1st Edition
Assessing Bacterial Growth Potential in Seawater Reverse Osmosis Pretreatment Method Development and Applications
Seawater desalination is increasingly being used as a means to augment freshwater supplies in regions with high water stress, and reverse osmosis is increasingly the technology of choice because of the low energy consumption. However, seawater reverse osmosis (SWRO) systems suffer from various types of fouling, which can increase energy consumption and the use of chemicals during SWRO operation. In practice, pre-treatment systems are put in place to reduce the particulate and biological fouling potential of SWRO feed water. However, simple, reliable and accurate methods to assess the extent to which biological fouling potential is reduced during pre-treatment are not available for seawater.
This research developed a new method to measure bacterial growth potential (BGP) using the native bacterial consortium in seawater. New reagents to extract and detect ATP in microbial cells were specifically developed for seawater. The new lysis and detection reagents overcame the salt interference in seawater and allow low detection of total ATP, free ATP and microbial ATP in seawater. Incorporating a filtration step further increased the sensitivity of the method six fold, enabling ATP detection of ultra-low levels of microbial ATP in seawater.
The newly developed ATP-based BGP method was applied to monitor and assess the pre-treatment of five full-scale seawater desalination plants around the world. A good correlation was observed between BGP measured in SWRO feed water and the pressure drop increase in the SWRO systems, suggesting the applicability of using the ATP-based BGP method as a biofouling indicator in SWRO. Furthermore, a safe level of BGP (<70 µg/L) is proposed for SWRO feed water in order to ensure a chemical cleaning frequency of once/year or lower. However, to validate this conclusion, more SWRO plants with different pre-treatment systems need to be monitored.
In the future, on-line monitoring of BGP in SWRO feed water may further reduce the consumption of chemicals and energy and improve the overall sustainability of seawater desalination by reverse osmosis.
1. Introduction and thesis outline
1.1 Freshwater availability
1.2 Seawater desalination
1.3 Fouling of reverse osmosis
1.4 Monitoring of biofouling
1.5 Controlling of biofouling
1.6 Bacterial growth methods
1.7 Bacterial enumeration methods
1.8 Adenosine Triphosphate (ATP)
1.9 Motivation of the study
1.10 Research objectives
1.11 Thesis framework
2. Direct measurement of ATP in seawater
2.1 Introduction
2.2 Materials and methods
2.3 Results and discussion
2.4 Conclusions
2.5 Acknowledgments
3. Eliminating seawater matrix effects in ATP measurement using a filtration process
3.1 Introduction
3.2 Materials and methods
3.3 Results and discussion
3.4 Conclusions
3.5 Acknowledgments
3.6 Supplementary data
4. Biofouling potential using ATP-based bacterial growth potential method
4.1 Introduction
4.2 Material and methods
4.3 Results and discussion
4.4 Conclusions
4.5 Acknowledgement
4.6 Supplementary material
S4.1 Microbial enumeration method
S4.2 Microbial inactivation processes
S4.3 Inoculum concentration
S4.4 Incubation temperature of Indigenous microorganisms
5 Correlating bacterial growth potential measurement to real time fouling development in full-scale SWRO
5.1 Introduction
5.2 Material and methods
5.3 Result
5.4 Discussion
5.5 Conclusions
5.6 Supplementary materials:
6. Application of ATP and BGP methods to monitor media filtration and dissolved air flotation pre-treatment systems
6.1 Introduction
6.2 Materials and methods
6.3 Results and discussion
6.4 Conclusions
6.5 Annexes
7. Conclusions and future perspective
7.1 Conclusions
7.2 Future perspective
Biography
Abushaban completed his MSc in Municipal water and infrastructure at UNSECO-IHE, Netherlands. He is currently finalizing his PhD at Delft University of Technology, in which his research focused on development of methods to control biofouling and scaling of reverse osmosis (RO) and ultrafiltration membrane systems. His work included bench-scale, pilot-scale and even full-scale plants. Abushaban has extensive experience in assessing and troubleshooting the pre-treatment and membrane filtration performance of full-scale seawater/industrial treatment plants. Abushaban was awarded the innovation prize of the international desalination association (IDA) in 2017. Furthermore, Abushaban has worked as water and wastewater consultant engineer, in which he managed designing and rehabilitation of several water supply and sanitation projects in Palestine.
