1st Edition

Advanced Microscopy Photo-Thermal and Induced-Raman Microscopy

By Takayoshi Kobayashi Copyright 2025
    432 Pages 20 Color & 139 B/W Illustrations
    by CRC Press

    This book covers the principle, structure, enhancement of sensitivity and resolution power of photothermal and Raman microscopies. It includes real-world applications to biological and medical targets.

    Advanced Microscopy: Photo-Thermal and Induced-Raman Microscopy introduces clear descriptions of various Raman processes such as spontaneous, stimulates, coherent anti-Stokes Raman (CARS), Raman loss and Stokes Raman (gain). It covers pump-probe microscopies using actinic (pump) laser and sensing (probe) laser resulting in improvement due to intrinsic nonlinearity, which provides an advantage in the imaging of nonfluorescent targets. The author also provides solutions to noise and sensitivity problems which are two most important concerns in the microscopy applications. Finally, the book also draws direct comparisons of the advantages and drawbacks of a Raman microscopes in comparison with photothermal microscopes.

    The book will be useful to researchers and non-specialists in biomedical fields using optics and electronics relevant to (optical) microscopes. It will also be a helpful resource to graduate students in the fields of biology and medical research who are using photothermal microscopes in their research.

    Chapter 1- Preface & Introduction

    Chapter 2.1.1- Optimal detection angle in sub-diffraction resolution photothermal microscopy: application for high sensitivity imaging of biological tissues

    Chapter 2.1.2- Resolution enhanced pump-probe microscope with a spatial filter

    Chapter 2.1.3- Reduction of distortion in photothermal microscopy and its application to the high- resolution three-dimensional imaging of nonfluorescent tissues

    Chapter 2.2.1- Numerical study of the subtraction threshold for fluorescence difference microscopy

    Chapter 2.2.2- Sub-diffraction-limit imaging using mode multiplexing

    Chapter 2.2.3- Polarization modulation for fluorescence emission difference microscopy

    Chapter 2.2.4- Numerical study of super-resolved optical microscopy with partly staggered beams

    Chapter 2.2.5- Resolution enhancement of pump-probe microscopy with an inverse-annular spatial filter

    Chapter 3.1- Sub-diffraction resolution pump-probe microscopy with shot-noise limited sensitivity using laser diodes

    Chapter 3.2- Sensitivity enhancement of photothermal microscopy with radially segmented balanced detection

    Chapter 3.3- Fast 3D visualization of endogenous brain signals with high-sensitivity laser scanning photothermal microscopy

    Chapter 4.1.1- Numerical calibration of the spatial overlap for subtraction microscopy

    Chapter 4.1.2- Polarization modulation for fluorescence emission difference microscopy

    Chapter 4.1.3- Subtraction threshold for an isotropic fluorescence emission difference microscope

    Chapter 4.2- Noise cancellation with phase-detection technique for pump-probe measurement and application to stimulated Raman imaging

    Chapter 4.3- Simultaneous dual-wavelength imaging of nonfluorescent tissues with 3D subdiffraction photothermal microscopy

    Chapter 5.1- Gray-level co-occurrence matrix analysis of several cell types in mouse brain using resolution-enhanced photothermal microscopy

    Chapter 5.2- Label-free imaging of melanoma with nonlinear photothermal microscopy

    Chapter 5.3- Noninvasive, label-free, three-dimensional imaging of melanoma with confocal photothermal microscopy: Differentiate malignant melanoma from benign tumor tissue

    Chapter 5.4- Label-Free Imaging of Melanoma with Confocal Photothermal Microscopy: Differentiation between Malignant and Benign Tissue

    Chapter 6.1- Development of a multiplex stimulated Raman microscope for spectral imaging through multi-channel lock-in detection

    Chapter 6.2- Multiplex stimulated Raman imaging with white probe-light from a photonic-crystal fibre and with multi-wavelength balanced detection

    Chapter 6.3- Theoretical description for nonlinear dynamic light scattering based on stimulated Raman effect (NLDLS-SRS) O Number 662


    Takayoshi Kobayashi is a Professor Emeritus of The University of Tokyo, Chair Professor of National Chiao-Tung University, and guest professors at the University of Electro-Communications and Tokyo University of Science. His research interest include quantum electronics, laser physics, femtosecond spectroscopy, ultrafast nonlinear optics, quantum optics, chemical physics, quantum information science and technology.