Applying Genomic and Proteomic Microarray Technology in Drug Discovery: 2nd Edition (Hardback) book cover

Applying Genomic and Proteomic Microarray Technology in Drug Discovery

2nd Edition

By Robert S. Matson

CRC Press

322 pages | 200 B/W Illus.

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pub: 2013-03-13
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Description

Microarrays play an increasingly significant role in drug discovery. The commercial landscape has changed dramatically over the past few years and researchers have made great advancements with regard to construction and use. Now in its second edition, Applying Genomic and Proteomic Microarray Technology in Drug Discovery highlights, describes, and evaluates current scientific research using microarray technology in genomic and proteomic applications.

Updated and revised to reflect recent progress in the field, the second edition discusses:

  • Expanded omics-driven applications, including the areas of metabolomics and chemical biology
  • The commercialization of the microarray platform, with a historical perspective aimed at recognizing key technological developments
  • Solid-supports (substrates) and surface chemistries currently used in the creation of nucleic acid and protein microarrays
  • Different approaches to producing microarrays that achieve spot equality with the same number of molecules properly oriented
  • The development of the gene expression microarray and representative applications
  • The development of protein microarray technology, including its history and key applications

Unique to this edition is a new chapter on multiplex assays that examines the development and applications of arrays across diverse platforms. It discusses applications for qPCR, multiplex lateral flow, and multiplex bead assays. It also presents platform-to-platform comparisons.

Microarrays remain an invaluable tool for omics-based research not only in drug discovery, but in the life sciences, in clinical research, and for diagnostic applications worldwide. This volume presents the current state of the art on the utility of this technology to solve a host of important biological problems.

Table of Contents

Omics and Microarrays Revisited

The Microarray Format

The Omic Era

The Role for Gene Expression Microarrays in Drug Discovery

Proteomics Today—The Great Challenge

The Potential Role for Protein Microarrays in Drug Discovery

Future Medicine—Pharmacoproteomics or Pharmacogenomics?

Commercial Microarrays

In Situ DNA Arrays

Ex Situ or Spotted DNA Arrays

Comparison of Commercial DNA Microarrays

Commercial Protein Arrays

Three-Dimensional (3D) and Four-Dimensional (4D) Chips

Flow-Thru Biochips

Electronic Biochips

Supports and Surface Chemistries

Substrates

Physical Features

Surface Chemistries

Variation in the Performance of Glass Slide-Based Antibody Microarrays

Comparison of Different Surface Chemistries for the Immobilization of Auto-Antigens

Click Chemistry as an Immobilization Strategy

Oxygen Plasma-Mediated Modification of DVD-R Disks for Tethering of Oligonucleotides

Construction of Lipid Bilayer Microarrays

Arraying Processes

Creating Spotted Microarrays

Microarray Printing Mechanisms

Microarray Pins

Other Approaches

Setting Up the Print Run

Protocols for Printing Nucleic Acids

Protocols for Printing Proteins

Newer Methods for Printing

Gene Expression: Microarray-Based Applications

Applications Demonstrating DNA Microarray Utility

Biomedical Research Applications

Micro-RNA

Array-Based Comparative Genomic Hybridization

Protein Microarray Applications

Applications Demonstrating Protein Microarray Utility

Measuring Microarray Performance

Other Microarray Formats Useful for Proteomic Applications

Dual Labeling of Targets for Increased Sensitivity and Specificity

The Depletion of Highly Abundant Proteins from Serum Deemed Unnecessary

Competitive ELISA by Protein Microarray

The Issue of Cross-Reactivity in a Protein Microarray Sandwich ELISA

Multiplex Assays

Multiplex Polymerase Chain Reaction (PCR)

Multiplex Lateral Flow

Multiplex Bead-Based Assays

Multiplex Microarrays

Adoption of Multiplex Assays

Index

About the Author

Robert S. Matson is president and co-founder of QuantiScientifics, LLC. The company is involved in the commercialization of multiplexed assays for life science, clinical research, and in vitro diagnostics. Previously, Matson was involved in the research and development of microarray technologies, detection chemistries, as well as point-of-care devices for more than 17 years while at Beckman Coulter, Inc. He participated in the National Institute of Standards and Technology’s (NIST) Advanced Technology Program sponsored Genosensor Consortium and collaborated with Sir Edwin Southern on the development of an in situ oligonucleotide array synthesis platform for the corporation. Other work included development of the A2 MicroArray System, a microplate-based array platform for multiplexed micro-ELISA, which QuantiScientifics recently licensed for commercialization.

Subject Categories

BISAC Subject Codes/Headings:
MED071000
MEDICAL / Pharmacology
MED072000
MEDICAL / Pharmacy