1st Edition

Antimicrobial Susceptibility Testing Protocols

    428 Pages 68 B/W Illustrations
    by CRC Press

    The clinical microbiology laboratory is often a sentinel for the detection of drug resistant strains of microorganisms. Standardized protocols require continual scrutiny to detect emerging phenotypic resistance patterns.  The timely notification of clinicians with susceptibility results can initiate the alteration of antimicrobial chemotherapy and improve patient care. It is vital that microbiology laboratories stay current with standard and emerging methods and have a solid understanding of their function in the war on infectious diseases.

    Antimicrobial Susceptibility Testing Protocols clearly defines the role of the clinical microbiology laboratory in integrated patient care and provides a comprehensive, up-to-date procedural manual that can be used by a wide variety of laboratorians. The authors provide a comprehensive, up-to-date procedural manual including protocols for bioassay methods and molecular methods for bacterial strain typing. Divided into three sections, the text begins by introducing basic susceptibility disciplines including disk diffusion, macro and microbroth dilution, agar dilution, and the gradient method. It covers step-by-step protocols with an emphasis on optimizing the detection of resistant microorganisms. The second section describes specialized susceptibility protocols such as surveillance procedures for detection of antibiotic-resistant bacteria, serum bactericidal assays, time-kill curves, population analysis, and synergy testing. The final section is designed to be used as a reference resource. Chapters cover antibiotic development; design and use of an antibiogram; and the interactions of the clinical microbiology laboratory with the hospital pharmacy, and infectious disease and control.

    Unique in its scope, Antimicrobial Susceptibility Testing Protocols gives laboratory personnel an integrated resource for updated lab-based techniques and charts within the contextual role of clinical microbiology in modern medicine.

    An Overview of the Clinical and Laboratory Standards Institute and its Impact on Antimicrobial Susceptibility Testing, A. L. Barry
    Antimicrobial Classifications: Drugs for Bugs, C.B. Calderón, and B. Perdue Sabundayo
    Disk Diffusion Tests and Gradient Methodologies, A. Wanger
    Macro and Microdilution Methods of Antimicrobial Susceptibility Testing, S. Qaiyumi
    Automated Systems: An Overview, S. Stuckey
    Agar Dilution Susceptibility Testing, A. Hanlon, M. Taylor, and J. Dick
    Antibiograms/Preventive Surveillance: A Continuum of Data Collection, Analysis, and Presentation, J. Thomas and N.T. Rector
    Anaerobe Susceptibility Testing, D. E. Roe-Carpenter
    Antifungal Susceptibility Testing of Yeasts, A. Espinel-Ingroff and E. Cantón
    Antifungal Susceptibility Testing of Filamentous Fungi, A. Espinel-Ingroff and E. Cantón
    Susceptibility Testing of Mycobacteria, B. A. Brown-Elliott, S. Cohen, and R.J. Wallace, Jr.
    Methods for Determining Bactericidal Activity and Antimicrobial Interactions: Synergy Testing/Time-Kill Curves/Population Analysis, P. Verma
    Serum Bactericidal Testing, H. Nadler and M. Dowzicky
    Bioassay Methods for Antimicrobial and Antifungal Agents, D.H. Pitkin and E. Martin-Mazuelos
    Molecular Methods for Bacterial Strain Typing, S. Michaud and D. Berg
    Pharmacy and Microbiology: Interactive Synergy, B. Perdue Sabundayo and C.B. Calderón
    Interactions Between Clinicians and the Microbiology Laboratory, J.H. Powers
    Clinical Microbiology in the Development of New Antimicrobial Agents, C.D.Webb and B.G. Painter


    Lynn Steele-Moore, Richard Schwalbe, Avery C. Goodwin

    "… exceptionally well written both in clarity and through illustration. … there is a well-developed index that readers will find quite useful for subject searches. … this book is a comprehensive manual of antimicrobial susceptibility testing protocols and applications. I believe it will be of great value for clinical microbiologists and pathologists, physicians, veterinarians, and pharmacists, medical and veterinary technologists, molecular biologists, infectious disease epidemiologists, as well as infection control practitioners and hospital administrators."

    – Christian T.K.-H. Stadtlander, University of St. Thomas, Minnesota in Microbe, January 2009