos: Quality Control for Culture Conditions; Jason E. Swain
Morphological Selection of Gametes and Embryos: Sperm; Pierre Vanderzwalmen, Magnus Bach, Olivier Gaspard, Bernard Lejeune, Anton Neyer, Françoise Puissant, Maximilian Schuff, Astrid Stecher, Sabine Vanderzwalmen, Barbara Wirleitner, and Nicolas H Zech
Morphological Selection of Gametes and Embryos: Oocyte; Basak Balaban and Thomas Ebner
Morphological Selection of Gametes and Embryos: 2PN / Zygote; Martin Greuner and Markus Montag
Morphological Selection of Gametes and Embryos: Embryo; Gayle Jones and M. Cristina Magli
Morphological Selection of Gametes and Embryos: Blastocyst; Thomas Ebner
Noninvasive Techniques: Gamete Selection—Sperm; Victoria Sánchez, Joachim Wistuba, and Con Mallidis
Noninvasive Techniques: Gamete Selection—Oocyte; Laura Rienzi, Benedetta Iussig, and Filippo Maria Ubaldi Noninvasive Techniques: Embryo Selection by Oxygen Respiration; Alberto Tejera, Belén Aparicio, Carmela Albert, Arancha Delgado, and Marcos Meseguer
Noninvasive Techniques: Embryo Selection by Time-Lapse Imaging; Alison Campbell
Noninvasive Techniques: Embryo Selection by Transcriptomics, Proteomics, and Metabolomics; Asli Uyar and Emri Seli
Invasive Techniques: Polar Body Biopsy; Markus Montag, Jana Liebenthron, and Maria Köster
Invasive Techniques: Embryo Biopsy at the Cleavage Stage; Anick DeVos
Invasive Techniques: Blastocyst Biopsy; Steve McArthur
Invasive Techniques: Aneuploidy Testing by FISH; Semra Kahraman and Çağrı Beyazyürek Invasive Techniques: Aneuploidy Testing by Array-CGH; Alan R. Thornhill, Christian Ottolini, Gary Harton, and Darren Griffin
Summary: Comprehensive Summary Of Main Points by Topic
" … a user-friendly text of ready-to-use ARTs that can be utilized effectively in the lab."
"As an experienced embryologist, I enjoyed the book—the information is applicable to everyday laboratory issues … The text is comprehensive and offers numerous visual aids such as tables and photographic images to guide readers … I have a revitalized outlook on how I view embryos for transfer and I am eager to use these pointers."
—Bridget Cerimovic for Doody's Review Service