This comprehensive monograph consists of two parts: Volume I, entitled Enzyme Catalysis, Kinetics, and Substrate Binding; and Volume II, entitled Mechanism of Enzyme Action. Volume I focuses on several aspects of enzyme catalytic behavior, their steady-state and transient-state kinetics, and the thermodynamic properties of substrate binding. Packed with figures, tables, schemes, and photographs, this volume contains over 1,000 references, including references regarding enzymology's fascinating history. This comprehensive book is of particular interest to enzymology students, teachers, and researchers.
Volume II presents selected "cutting edge" examples of techniques and approaches being pursued in biochemistry. This up-to-date resource includes 11 chapters, which illustrate important theoretical and practical aspects of enzyme mechanisms. It also features selected examples in which today's most important techniques, ideas, and theories are used to elaborate on the intricate nature of enzyme action mechanisms. This particular volume provides important information for both the novice and the seasoned investigator.
Some Theoretical Aspects of Enzyme Mechanisms: Mechanical Force, Hydration, and Conformational Fluctuations in Enzymic Catalysis (R. Lumry). Complexation and Catalysis in Biology (R.J.P. Williams). Mechanistic Role of Biotin in Enzymatic Carboxylation Reactions (A.S. Mildvan, D.C. Fry, and E.H. Serpersu). Thiamin-Dependent Reactions (Mechanisms) (C.J. Gubler). Allostery and Non-Michaelis-Menten Behavior: Glycogen Phosphorylase and Glycogen Synthetase (N.B. Madsen). Proteases-Structures and Mechanisms: Calcium-Activated Neutral Protease (K. Imahori, K. Suzuki, and S. Kawashima). Dehydrogenases-Structure and Catalytic Mechanisms: Glutamate Dehydrogenase (Bovine Liver) (R.F. Colman). Dihydrofolate Reductase (J.F. Morrison). 2-Oxoacid Dehydrogenases (M. Hamada and H. Takenaka). Flavoprotein Catalysis: Old Yellow Enzyme (L.M. Schopfer and V. Massey). D-Amino Acid Oxidase (K. Yagi). Cytochrome c, Quinone, and Cytochrome P-450 Reductases (M.S. Johnson and S.A. Kuby). Activated Oxygen Reactions: Dioxygenases and Monooxygenases (S. Yamamoto and Y. Ishimura). ATP-ases, Nucleotide Binding Carriers, and Nucleotidyl Transferases: Ca2+, Mg2+-ATPase (Microsomal) (H. Takenaka, M. Sumida, and M. Hamada). Mechanistic and Energetic Aspects of Carrier Catalysis-Exemplified with Mitochondrial Translocators (M. Klingenberg). Terminal Deoxynucleotidyl Transferase (M.J. Modak and V.N. Pandey). Phosphotransferases-Structure and Mechanisms: Adenylate Kinase (M. Hamada, H. Takenaka, M. Sumida, and S.A. Kuby). Phosphofructokinase and Fructose 6-Phosphate, 2-Kinase: Fructose 2,6-Bisphosphatase (K. Uyeda). Emden-Meyerhof Reactions-Mechanism of Action: Triose- and Hexose-Phosphate Isomerases (K.Ü. Yüksel and R.W. Gracy). Mechanistic Studies of Muscle Aldolase (D.J. Hupe). Metalloenzymes: Mechanism and Structure of Superoxide Dismutases (J.A. Tainer, V.A. Roberts, C.L. Fisher, R.A. Hallewell, and E.D. Getzoff). Hydrolases-Mechanisms: Phospholipases (G. Arthur and P.C. Choy). Esterases (Including Choline Esterases) (H. Okuda). Lipases (H. Okuda). Index.