2nd Edition

Applying Genomic and Proteomic Microarray Technology in Drug Discovery

By Robert S. Matson Copyright 2013
    322 Pages 200 B/W Illustrations
    by CRC Press

    Microarrays play an increasingly significant role in drug discovery. The commercial landscape has changed dramatically over the past few years and researchers have made great advancements with regard to construction and use. Now in its second edition, Applying Genomic and Proteomic Microarray Technology in Drug Discovery highlights, describes, and evaluates current scientific research using microarray technology in genomic and proteomic applications.

    Updated and revised to reflect recent progress in the field, the second edition discusses:

    • Expanded omics-driven applications, including the areas of metabolomics and chemical biology
    • The commercialization of the microarray platform, with a historical perspective aimed at recognizing key technological developments
    • Solid-supports (substrates) and surface chemistries currently used in the creation of nucleic acid and protein microarrays
    • Different approaches to producing microarrays that achieve spot equality with the same number of molecules properly oriented
    • The development of the gene expression microarray and representative applications
    • The development of protein microarray technology, including its history and key applications

    Unique to this edition is a new chapter on multiplex assays that examines the development and applications of arrays across diverse platforms. It discusses applications for qPCR, multiplex lateral flow, and multiplex bead assays. It also presents platform-to-platform comparisons.

    Microarrays remain an invaluable tool for omics-based research not only in drug discovery, but in the life sciences, in clinical research, and for diagnostic applications worldwide. This volume presents the current state of the art on the utility of this technology to solve a host of important biological problems.

    Omics and Microarrays Revisited
    The Microarray Format
    The Omic Era
    The Role for Gene Expression Microarrays in Drug Discovery
    Proteomics Today—The Great Challenge
    The Potential Role for Protein Microarrays in Drug Discovery
    Future Medicine—Pharmacoproteomics or Pharmacogenomics?
    Commercial Microarrays
    In Situ DNA Arrays
    Ex Situ or Spotted DNA Arrays
    Comparison of Commercial DNA Microarrays
    Commercial Protein Arrays
    Three-Dimensional (3D) and Four-Dimensional (4D) Chips
    Flow-Thru Biochips
    Electronic Biochips
    Supports and Surface Chemistries
    Physical Features
    Surface Chemistries
    Variation in the Performance of Glass Slide-Based Antibody Microarrays
    Comparison of Different Surface Chemistries for the Immobilization of Auto-Antigens
    Click Chemistry as an Immobilization Strategy
    Oxygen Plasma-Mediated Modification of DVD-R Disks for Tethering of Oligonucleotides
    Construction of Lipid Bilayer Microarrays
    Arraying Processes
    Creating Spotted Microarrays
    Microarray Printing Mechanisms
    Microarray Pins
    Other Approaches
    Setting Up the Print Run
    Protocols for Printing Nucleic Acids
    Protocols for Printing Proteins
    Newer Methods for Printing
    Gene Expression: Microarray-Based Applications
    Applications Demonstrating DNA Microarray Utility
    Biomedical Research Applications
    Array-Based Comparative Genomic Hybridization
    Protein Microarray Applications
    Applications Demonstrating Protein Microarray Utility
    Measuring Microarray Performance
    Other Microarray Formats Useful for Proteomic Applications
    Dual Labeling of Targets for Increased Sensitivity and Specificity
    The Depletion of Highly Abundant Proteins from Serum Deemed Unnecessary
    Competitive ELISA by Protein Microarray
    The Issue of Cross-Reactivity in a Protein Microarray Sandwich ELISA
    Multiplex Assays
    Multiplex Polymerase Chain Reaction (PCR)
    Multiplex Lateral Flow
    Multiplex Bead-Based Assays
    Multiplex Microarrays
    Adoption of Multiplex Assays


    Robert S. Matson is president and co-founder of QuantiScientifics, LLC. The company is involved in the commercialization of multiplexed assays for life science, clinical research, and in vitro diagnostics. Previously, Matson was involved in the research and development of microarray technologies, detection chemistries, as well as point-of-care devices for more than 17 years while at Beckman Coulter, Inc. He participated in the National Institute of Standards and Technology’s (NIST) Advanced Technology Program sponsored Genosensor Consortium and collaborated with Sir Edwin Southern on the development of an in situ oligonucleotide array synthesis platform for the corporation. Other work included development of the A2 MicroArray System, a microplate-based array platform for multiplexed micro-ELISA, which QuantiScientifics recently licensed for commercialization.

    "This publication offers a detailed perspective and insight into the present and future uses of this technology"
    Anticancer Research

    "A basic reference on the benefits of microarray technology in drug discovery, this publication offers a detailed perspective and insight into the present and future uses of this technology."
    Anticancer Research