Immunologists as well investigators in other disciplines may often use protocols involving the isolation, cultures and characterization of different types of leukocytes. Advanced Methods in Cellular Immunology is a collection of techniques in an easy-to-use format.
Each chapter provides readers with related program information, a step-by-step description of the methodology, alternative techniques, pertinent references, and information about commercial sources for materials and regents. In addition to leukocytes, the authors guide readers through the processes of cell culture as well as inflammation and autoimmunity in a variety of animal models.
Covering topics such as PCR and Apoptosis, this book will serve a guide to commonly used procedures in cellular immunology while utilizing both human and murine models.
Table of Contents
Sources. Isolation and Characterization of Neutrophils. Isolation and Characterization of Eosinophils. Enrichment of Human Basophils. Macrophages. B Lymphocyte Cloning. Cell Cultivation In-Vitro. Analysis of Cytokine or Cytokine Receptor mRNA. Intracellular Cytokine Staining. Cell Cytotoxicity. Mixed Lymphocyte Reaction. Animal Models of Autoimmune Diseases. Buffers and Media. Commercial Sources. Index.