Antimicrobial Susceptibility Testing Protocols  book cover
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1st Edition

Antimicrobial Susceptibility Testing Protocols




ISBN 9780824741006
Published May 22, 2007 by CRC Press
432 Pages - 68 B/W Illustrations

 
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Book Description

The clinical microbiology laboratory is often a sentinel for the detection of drug resistant strains of microorganisms. Standardized protocols require continual scrutiny to detect emerging phenotypic resistance patterns.  The timely notification of clinicians with susceptibility results can initiate the alteration of antimicrobial chemotherapy and improve patient care. It is vital that microbiology laboratories stay current with standard and emerging methods and have a solid understanding of their function in the war on infectious diseases.

Antimicrobial Susceptibility Testing Protocols clearly defines the role of the clinical microbiology laboratory in integrated patient care and provides a comprehensive, up-to-date procedural manual that can be used by a wide variety of laboratorians. The authors provide a comprehensive, up-to-date procedural manual including protocols for bioassay methods and molecular methods for bacterial strain typing. Divided into three sections, the text begins by introducing basic susceptibility disciplines including disk diffusion, macro and microbroth dilution, agar dilution, and the gradient method. It covers step-by-step protocols with an emphasis on optimizing the detection of resistant microorganisms. The second section describes specialized susceptibility protocols such as surveillance procedures for detection of antibiotic-resistant bacteria, serum bactericidal assays, time-kill curves, population analysis, and synergy testing. The final section is designed to be used as a reference resource. Chapters cover antibiotic development; design and use of an antibiogram; and the interactions of the clinical microbiology laboratory with the hospital pharmacy, and infectious disease and control.

Unique in its scope, Antimicrobial Susceptibility Testing Protocols gives laboratory personnel an integrated resource for updated lab-based techniques and charts within the contextual role of clinical microbiology in modern medicine.

Table of Contents

An Overview of the Clinical and Laboratory Standards Institute and its Impact on Antimicrobial Susceptibility Testing, A. L. Barry
Antimicrobial Classifications: Drugs for Bugs, C.B. Calderón, and B. Perdue Sabundayo
Disk Diffusion Tests and Gradient Methodologies, A. Wanger
Macro and Microdilution Methods of Antimicrobial Susceptibility Testing, S. Qaiyumi
Automated Systems: An Overview, S. Stuckey
Agar Dilution Susceptibility Testing, A. Hanlon, M. Taylor, and J. Dick
Antibiograms/Preventive Surveillance: A Continuum of Data Collection, Analysis, and Presentation, J. Thomas and N.T. Rector
Anaerobe Susceptibility Testing, D. E. Roe-Carpenter
Antifungal Susceptibility Testing of Yeasts, A. Espinel-Ingroff and E. Cantón
Antifungal Susceptibility Testing of Filamentous Fungi, A. Espinel-Ingroff and E. Cantón
Susceptibility Testing of Mycobacteria, B. A. Brown-Elliott, S. Cohen, and R.J. Wallace, Jr.
Methods for Determining Bactericidal Activity and Antimicrobial Interactions: Synergy Testing/Time-Kill Curves/Population Analysis, P. Verma
Serum Bactericidal Testing, H. Nadler and M. Dowzicky
Bioassay Methods for Antimicrobial and Antifungal Agents, D.H. Pitkin and E. Martin-Mazuelos
Molecular Methods for Bacterial Strain Typing, S. Michaud and D. Berg
Pharmacy and Microbiology: Interactive Synergy, B. Perdue Sabundayo and C.B. Calderón
Interactions Between Clinicians and the Microbiology Laboratory, J.H. Powers
Clinical Microbiology in the Development of New Antimicrobial Agents, C.D.Webb and B.G. Painter

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Reviews

"… exceptionally well written both in clarity and through illustration. … there is a well-developed index that readers will find quite useful for subject searches. … this book is a comprehensive manual of antimicrobial susceptibility testing protocols and applications. I believe it will be of great value for clinical microbiologists and pathologists, physicians, veterinarians, and pharmacists, medical and veterinary technologists, molecular biologists, infectious disease epidemiologists, as well as infection control practitioners and hospital administrators."

– Christian T.K.-H. Stadtlander, University of St. Thomas, Minnesota in Microbe, January 2009