1st Edition
Basics of CRISPR/Cas Mediated Plant Genome Editing
CRISPR/Cas has emerged, as a powerful genetic engineering tool. It is simple yet highly affordable and robust. This book provides a basic introduction to the mechanism and architecture of the CRISPR/Cas. It enlightens the readers about the new advancements such as gene insertion, transcriptional activation, suppression, gene tagging, multiplexing, base editing, prime editing, and tissue culture free editing which makes it easier for crop improvement. With such technologies, application of genome editing has opened new paths for crop improvements and sustainable agriculture. The current book will be useful for beginners to understand these tools and how to use them in a better and more efficient way.
Preface
Introduction to Genome Editing Tool in Plants
Navjot Kaur, Zienab Ahmed, Anshu Alok and Jitesh Kumar
The Mechanism of CRISPR and Its Classification
Alka Jangra, Garima Sharma, Siddhant Chaturvedi and Vinod Chhokar
Classification of the CRISPR/Cas System
Mitrabinda Panda, Seema Pradhan and Late. Ajay Parida
Emergence of CRISPR Technology Bringing Revolution in Plant Biotechnology
Nandita Thakur, Phanikanth Jogam and Baohong Zhang
CRISPR/Cas Delivery Methods in Plants
Neetu Singh Kushwah, Shristi Verma, Meenal Rathore and NP Singh
Bioinformatics Approaches to Guide RNA Designing and Off-target Prediction
Vinita Sharma, Himanshu Sharma, Chibuikem Unamba, Joy Roy and Abhishek Bhandawat
Importance of Tissue Culture in Genome Editing
Bushra Hafeez Kiani
Multiplex Genome Editing Approaches for the Plant Genetic Manipulations
Sukhmandeep Kaur, Sanskriti Vats, Bharti Aggarwal, Jashandeep Kaur and Anupriya Bhardwaj
Transcriptional Activation and Suppression using CRISPR/Cas9: A Plant’s Outlook
Ayyagari Ramlal, Hardik Singhal, Kamal Kumar, Lalit Kumar, Vimal Pandey and Sahil Mehta
CRISPR/Cas-Based Genome Editing for Soybean Improvement
Ashita Bisht, Dinesh Kumar Saini, Pankaj Kumar and B S Gill
Application of CRISPR Technology in Plant Salt Tolerance and Membrane Proteins
Debajyoti Dutta and Prabir Kumar Das
Index
Biography
Dr. Anshu Alok has expertise in plant genetic engineering and genome editing. He has used genome editing in tobacco, potato, wheat, and banana. He has authored over 50 peer-reviewed research and review articles, and book chapters. Dr. Alok has served as editor in 3 Biotech, IJMS and still serves on the Editorial Boards of BMC Plant Method and Frontier in Genome editing.
Dr. Jitesh Kumar has expertise in plant biology, particularly genome editing. He has authored over 25 peer-reviewed papers and book chapters. Dr. Kumar actively contributes to the academic community by serving on the Editorial Boards of BMC Plant Biology and Biotechnology.
Dr. Mahipal S. Shekhawat is an expert in the area of plant cell and tissue culture. He is working on the micropropagation of medicinal & horticultural crops, plant structural & behavioral responses, and biosynthesis of nanoparticles and their applications in crop improvement. He has authored over 150 peer-reviewed research papers.
