Advances in Chromatographic Techniques for Therapeutic Drug Monitoring  book cover
1st Edition

Advances in Chromatographic Techniques for Therapeutic Drug Monitoring

Edited By

Amitava Dasgupta

ISBN 9781138111714
Published June 16, 2017 by CRC Press
475 Pages 92 B/W Illustrations

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Book Description

For drugs with a narrow therapeutic index, therapeutic drug monitoring methods are essential for patient management. Although immunoassays are commercially available for many drugs and most laboratories use these assays for routine therapeutic monitoring, they have many limitations which hinder their efficacy. Providing practical guidelines for implementing preferred gas and liquid chromatographic methods, Advances in Chromatographic Techniques for Therapeutic Drug Monitoring is a comprehensive reference describing the theory and application of therapeutic drug monitoring in clinical laboratories.

Edited by a distinguished authority in the field and containing contributions from a variety of experts, the book discusses preanalytical variables, the pitfalls of immunoassays, tandem mass spectrometry, issues related to pain management and herbal supplements, and therapeutic drug monitoring for a range of medications, including:

  • Anticonvulsants
  • Digitalis
  • Cardioactive drugs
  • Antidepressants
  • Immunosuppressants
  • Anti-cancer drugs
  • Vancomycin and aminoglycosides
  • Antibiotics
  • Antiretroviral drugs
  • Nonnarcotic analgesics
  • Anti-inflammatory drugs


Examing older and newer drugs, the book contains detailed discussions on the rationale for therapeutic drug monitoring of each class of drugs, along with their basic pharmacology and toxicology. An extensive list of references is provided at the end of each chapter so that those interested in implementing a new drug assay can find the most appropriate method for the intended drug.

Table of Contents

Introduction to Therapeutic Drug Monitoring and Chromatography, A. Dasgupta

Preanalytical Variables and Therapeutic Drug Monitoring, V. Bush

Immunoassays for Therapeutic Drug Monitoring: Pitfalls and Limitations, P. Datta and A. Dasgupta

Introduction to Tandem Mass Spectrometry, G. L. Hortin

Analytical Support of Classical Anticonvulsant Drug Monitoring beyond Immunoassay: Application of Chromatographic Methods, J. Juenke and G. McMillin

Clinical Practice of Therapeutic Drug Monitoring of New Anticonvulsants, M. D. Krasowski

Chromatographic Techniques in the Therapeutic Drug Monitoring of New Anticonvulsants, C. L. H. Snozek and L. J. Langman

Problems with Digoxin Immunoassays, A. Dasgupta

Liquid Chromatography Combined with Immunoassay as a Reference Method for Analysis of Digitalis, E. P. Womack, R. Valdes, and S. A. Jortani

Chromatographic Methods for Analysis of Cardioactive Drugs, R. W. McLawhon

Immunoassays for Tricyclic Antidepressants: Unsuitable for Therapeutic Drug Monitoring?, M. D. Krasowski, M. G. Siam, and S. Ekins

Chromatographic Techniques for the Analysis of Antidepressants, U. Garg

Pharmacokinetics and Therapeutic Drug Monitoring of Immunosuppressants, K. A. Kelly and A. W. Butch

Analysis of Immunosuppressants by Liquid Chromatography or Liquid Chromatography with Mass Spectrometry, K. N. Eaton

Therapeutic Drug Monitoring in Cancer Patients: Application of Chromatographic Techniques, A. Dasgupta

Therapeutic Drug Monitoring of Vancomycin and Aminoglycosides with Guidelines, R. Dean and A. Dasgupta

Chromatographic Methods for Analysis of Antibiotics, R. W. McLawhon

Need for Therapeutic Drug Monitoring of Antiretroviral Medications in HIV Infection, N. Y. Rakhmanina and J. N. van den Anker

Chromatographic Techniques for Therapeutic Drug Monitoring of Antiretroviral Drugs, A. Dasgupta

Chromatography in Therapeutic Drug Monitoring of Nonnarcotic Analgesics and Anti-inflammatory Drugs, U. Garg

Therapeutic Drug Monitoring in Pain Management: Application of Chromatography and Mass Spectrometry, C. L. H. Snozek and L. J. Langman

Investigation of Drug–herb Interactions: Application of various Chromatographic Techniques for Analysis of Active Components of Herbal Supplements, A. Dasgupta

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Dr. Amitava Dasgupta is a Professor of Pathology and Laboratory Medicine at the University of Texas Health Sciences Center at Houston. He is also the Director of Clinical Chemistry, Toxicology, and Point of Care Services at the Memorial-Hermann Hospital Laboratories at the University of Texas Medical School at Houston. He received his PhD in chemistry from Stanford University and completed his fellowship in clinical chemistry from the University of Washington at Seattle. He is certified in both clinical chemistry and toxicology by the American Board of Clinical Chemistry. Dr. Dasgupta’s major research interest is in the field of therapeutic drug monitoring and he has published 175 papers and numerous reviews and has edited four books. He is on the editorial board of American Journal of Clinical Pathology, Archives of Pathology and Laboratory Medicine, Therapeutic Drug Monitoring, Clinica Chimica Acta, and Journal of Clinical Laboratory Analysis.


…This excellent book reviews not only those drugs for which chromatographic techniques have long been used, but also drugs for which therapeutic monitoring is newer (e.g., antiretroviral agents, immunosuppressive drugs, antineoplastic compounds, antidepressant drugs, analgesics, cardioactive drugs, and antibiotics). … Edited by a distinguished authority on therapeutic drug monitoring, this book should serve as both an invaluable reference for analytical toxicologists and a practical guide for laboratory physicians and scientists who are involved in the monitoring of therapeutic agents.
—From the Foreword, David N. Bailey, Vice Chancellor for Health Affairs, Dean of the School of Medicine, and Professor of Pathology & Laboratory Medicine, University of California, Irvine, USA

… a valuable reference for experienced clinical laboratory professionals, as well as an opportunity to inform a new generation of scientists and clinicians of the future potential and growing importance of clinical TDM.
—Michael Hodsdon in Future Science, May 2010, vol. 2, no. 5