1st Edition

Advances in Chromatography Volume 19

Edited By J. Calvin Giddings Copyright 1981

    This book provides up-to-date information on a wide range of developments in liquid, gel permeation and hydrophobic interaction chromatographic methods and applications. It presents cutting-edge reviews in the fields of bio-, analytical, organic, polymer, and pharmaceutical chemistry.

    Contents of Other Volumes
    Chapter 1
    Roles of High-Performance Liquid Chromatography in Nuclear Medicine
    Steven How-Yan Wong
    I. Introduction
    II. Characteristics of Radiopharmaceuticals
    III. Instrumentation
    IV. Preparation and Purification
    V. Analysis of ⁹⁹ᵐTc-Labeled Pharmaceuticals
    VI. Conclusion
    Chapter 2
    Calibration of Separation Systems in Gel Permeation Chromatography for Polymer Characterization
    Josef Janca
    I Introduction
    II. Calibration Using Standards with Narrow Distributions
    III. Calibration Using Standards with Broad Distribution
    IV. Universal Calibration
    V. Special Procedures of Calibration
    VI. Influence of Operational Variables on Results of Calibration
    VII. Conclusions
    Appendix: Applications of Universal Calibration Principles in Synthetic Polymer Characterization
    Chapter 3
    Isomer-Specific Assay of 2, 4-D Herbicide Products
    By HPLC: Regulatory Methodology
    Timothy S. Stevens
    I. Introduction
    II. Ion-Exchange Chromatography Versus Revers-Phase Chromatography
    III. Method Criteria
    IV. Impurities in 2, 4-D
    V. Separation of Impurities
    VI. Alternative Columns
    VII. Column Stability
    VIII. Precision and Linearity
    IX. Mobile Phase Modifier
    X. Conclusions
    Chapter 4
    Hydrophobic Interaction Chromatography
    Stellan Hjerten
    I. Introduction
    II. Definition of Hydrophobic Interaction and the Structure of Water
    III. Changes in the Water Structure upon Solubilization of a Nonpolar Compound in Water
    IV. Chemical Nature of the Hydrophobic Interaction
    V. Charged Amphiphilic Adsorbents
    VI. Neutral Amphiphilic Adsorbents
    VII. Multipoint Interaction
    VIII. Establishment of Appropriate Experimental Conditions for a Run
    IX. Applications
    Chapter 5
    Liquid Chromatography with Programmed Composition of the Mobile Phase
    Pavel Jandera and Jaroslav Churacek
    I. Introduction
    II. Retention Characteristics in Isocratic-Elution Chromatography and in Chromatography with Programmed Composition of the Mobile Phase
    III. Practical Aspects of Chromatography with Programmed Composition of the Mobile Phase
    IV. Conclusions
    V. Summary
    Appendix A: List of Symbols Used
    Chapter 6
    Chromatographic Separation of Aldosterone and its Metabolites
    David J. Morris and Ritsuko Tsai
    I. Introduction
    II. Metabolism and Mechanism of Action of Aldosterone
    III. Separation and Column Chromatography of Reduced and Polar Aldosterone Metabolites
    IV. High-Pressure Liquid Chromatography of Reduced and Polar Aldosterone Metabolites
    V. Improved Separations of Aldosterone Metabolites Using High-Pressure Liquid Chromatography
    VI. High-Pressure Liquid Chromatography of Aldosterone of Aldosterone Metabolites Synthesized in Vitro
    VII. Aldosterone Metabolites in Male Rat Urine
    VIII. Conclusions
    Author Index
    Subject Index


    J. Calvin Giddings (University of Utah, Salt Lake City, USA)